Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Technique for producing swainsonine by fermentation of white muscardine fungi

A technology of swainsonine and Beauveria bassiana, applied in the directions of fermentation, microorganism-based methods, microorganisms, etc., can solve problems such as difficulty in reproducing costs, and achieve the effects of convenient sources, reduced production costs, and favorable industrial mass production.

Inactive Publication Date: 2008-06-18
杨凌天力生物技术有限公司
View PDF3 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The invention provides a process for producing swainsonine by fermentation of Beauveria bassiana to overcome the problems of difficult reproduction and high cost in the prior art

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] 1. Biological fermentation of Beauveria bassiana:

[0049] Beauveria bassiana was inoculated in 2L bio-fermentation modified Kjeldahl synthesis No. 1 medium under aseptic condition, cultured with aeration at 25°C for 14 days, and 130 g of mycelium was collected, dried, and set aside.

[0050] The prescription of described improved Kjeldahl synthetic medium No. 1 consists of: soluble starch 10g, dextrin 10g, potassium nitrate 2g, dipotassium hydrogen phosphate 2g, sodium chloride 2g, magnesium sulfate 0.05g, calcium carbonate, ferrous sulfate 0.01 g, casein 0.3g, DL-lysine 0.1g, locoweed water extract 5g, water 1000ml, and add 2.5g of antifoaming mineral oil.

[0051] 2. Extraction of swainsonine in mycelium:

[0052] ①Extraction of total mycelium extract: Accurately weigh 130g of mycelium, put it into 1250mL of 70% ethanol solution, treat it with 20KHz ultrasonic wave at room temperature for 4 times, each time for 1 hour, collect the ethanol extracts and combine them. ...

Embodiment 2

[0057] 1. Biological fermentation of Beauveria bassiana:

[0058] Beauveria bassiana was inoculated in 5L biofermentation-use modified Kjeldahl synthetic No. 1 medium under aseptic condition, cultured with aeration at 23°C for 12 days, and 328g of mycelium was collected, dried, and set aside.

[0059] The formula of described improved Kjeldahl synthetic No. 1 medium consists of: soluble starch 10g, dextrin 10g, potassium nitrate 2g, dipotassium hydrogen phosphate 2g, sodium chloride 2g, magnesium sulfate 0.05g, calcium carbonate 0.02g, sulfurous acid Iron 0.01g, casein 0.3g, DL-lysine 0.1g, locoweed water extract 5g, water 1000ml, and add 2g antifoaming mineral oil.

[0060] 2. Extraction of swainsonine in mycelium:

[0061] ①Extraction of total mycelium extract: Accurately weigh 328g of mycelium, put it into 3000mL of 70% ethanol solution, treat it with 20KHz ultrasonic wave at room temperature for 4 times, each time for 0.5 hours, collect the ethanol extracts and combine th...

Embodiment 3

[0066] 1. Biological fermentation of Beauveria bassiana:

[0067] Beauveria bassiana was inoculated in 10L bio-fermentation-use modified Kjeldahl synthetic No. 1 medium under aseptic condition, cultured with aeration at 20°C for 10 days, and 675g of mycelium was collected, dried, and set aside.

[0068] The formula of described improved Kjeldahl synthetic No. 1 medium consists of: soluble starch 10g, dextrin 10g, potassium nitrate 2g, dipotassium hydrogen phosphate 2g, sodium chloride 2g, magnesium sulfate 0.05g, calcium carbonate 0.02g, sulfurous acid Iron 0.01g, casein 0.3g, DL-lysine 0.1g, locoweed water extract 5g, water 1000ml, and add 1.5g of defoaming mineral oil.

[0069] 2. Extraction of swainsonine in mycelium:

[0070] ①Extraction of total mycelium extract: Accurately weigh 675g of mycelium, put it into 7000mL of 70% ethanol alcohol solution, treat it with 20KHz ultrasonic wave at room temperature for 4 times, each time for 0.5 hours, collect the ethanol extracts a...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the fermentation engineering technical field, in particular relates to the technics of producing swainsonine fermented by beauveria. The invention provides the technics of producing the swainsonine fermented by the beauveria to overcome the problems of the hard reproduction and the high cost of the prior art. In order to overcome the problems of the prior art, the technical scheme adopted by the invention is the process of producing the swainsonine fermented by the beauveria. The process successively includes the following steps: first, mycelium is obtainedthe through biological fermentation of the beauveria; second, the extraction of the swainsonine in the mycelium: firstly, the extraction of the total extract of the mycelium: the myceilium extraction concentrated solution is obtained by a ultrasonic method; secondly, the extraction of swainsonine crude products: the swainsonine crude products can be obtained by repeated extraction; thirdly, the extraction of the pure swainsonine: the pure swainsonine is obtained by the sublimation technology.

Description

Technical field: [0001] The invention belongs to the technical field of fermentation engineering, in particular to a process for producing swainsonine by fermentation of Beauveria bassiana. Background technique: [0002] The anti-tumor activity, immunoenhancing effect and anti-radiation effect of swainsonine are very obvious and cannot be questioned. However, the key to restricting the research and development of swainsonine as an anti-tumor drug, the industrialization of the product, and the final clinical application is that the source of pure swainsonine is very scarce, which is far from meeting the needs of people's research. There are currently three sources of swainsonine, (1) extracting and separating swainsonine from plants: the content of swainsonine in plants is very low, only about 0.02%, and plant resources are less affected by natural factors. Large, excessive harvesting will cause damage to grassland vegetation, desertification and degradation of grassland, wh...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12P17/06C12N1/14C12R1/645
Inventor 赵宝玉来航线贺武利刘彬慧
Owner 杨凌天力生物技术有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com