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Active material extracted from euphorbia lathyris and application thereof

A technology of active substances and saccharides, which is applied in the field of biological pesticides and achieves the effects of low cost and simple preparation method

Inactive Publication Date: 2012-07-25
YUNNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far there is no report on the activity of E.lathyris against B. xylophilus

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Rinse the sub-twigs with tap water and put them into an oven, and bake them for 10 hours at a temperature of 50°C. Cut 10 grams of dried branches into pieces, put them in a triangular flask, extract three times with 80% ethanol, each time is 4, 12 and 24 hours respectively, after combining the extracts of the three extractions, concentrate to The solvent was evaporated to obtain the crude extract of branch. The extract was dissolved in methanol to prepare a concentration of 0.1 g / ml. According to the above-mentioned experimental method, the poisonous activity of the extract with a concentration of 5 mg / ml on pine wood nematodes was done, and the nematode suspension containing methanol of the corresponding concentration and the nematode suspension without the capsicum extract was used as a control. The results are shown in Table 1.

[0019] The lethality (%) of the ethanol extract of 80% of the continuation of the sporocarp to pine xylophilus in table 1

[0020] ...

Embodiment 2

[0023] Basically the same as embodiment one, the difference is that the material used is the leaves of caperia. After washing with tap water, the cotyledons were put into an oven, and baked for 10 hours at a temperature of 50°C. 10 grams of dried leaves are chopped, put into a triangular flask, extracted three times with 80% ethanol, each time is 4, 12 and 24 hours respectively, after combining the extracts of the three extractions, concentrate to A crude extract of leaves was obtained after evaporation of the solvent. The extract was dissolved in methanol to prepare a concentration of 0.1 g / ml. According to the above-mentioned experimental method, the poisonous activity of the extract with a concentration of 5 mg / ml was performed on pine wood nematodes, and the nematode suspension containing methanol of the corresponding concentration and the nematode suspension without the capsicum extract was used as a control. The results are shown in Table 2.

[0024] The lethality (%) ...

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PUM

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Abstract

The invention relates to an active material extracted from euphorbia lathyris and application thereof, and belongs to the technical field of biological pesticides. The active material extracted from euphorbia lathyris is characterized in that a method for obtaining the active material comprises the following steps of: a, cleaning collected branches or leaves of the euphorbia lathyris by tap water, putting the cleaned branches or leaves in an oven for roasting at the temperature of 50 DEG C for 10 hours, cutting 10g of dried branches or leaves into pieces, and putting the cut branches or leaves in a triangular flask, soaking the branches or leaves by 80 percent ethanol, wherein the time for the first soaking extraction is 4 hours, and the time for the second and the third soaking extractions is 12 hours and 24 hours respectively; b, mixing extraction solution obtained by the extractions at the third time, condensing the obtained solution until the solvent is vaporized to obtain coarse extracts of the branches or leaves, dissolving the coarse extracts in methanol to prepare the solution of which the concentration is 0.1g / ml, and putting the obtained solution in a refrigerator for later use. The active material can be used for preparing a biological reagent of killing B.xylophilus. The active material has the advantages of low cost, simple and convenient preparation method, high efficiency and low toxicity, which can be used for preparing the biological reagent of killing B.xylophilus.

Description

Technical field: [0001] The invention relates to an active substance extracted from caper caper and its application, and belongs to the technical field of biopesticides. Background technique: [0002] The pine wood nematode (Bursaphelenchus xylophilus) belongs to the class Nematoda, the order Tylenchida, the family Aphelenchoididae, and the genus Bursaphelenchus. Pine xylophilus disease caused by pine wood nematode is a devastating epidemic disease of pine trees. Existing control methods for pine wood nematode disease include chemical control, disease-resistant breeding, control of vector insects, beetles, and treatment of diseased and dead wood, but the effects are not obvious or have their limitations. Natural remedies for wood nematodes. Plants are one of the main sources of active natural products, and finding natural metabolites from plants that are toxic to B. xylophilus has become one of the focuses of nematode biological control research. [0003] Euphorbia lathyr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N65/18A01P5/00
Inventor 李国红张克勤
Owner YUNNAN UNIV
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