Mycorrhizal fungi strain efficiently growing with rhododendron seedling

A technology of mycorrhizal fungi and strains, applied in the field of microbiology, can solve the problems of affecting nutrient absorption of azalea seedlings, slow growth of azalea seedlings, and high mortality, so as to improve the survival rate of seedlings, increase the infection rate of mycorrhizal plants, and promote seedlings. effect of growth

Active Publication Date: 2012-10-03
SHANGHAI ACADEMY OF LANDSCAPE ARCHITECTURE SCI & PLANNING
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  • Abstract
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  • Application Information

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Problems solved by technology

In addition, Rhododendron is an acidic soil plant, and the optimum soil pH value is 4.5~5.5. In the artificial breeding environment, it is difficult to maintain the acidic environment of pH 4.5~5.5 in the cultivation soil. Often the soil pH is too high, wh

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  • Mycorrhizal fungi strain efficiently growing with rhododendron seedling
  • Mycorrhizal fungi strain efficiently growing with rhododendron seedling
  • Mycorrhizal fungi strain efficiently growing with rhododendron seedling

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Example Embodiment

[0013] Example 1 Isolation and identification of rhododendron mycorrhizal fungi:

[0014] 1. Isolate the root system material: Take the living roots of the azalea species'Purple Crane' in Shanghai Binjiang Forest Park, put them in an ice bucket together with soil moisture, and store them in a refrigerator at 4°C until use.

[0015] 2. Separation and identification of culture medium:

[0016] (1) Separation medium: use Martin-Bengal Red Medium (referred to as MA) to adjust and improve its pH. The formula is: 10 grams of glucose, 5 grams of tryptone, 1 gram of dipotassium hydrogen phosphate, 0.5 grams of magnesium sulfate, 0.033 grams of Bengal red, 20 grams of agar, distilled water to a constant volume of 1000 ml, adjust the pH to 5.0, and then high pressure at 121°C Sterilize for 20 minutes, add 0.03 g of streptomycin when cooled to below 60°C, mix well, and pour the plate for use.

[0017] (2) Strain identification medium: use malt extract medium (MEA), the formula is: malt extract ...

Example Embodiment

[0031] Example 2

[0032] The strain of the present invention is isolated from the root system of the azalea species'Zihe' in Shanghai Binjiang Forest Park. It belongs to the genus Oidiodendron OBJF31. It can be cultured on a flat plate or in a liquid. The MEA medium is used. The formula is: Malt The extract is 20g, tryptone 1g, glucose 20g, the pH value of the culture medium is 5.1, and the culture temperature is 22~24℃.

[0033] The macroscopic characteristics of the strain culture are: the colony grows slowly, initially grayish white, after 10 days, the color of the center of the colony becomes darker and brown, the texture of the colony is tapetum-like, round, and the center is slightly raised. The colony diameter is 2.0~2.5 for 14 days of plate culture. cm. Microscopic characteristics: The hyphae are 2.0~3.0μm in diameter, with single-celled conidia, oval, smooth surface, size 2.5~3.5μm*1.5~2.0μm, with conidiophore, length 80~120μm. The closest species in the ITS sequence al...

Example Embodiment

[0037] Example 3

[0038] Rhododendron fortunei seedlings cultured aseptically for 9 weeks, about 2 to 4 cm in height, were transplanted in sterilized substrates with different pH values. The method of adjusting the pH value of the cultivation substrate: Based on the original pH value of the cultivation substrate, add a certain amount of CaCO according to the volume ratio of the substrate 3 (Adding ratio is according to 0, 3, 5kg / m 3 ), adjust the pH value of the cultivation substrate to 5.5, 6.8, 7.2 respectively. The seedlings were inoculated after transplanting, and the inoculated strains were OBJF31 (preservation number: CGMCC No. 6010) and HS04. The inoculation method and seedling culture conditions were the same as those in Example 1. Each treatment was inoculated with 30 plants, and a non-inoculated control was set. After 20 weeks of inoculation, the infection rate and biomass of the seedlings were tested (Table 2). The biomass and survival rate of the inoculated seedlings...

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Abstract

The invention belongs to the field of microbiology and provides a mycorrhizal fungi strain efficiently growing with a rhododendron seedling. The problems of the prior art that the seedling growth is slow, the death rate is high, the leaves are yellow, and the like, during the artificial seedling culturing process for the rhododendron are solved. The preservation number of the mycorrhizal fungi strain provided by the invention is CGMCCNo:6010; the mycorrhizal fungi strain belongs to ascomycetes, chactomium globosum, discellaceae, oidiodendron; the mycorrhizal fungi strain provided by the invention can efficiently grow together with the rhododendron seedling; the rhododendron seedling is inoculated in a seedling stage; the infection rate of mycorrhiza is high; the growth of the seedling is obviously boosted; the survival rate of the seedling is increased; and when the pH of soil is 5.5-7.2, the strain inoculated on the seedling can effectively promote the growth of the seedling.

Description

Technical field: [0001] The invention relates to the field of microbiology, in particular to a fungal strain, in particular to a rhododendron-like mycorrhizal fungal strain, in particular to a mycorrhizal fungal strain that efficiently symbioses with rhododendron seedlings. Background technique: [0002] Rhododendron is one of the top ten traditional famous flowers in my country. It has the reputation of "the beauty of flowers" and has high ornamental value. It has always been loved by people. At the same time, my country is the main distribution center of Rhododendron in the world, with about 70% of Rhododendron species resources distributed in the world, and many precious Rhododendron resources are unique to my country. Rhododendron is an important component of vegetation in many nature reserves, forest tourist attractions or forest parks, forming a characteristic and prominent vegetation landscape, which has important ecological value and tourism development value. Moreo...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/04A01P21/00C12R1/645
Inventor 张春英黄芳尹丽娟陈真杨兵
Owner SHANGHAI ACADEMY OF LANDSCAPE ARCHITECTURE SCI & PLANNING
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