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Arbuscular mycorrhizal fungi GZ176 for disease prevention, growth promotion and adverse resistance of herbaceous plants and microbial inoculums and application of arbuscular mycorrhizal fungi GZ176

The technology of an arbuscular mycorrhizal fungus, GZ176, is applied in the direction of plant growth regulators, plant growth regulators, botany equipment and methods, etc. It can solve different and different physiological and biochemical characteristics and biological functions, etc., to achieve growth promotion, Promote the sustainable development of agriculture and improve the effect of stress resistance

Active Publication Date: 2015-09-23
南京本源生态农业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this patent utilizes the joint action of four different arbuscular mycorrhizal fungi, and there may be a synergistic effect between the bacteria. Those skilled in the art cannot predict whether using one of the arbuscular mycorrhizal fungi can still play a role in promoting growth and preventing Disease efficacy
And strains from different sources may have different physiological and biochemical characteristics and biological functions, and different plants may produce different effects even if they use the same bacterial agent
Which arbuscular mycorrhizal fungus can produce ideal biocontrol effects on soil-borne and above-ground diseases of herbaceous plants, and improve the stress resistance of herbal plants, there is no relevant report so far

Method used

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  • Arbuscular mycorrhizal fungi GZ176 for disease prevention, growth promotion and adverse resistance of herbaceous plants and microbial inoculums and application of arbuscular mycorrhizal fungi GZ176
  • Arbuscular mycorrhizal fungi GZ176 for disease prevention, growth promotion and adverse resistance of herbaceous plants and microbial inoculums and application of arbuscular mycorrhizal fungi GZ176
  • Arbuscular mycorrhizal fungi GZ176 for disease prevention, growth promotion and adverse resistance of herbaceous plants and microbial inoculums and application of arbuscular mycorrhizal fungi GZ176

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] An arbuscular mycorrhizal fungus GZ176 powder that improves the biological control effect on herbaceous crop diseases, promotes growth, and resists stress. It was isolated from tobacco rhizosphere soil in Guizhou Province.

[0037] Isolation and identification method of AM mycorrhizal fungus GZ176: separate AM fungal spores by wet sieve pouring-sucrose centrifugation, weigh 100g soil sample in a centrifuge tube, add water to the 2 / 3 of the centrifuge tube, centrifuge at 3000rpm for 3min, then remove the top Add 45%-50% sucrose solution to the supernatant, stir well and then centrifuge at 1500rpm for 1.5min quickly, pass through a 400-mesh sieve, and gently wash the spores and spores on the sieve with water, collect them in a petri dish with normal saline, and The number of spores was counted under a microscope and the morphology of the spores was observed.

[0038] Morphological characteristics of GZ176: the spore wall is mostly 3-4 layers, and the outer layer is color...

Embodiment 2

[0040] The preparation method of AM fungus GZ176 powder is as follows:

[0041] 1) Evenly mix the previously propagated soil containing the arbuscular mycorrhizal fungus GZ176 with the culture medium at a volume ratio of 1:100, the matrix formula: peat soil mixed with coarse sand that has been steamed for at least 2 hours;

[0042] 2) Put the mixed spore-containing substrate into the sterilized ground pots (15 cm in diameter), and plant sorghum, with 50-60 seeds in each pot, to ensure that the spores can fully contact the sorghum. After 4 months, the soil in the pot and the sorghum root system were taken out together, dried, pulverized to 800 mesh, and kept dry to prepare the soil containing mycorrhizal fungus spores for subsequent use;

[0043] 3) Preparation: mix the soil of mycorrhizal fungus spores with kaolin according to step 2), dry and pulverize, pass through a 300-mesh sieve, dry again to a water content of 3%, and finally make a concentration of about 50 spores / g p...

Embodiment 3

[0044] Embodiment 3 greenhouse disease prevention test

[0045] Method: The test tomato seeds were treated with 10% H 2 o 2 The surface was disinfected for 10 minutes, washed with sterile water and dried for later use.

[0046] Before sowing, fill 200g of sterilized soil and 10g of GZ176 powder (prepared in Example 2) after mixing in each pot to ensure normal inoculation. 10 seeds were sown in each nutrient pot, and after 4 weeks of normal watering management, the mycorrhizal seedlings were transplanted into 330ml plastic cups, and the plants with the same growth were selected for the experiment when transplanting. 24 seedlings were treated in each group, placed randomly, and repeated three times.

[0047] 3 days after transplanting, inoculate R. solanacearum ZJ3721 (isolated from tomato plants with R. solanacearum in Zhejiang and preserved). Plate activated Ralstonia solanacearum ZJ3721, shake overnight in 500ml YGPA culture solution (200rpm / min, 20-24h, 30°C). Subsequen...

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Abstract

The invention discloses arbuscular mycorrhizal fungi GZ176 for disease prevention, growth promotion and adverse resistance of herbaceous plants and microbial inoculums and application of the arbuscular mycorrhizal fungi GZ176. The arbuscular mycorrhizal fungi GZ176 is rhizophagus intraradices and can be made into powder with concentration being 40-60 spores per gram, herbaceous plant disease prevention rate is up to 71.3% after the arbuscular mycorrhizal fungi GZ176 powder is subjected to seed dressing under normal management conditions of greenhouses, and herbaceous plant growth promotion rate is higher than 16.19%; in the field, disease prevention and control rate reaches 75.27%, and yield increasing rate exceeds 36.17%; adverse resistance of the herbaceous plants can be improved remarkably.

Description

technical field [0001] The invention belongs to the field of plant protection, and relates to an arbuscular mycorrhizal fungus GZ176 for herbaceous plant disease prevention, growth promotion and stress resistance, as well as its bacterial agent and application. Background technique [0002] China is a large agricultural country. The total production of herbal plants (including rice, wheat, tomato, pepper, eggplant, tobacco, cucumber, watermelon, melon, celery, etc., horticultural flowers such as chrysanthemum, lawn grass, and Chinese herbal medicines such as Sanqi) The area has exceeded 2.2 billion mu, and it has made great contributions to the development of agriculture in the world. However, my country is also a big country with frequent, frequent and heavy occurrences of pests and diseases. Pests and diseases, floods and droughts are also called the three natural disasters in my country. There are more than 1,000 common pests and diseases in my country, and nearly 100 ma...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/04A01P1/00A01P3/00A01P5/00A01P21/00C12R1/645
CPCA01N63/30C12N1/145C12R2001/645
Inventor 陈刘军
Owner 南京本源生态农业科技有限公司
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