Paecilomyces metabolite and application thereof

A metabolite, Paecilomyces spp. technology, applied to Paecilomyces pyogenes metabolites and their application fields, can solve problems such as lack of sporozoites, and achieve the effect of easy preparation

Inactive Publication Date: 2010-07-28
YUNNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Conidiophores arise from spreading aerial hyp...

Method used

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  • Paecilomyces metabolite and application thereof
  • Paecilomyces metabolite and application thereof
  • Paecilomyces metabolite and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1: The toxic effect of Paecilomyces 1788 fermented liquid to pine xylophilus

[0031] The cultivated liquid fermented product is vacuum filtered to obtain a clear solution, which is concentrated under reduced pressure at 60-70°C to dryness to obtain the active substance, dissolved in sterile distilled water to prepare a 5 mg / ml sample, and tested according to the above test method. effect test. Tables show adjusted mortality rates.

[0032] Table 1 Toxic effect of fermentation broth on B.xylophilus nematode

[0033]

[0034] The results showed that the liquid fermentation product of Paecilomyces 1788 had a significant poisonous effect on pine xylophilus, and the lethal rate reached over 92%.

Embodiment 2

[0035] Example 2: Toxic action of active components on pine xylophilus

[0036] The isolated active ingredient was dissolved in sterile distilled water and diluted to a 500ppm solution.

[0037] The drug efficacy test was carried out according to the above-mentioned test method. Tables show adjusted mortality rates.

[0038] Table 2 Toxicity of active components to B.xylophilus nematodes

[0039]

[0040] The results show that the lethality of the isolated active components to B. xylophilus nematodes reaches over 80% within 24 hours. Has obvious nematicidal activity.

Embodiment 3

[0041] Embodiment 3: the half-lethal rate ED of active component to pine xylophilus 50

[0042] Embodiment 3 method is the same as embodiment 2, and difference is: 2.5mg active component is dissolved in a small amount of sterile distilled water, is mixed with the aqueous solution of 1000ppm, then half-diluted, measure half-lethal rate (ED 50 ) and 95% lethality (ED 95 ).

[0043] Table 3 ED for B. xylophilus nematodes 50 and ED 95

[0044]

[0045] The results showed that the semi-lethal rate (ED) of the active ingredient to B.xylophilus nematode in 24 hours50 ) is 299.5 ppm, 95% lethality (ED 95 ) is 796.2 ppm.

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Abstract

The invention relates to a paecilomyces metabolite and application thereof, which belong to the technical field of microbial medicine screening. The invention is realized by the following steps that: a production strain is paecilomyces sp.1788, and the preservation code is CGMCCNo. 3422; the paecilomyces sp.1788 is fermented and cultured by czapek culture medium conventional liquid, the fermentation fluid is concentrated at low pressure, column chromatography is carried out to water phase repeatedly, and the paecilomyces metabolite of the invention is prepared after elution. The paecilomyces metabolite of the invention has the function of killing bursaphelenchuh xylophilus, the lethal rate (ED50) in 24h is 299.5ppm, and the ED95 is 796.2ppm, and can be applied to bursaphelenchuh xylophilus preparations. Compared with the traditional bursaphelenchuh xylophilus chemical, the invention is characterized by high efficiency, low toxin, water solubility, easy preparation and the like.

Description

Technical field: [0001] The invention relates to a Paecilomyces metabolite and its application, belonging to the technical field of microbial drug screening. Background technique: [0002] Some species of Paecilomyces play an important role in controlling plant pests, such as the control of Paecilomyces lilacinus to cyst nematodes, root-knot nematodes and other plant parasitic nematodes, Paecilomyces fumigatus and Paecilomyces fumigatus It can parasitize and kill a variety of insects, including Homoptera, Lepidoptera, Diptera, Coleoptera, Hymenoptera, etc. In many countries, these Paecilomyces have been successfully developed into biocontrol agents, which play an important role in the construction of ecological agriculture. [0003] Paecilomyces Bainier belongs to the subphylum Deuteromycotina, class Hyphomycetes, order Hyphomycetales, family Moniliaceae. The main morphological feature is multinucleate hyphae, white, off-white to dark white or brown, spreading from hairy. ...

Claims

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Application Information

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IPC IPC(8): C12P1/02A01N63/04A01P5/00C12R1/79
Inventor 刘亚君张克勤刘意
Owner YUNNAN UNIV
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