Epiphyte Xylaria sp.FDYS-1, biological agent prepared from the same and application thereof in preventing and curing bursaphenchus xylophilus nickle

A technology of biological agents and fungi, which is applied in the field of prevention and control of pine wood nematodes, to achieve the effect of small impact on the ecological environment, high lethal activity, and safety for humans and animals

Inactive Publication Date: 2010-07-14
ZHONGKAI UNIV OF AGRI & ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Shijunzi is a traditional insecticidal medicinal plant, which can produce insecticidal ingredients. Based on the viewpoint that plant endophytic fungi can produce the same or similar active ingredients as their hosts, if the endophytic fungus of Lijunzi plant is the screening object, it should be Strains with toxic and killing activity against pine xylophilus can be obtained, thus laying the foundation for the biological control of nematodes, but there is no relevant report yet

Method used

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  • Epiphyte Xylaria sp.FDYS-1, biological agent prepared from the same and application thereof in preventing and curing bursaphenchus xylophilus nickle
  • Epiphyte Xylaria sp.FDYS-1, biological agent prepared from the same and application thereof in preventing and curing bursaphenchus xylophilus nickle
  • Epiphyte Xylaria sp.FDYS-1, biological agent prepared from the same and application thereof in preventing and curing bursaphenchus xylophilus nickle

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 Isolation of Xyalria sp.FDYS-1 and its activity against pine xylophilus

[0031] Collect the healthy leaves of the Gentleman plant in Guangzhou South China Botanical Garden, rinse them with tap water, dry the water, cut them into small pieces of about 1.5×1.5cm, and carry out surface disinfection. The specific steps are as follows: rinse with 70% alcohol for 30s, and then rinse with 0.1 Rinse with %mercuric chloride for 1min, then rinse with 70% alcohol for 30s, rinse with sterile water for 4 times, and blot dry on sterile filter paper.

[0032] Use the rinsing liquid test method and the tissue imprinting method to test the surface disinfection effect: the rinsing liquid test method is to draw 0.2 mL of the rinsing liquid from the last rinse with sterile water and spread it on the PDA plate; the tissue imprinting method is to use sterilized tweezers to The above-mentioned surface-sterilized materials were transplanted onto the PDA plate and removed after conta...

Embodiment 2

[0037] Example 2 The influence of culture conditions on the production of Xylaria sp.FDYS-1 active substances

[0038] 1. Selection of culture medium

[0039] Inoculate Xylaria sp.FDYS-1 into potato dextrose medium (PD), potato carrot medium (PC), potato sucrose medium (PS), Martin's medium, Czapek medium, Czapek containing 20% ​​potato juice The culture medium and improved Fries No. 3 medium were cultured in 7 kinds of liquid medium including 120r / min shaking at 25°C in the dark for 5 days. The culture filtrate was prepared and the lethal activity of the filtrate against B. xylophilus was tested by the contact method.

[0040] Conclusion: if image 3 As shown, Xylaria sp.FDYS-1 can grow and produce nematocidal active substances in 7 kinds of liquid media, but the culture filtrates obtained from different media have obvious differences in nematocidal activity, and the culture filtrates of PD and PS The nematicide activity of the strains was the strongest, reaching 95.67% and...

Embodiment 3

[0048] Example 3 Genetic Stability of Xylaria sp.FDYS-1 Produced Nematode Active Substance and Thermal Stability of Active Substance

[0049] Xylaria sp.FDYS-1 was continuously subcultured for 6 generations, and then the culture filtrate was prepared and its lethal activity against pine xylophilus was detected.

[0050] Conclusion: by Image 6 As shown, after continuous subculture of Xylaria sp.FDYS-1 for 6 generations, the nematicidal activity of the culture filtrate remained basically unchanged, and the lethal rate against pine xylophilus was still as high as 90.43%.

[0051] Treat the culture filtrate of Xylaria sp.FDYS-1 in a water bath at 60, 70, 80, 90, and 100°C for 20, 30, 40, 50, 60 minutes and autoclave at 121°C for 20 minutes, and use sterile distilled water to make up the lost water , to detect line killing activity. The test results showed that the nematicidal activity of the culture filtrate of the strain Xylaria sp.FDYS-1 had better thermal stability, and the ...

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Abstract

The invention discloses a fungi Xylaria sp.FDYS-1, biological agents prepared by the fungi Xylaria sp.FDYS-1 and application of the fungi Xylaria sp.FDYS-1 in prevention and cure of pine wood nematodes. The fungi Xylaria sp.FDYS-1 is preserved in China Center for Type Culture Collection on February 25th, 2008 and the preservation No. is CCTCC NO:M 208025. The invention inoculates the Xylaria sp.FDYS-1 into a potato glucose liquid medium or a potato sugar liquid medium; the pH of the liquid medium is 5 to 8; the culture temperature is 26 DEG C to 29 DEG C; after cultivation of 3 to 5 days under nonluminous conditions, the culture medium is centrifugally filtered, and the biological agents are obtained by collection of filtrate. The biological agents have high killing activity on the pine wood nematodes, good thermal stability, environment-friendly source without toxity, strong selectivity and small ecological environmental influence. The fungi Xylaria sp.FDYS-1 has low culture condition requirements, basically maintains stable inheritance of the capacity for generation of active materials, and has good development and application prospect.

Description

technical field [0001] The invention belongs to the field of biological pesticides, and in particular relates to a fungus Xylaria sp. FDYS-1 and its prepared biological preparation and its application in preventing and treating pine wood nematode. Background technique [0002] Pine wood nematode is a devastating disease of pine trees. The disease is usually violent and the death rate of infected pine trees is extremely high. It has been called "cancer of the forest" and "forest fire without smoke". Since the first discovery of the disease in Sun Yat-sen Mausoleum in Nanjing in 1982 in my country, it has now spread to 113 counties (districts) in 12 provinces including Jiangsu, Zhejiang, Anhui, Shandong, Hubei, Guangdong, Jiangxi, Chongqing, and Guizhou, with an occurrence area of ​​more than 73hm 2 , 2,000,000 dead pine trees, resulting in huge loss of forestry economy, forest ecology and serious damage to natural landscapes, and pose a serious threat to the pine forests in ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01N63/04A01P5/00C12R1/645
Inventor 向梅梅姜子德袁永平
Owner ZHONGKAI UNIV OF AGRI & ENG
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