Improved efficient white muscardine silkworm cultivating method
A cultivation method and technology of silkworm, which are applied in the field of improved high-efficiency cultivation of silkworm silkworm, can solve the problems of low infection rate and high rigidity rate of silkworm, and achieve the goal of increasing infection rate, yield of silkworm silkworm, and infection rate of silkworm Effect
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Embodiment 1
[0019] A method for cultivating a dead silkworm, comprising the following steps:
[0020] (1) Containing 0.2% Tween-80 sterile water to prepare concentrations of 1×10 6 , 1×10 5 , 1×10 4 , 1×10 3 , 1×10 2 Individual / mL Beauveria bassiana spore suspension;
[0021] (2) Raise 18 varieties of silkworms. Two hours after the moulting of the fourth instar silkworms, the silkworms were soaked in the spore suspension of Beauveria bassiana for 10 seconds, then fished out, put into insect breeding boxes and raised, 30 in each treatment group Silkworm, set 3 replicates. Investigate the death situation every day for 7 consecutive days and calculate LD 50 .
[0022] LD of 18 Silkworm Species 50 and mortality at day 7 see figure 1 .
[0023] Depend on figure 1 It can be seen that there are great differences in the tolerance of different varieties to Beauveria bassiana. Anti-8 and limited-9 are the most resistant to Beauveria bassiana, while Haoyue, Taiyuan, Hang 7 and other vari...
Embodiment 2
[0025] A method for cultivating a dead silkworm, comprising the following steps:
[0026] (1) Containing 0.2% Tween-80 in sterile water to prepare a concentration of 1 × 10 3 Individual / mL Beauveria bassiana spore suspension;
[0027] (2) Raising silkworms (Haoyue), 2h, 7h, and 12h after the moulting of the fourth instar silkworm, the silkworms were soaked in the spore suspension of Beauveria bassiana for 10 seconds, then fished out, put into insect breeding boxes and raised, each treatment A group of 30 silkworms was set up with 3 replicates. Investigate the death situation every day for 7 consecutive days and calculate LD 50 .
[0028] Comparison of the mortality rates of silkworms inoculated with Beauveria bassiana at different stages figure 2 .
[0029] Depend on figure 2 It can be seen that after silkworms are inoculated with Beauveria bassiana within 2 hours, the ossified mortality rate of more than 85% can be reached 96 hours after the inoculation, the onset tim...
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