LAMP primer group and LAMP detection kit for detecting bursaphelenchus xylophilus by taking Bx-tlp-2 as target gene

Abstract

The invention discloses an LAMP primer group and an LAMP detection kit for detecting bursaphelenchus xylophilus by taking Bx-tlp-2 as a target gene. A plurality of pairs of LAMP detection primers are designed by taking the Bx-tlp-2 gene of the bursaphelenchus xylophilus as a target gene for LAMP detection of the bursaphelenchus xylophilus, and the LAMP primer group with high specificity and high detection efficiency is finally screened through a large number of screening tests. On the basis, the invention provides an LAMP (loop-mediated isothermal amplification) detection method and an LAMP kit for detecting bursaphelenchus xylophilus. The LAMP detection method and the detection kit provided by the invention have the advantages of simplicity and convenience in operation, low cost, high stability, strong specificity, high detection efficiency and the like, and have application prospects in pine wood nematode detection.

Description

technical field [0001] The invention relates to a kit for detecting plant nematodes, in particular to a LAMP detection primer for detecting Bursaphelenchus xylophilus, a detection method and a LAMP detection kit, belonging to the detection field of pine wood nematodes. Background technique [0002] Pine wilt disease, also known as pine wilt disease, is a devastating pine forest disease caused by pine wood nematode infestation. Bursaphelenchus xylophilus(Steiner&Buhrer)(Nickle WR,Golden AM,MamiyaY,et al.On the taxonomy and morphology of the pine wood nematode,Bursaphelenchus xylophilus(Steiner&Buhrer1934) Nickle 1970.Journal of Nematology,19385,1970, ) belongs to the linear animal phylum, the order Aphelenchida, the family Aphelenchoididae, and the genus Bursaphelenchus. The resin secretion of pine trees infected with B. xylophilus gradually decreases, and the needles first fade and yellow, then wither and turn reddish brown, and finally the whole plant dies. The onset of p...

AI Technical Summary

Problems solved by technology

[0006] The key to the successful detection of B. xylophilus by using the loop-mediated isothermal amplification technique is to design a LAMP primer set with strong specificity and high sensitivity f

Images