Improved efficient white muscardine silkworm cultivating method

A cultivation method and technology of silkworm, which are applied in the field of improved high-efficiency cultivation of silkworm silkworm, can solve the problems of low infection rate and high rigidity rate of silkworm, and achieve the goal of increasing infection rate, yield of silkworm silkworm, and infection rate of silkworm Effect

Active Publication Date: 2014-11-19
SERICULTURE & AGRI FOOD RES INST GUANGDONG ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the defect of low infection rate in existing silkworm production methods, the object of the present invention is to provide an improved method for efficiently cultivating silkworm silkworms, which preferably obtains several previously unknown silkworm eggs that are susceptible to beauveria bassiana, In addition, more detailed research has been carried out on the existing inoculation period, and the inoculation period with higher infection rate has been found; the method of the present invention has strong operability, high ossification rate of silkworm, and excellent quality of silkworm obtained

Method used

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  • Improved efficient white muscardine silkworm cultivating method

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Experimental program
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Effect test

Embodiment 1

[0019] A method for cultivating a dead silkworm, comprising the following steps:

[0020] (1) Containing 0.2% Tween-80 sterile water to prepare concentrations of 1×10 6 , 1×10 5 , 1×10 4 , 1×10 3 , 1×10 2 Individual / mL Beauveria bassiana spore suspension;

[0021] (2) Raise 18 varieties of silkworms. Two hours after the moulting of the fourth instar silkworms, the silkworms were soaked in the spore suspension of Beauveria bassiana for 10 seconds, then fished out, put into insect breeding boxes and raised, 30 in each treatment group Silkworm, set 3 replicates. Investigate the death situation every day for 7 consecutive days and calculate LD 50 .

[0022] LD of 18 Silkworm Species 50 and mortality at day 7 see figure 1 .

[0023] Depend on figure 1 It can be seen that there are great differences in the tolerance of different varieties to Beauveria bassiana. Anti-8 and limited-9 are the most resistant to Beauveria bassiana, while Haoyue, Taiyuan, Hang 7 and other vari...

Embodiment 2

[0025] A method for cultivating a dead silkworm, comprising the following steps:

[0026] (1) Containing 0.2% Tween-80 in sterile water to prepare a concentration of 1 × 10 3 Individual / mL Beauveria bassiana spore suspension;

[0027] (2) Raising silkworms (Haoyue), 2h, 7h, and 12h after the moulting of the fourth instar silkworm, the silkworms were soaked in the spore suspension of Beauveria bassiana for 10 seconds, then fished out, put into insect breeding boxes and raised, each treatment A group of 30 silkworms was set up with 3 replicates. Investigate the death situation every day for 7 consecutive days and calculate LD 50 .

[0028] Comparison of the mortality rates of silkworms inoculated with Beauveria bassiana at different stages figure 2 .

[0029] Depend on figure 2 It can be seen that after silkworms are inoculated with Beauveria bassiana within 2 hours, the ossified mortality rate of more than 85% can be reached 96 hours after the inoculation, the onset tim...

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Abstract

The invention discloses an improved efficient white muscardine silkworm cultivating method. The method includes the following steps that silkworms are bred, beauveria bassiana spore suspension is sprayed on the surfaces of the silkworms within the 0-2h after the four-instar moulting silkworms molt, the silkworms die due to attack of diseases after five days to six days, the dead silkworms are picked out for further muscardine, and the silkworms are aired or dried to obtain muscardine silkworms. The varieties of the silkworms are Haoyue, Taiyuan and Hang 7. The silkworm varieties poor in tolerance of beauveria bassiana are screened out to serve as the varieties special for white muscardine silkworm production, so that the infection rate of the silkworms is improved. The extreme early stage of the five-instar silkworms newly molting is selected as the optimal period for inoculating the silkworms with the beauveria bassiana, and therefore the infection rate of the silkworms and the yield of the white muscardine silkworms are increased.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicine production, and in particular relates to an improved high-efficiency cultivation method for silkworm Ambrysalis larvae. Background technique [0002] Bombyx Batryticatus is a traditional Chinese medicine. "Chinese Pharmacopoeia" records that Bombyx Batryticatus has the effects of relieving wind and spasm, dispelling wind and relieving pain, resolving phlegm and dispelling stagnation. Cold, apoplexy, headache due to wind-heat, red eyes and sore throat, rubella itching, and mumps. Modern pharmacological experiments have also confirmed that the water extract or alcohol extract of Bombyx mori has significant effects in anticonvulsant, anticoagulant, antibacterial, anticancer, hypoglycemic and lipid-lowering. With the in-depth research on the pharmacological components and functions of the silkworm and the continuous research and development of new products, the application of the silkworm ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/04
Inventor 邢东旭杨琼廖森泰罗国庆肖阳李丽李庆荣叶明强刘新涛
Owner SERICULTURE & AGRI FOOD RES INST GUANGDONG ACAD OF AGRI SCI
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