Trehalase gene Bx-tre1 and application thereof

A technology of trehalase and gene, which is applied in the field of trehalase gene Bx-tre1, can solve problems such as the spread of pine wood nematodes, achieve the goal of inhibiting sexual reproduction, reducing the activity of trehalase, and blocking the metabolic pathway of trehalose Effect

Active Publication Date: 2019-09-20
NORTHEAST FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to solve the problem that pine xylophilus larvae in the stress-resistant state can enter the osmotic cryptophytic state and survive in a high osmotic pressure environment, thereby causing the spread of pine wood nematode, and provide a trehalase gene Bx-tre1 and its application

Method used

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  • Trehalase gene Bx-tre1 and application thereof
  • Trehalase gene Bx-tre1 and application thereof
  • Trehalase gene Bx-tre1 and application thereof

Examples

Experimental program
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specific Embodiment approach 1

[0012] Specific embodiment 1: The nucleotide sequence of a trehalase gene Bx-tre1 in this embodiment is shown in the sequence table Seq ID No: 1, with a length of 1413 bp.

[0013] The expression level of the trehalase gene in this embodiment is significantly higher in the male pine xylophilus than in other stages, indicating that the gene plays an important role in the male pine xylophilus. Reducing the expression of this gene in male pine wood nematodes by gene silencing can effectively reduce the enzyme activity of trehalase and block the metabolic pathway of trehalose, thereby reducing the 55%-60 % survival rate, thereby inhibiting sexual reproduction, has important application value in the control of pine wood nematode.

specific Embodiment approach 2

[0014] Specific embodiment two: the difference between this embodiment and specific embodiment one is: the nucleotide sequence of the upstream primer for constructing the trehalase gene Bx-tre1 is shown in the sequence table Seq ID No: 2; the nucleotide sequence of the downstream primer The sequence is shown as Seq ID No:3 in the sequence table. Others are the same as the first embodiment.

specific Embodiment approach 3

[0015] Embodiment 3: In this embodiment, the trehalase gene Bx-tre1 is applied to the prevention and treatment of pine diseases.

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Abstract

The invention relates to trehalase gene Bx-tre1 and application thereof and aims to solve the problem of bursaphelenchus xylophilus spread due to the fact that stress-resistant larvae of bursaphelenchus xylophilus are capable of starting a variable percolation state to survive in a high-permeation-pressure environment. The trehalase gene Bx-tre1 is applied to pine tree disease and pest prevention and control. A nucleotide sequence of the trehalase gene Bx-tre1 is shown as Seq ID NO:1 in a sequence table. By gene silencing, the expression quantity of the trehalase gene in male bursaphelenchus xylophilus can be reduced, trehalase activity can be effectively lowered, a trehalose metabolism path is blocked, and accordingly the survival rate of the male bursaphelenchus xylophilus under high-permeation-pressure stress is remarkably decreased, and sexual propagation is further inhibited. The trehalase gene Bx-tre1 is applied to the field of bursaphelenchus xylophilus prevention and control.

Description

technical field [0001] The invention relates to a trehalase gene Bx-tre1 and its application. Background technique [0002] Pine wood nematode (Bursaphelenchus xylophilus) causes pine wilt disease, which has harmed Japanese, Chinese and Korean pine forests for nearly half a century after it was introduced from North America to East Asia, causing huge economic losses. The nematode has a complex life history and a strong ability to adapt to the environment. It has gradually spread to the cold northern regions. Pine trees in cold regions adapt to low temperature environment. In order to prevent frost cracking due to high water content in the tree body, the metabolism of the tree starts to slow down in autumn, the water content in the tree body drops, and the osmotic pressure rises sharply, which is not conducive to the survival of pine xylophilus. However, sampling surveys of overwintering B. xylophilus in Dalian and Fushun showed that B. xylophilus can survive in a high-osmot...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/56C12N9/24A01N57/16A01P5/00
CPCC12N9/2402A01N57/16C12Y302/01028
Inventor 王峰陈俏丽王佳楠李丹蕾
Owner NORTHEAST FORESTRY UNIVERSITY
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