A kind of biopesticide for controlling pine wood nematode and preparation method thereof

A technology for biological pesticides, pine wood nematodes, applied in the directions of botanical equipment and methods, chemicals for biological control, biocides, etc., to achieve the effect of wide application prospects

Active Publication Date: 2020-08-18
NORTHEAST FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most studies on biological control of Trichoderma virens (Trichoderma virens) T43 focus on antagonism, antibacterial components, and antibacterial mechanisms. The preparation method of bacterium agent, find no report relevant to the present invention through searching

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] 1. Preparation of PDA Medium

[0028] Follow the modified PDA medium preparation method. Recipe: 200g potato, 20g glucose, 1.5g magnesium sulfate, 3g sodium dihydrogen phosphate, 20g agar, 1000mL water, make 1L liquid. Packed in 500mL Erlenmeyer flasks, about 250mL per bottle. Put the Erlenmeyer flask containing the culture medium into an autoclave (121° C.) for 20 minutes to sterilize.

[0029] 2. Vaccination

[0030] Trichoderma virens T43 was inoculated on PDA medium. First, sterilize the ultra-clean workbench by ultraviolet light for 30 minutes. Pour liquid PDA into each glass Petri dish in a clean bench. After cooling, Trichoderma virens T43 was taken out, inoculated into PDA medium, and placed in a constant temperature incubator at 25°C for several days.

[0031] 3. Preparation of PD Medium

[0032] Prepare PD liquid medium according to the modified PD medium preparation method. Recipe: 200g potatoes, 20g glucose, 1.5g magnesium sulfate, 3g sodium dihydrog...

Embodiment 2

[0044] 1. Preparation of PDA Medium

[0045] Follow the modified PDA medium preparation method. Recipe: 200g potato, 20g glucose, 1.5g magnesium sulfate, 3g sodium dihydrogen phosphate, 20g agar, 1000mL water, make 1L liquid. Packed in 500mL Erlenmeyer flasks, about 250mL per bottle. Put the Erlenmeyer flask containing the culture medium into an autoclave (121° C.) for 20 minutes to sterilize.

[0046] 2. Vaccination

[0047] Trichoderma virens T43 was inoculated on PDA medium. First, sterilize the ultra-clean workbench by ultraviolet light for 30 minutes. Pour liquid PDA into each glass Petri dish in a clean bench. After cooling, Trichoderma virens T43 was taken out, inoculated into PDA medium, and placed in a constant temperature incubator at 25°C for several days.

[0048] 3. Preparation of PD Medium

[0049] Prepare PD liquid medium according to the modified PD medium preparation method. Recipe: 200g potatoes, 20g glucose, 1.5g magnesium sulfate, 3g sodium dihydrog...

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PUM

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Abstract

The invention relates to a biological pesticide capable of preventing and treating bursaphelenchus xylophilus and a preparation method thereof as well as application of the biological pesticide to prevention and treatment of the bursaphelenchus xylophilus. A dosage form of the pesticide is powder; an active component of the powder is Trichoderma virens T43 fermented liquid n-butanol extract; the preparation method of the powder comprises the following steps: filtering and centrifuging fermented liquid, taking supernatant liquid, adding n-butanol, mixing and extracting, removing a water layer,and evaporating and concentrating an n-butanol layer to obtain the powder.

Description

technical field [0001] The invention relates to a biological pesticide for preventing and controlling pine wood nematode, which is extracted from the antibacterial metabolites in the fermentation liquid of Trichoderma virens (Trichoderma virens) T43, a biocontrol fungus that inhibits pine wood nematode, and also relates to a preparation method thereof, especially The invention relates to a liquid fermentation process of Trichoderma virens (Trichoderma virens) T43 and a method for separating and extracting active components, as well as its application in the prevention and control of pine wood nematodes. Background technique [0002] Pine wood nematode (Bursaphelenchus xylophilus) is a devastating disease of pine trees. The mortality rate of trees is extremely high after disease, and it is difficult to prevent and control. It can cause quite serious economic losses and very serious ecological disasters. It has great influence on forestry economy and natural landscape. It is a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N63/38A01N25/12A01P5/00
CPCA01N25/12A01N63/30
Inventor 宋瑞清张壤心逯昕明梁英辉宋倩魏珊珊
Owner NORTHEAST FORESTRY UNIVERSITY
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