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Matrine and beauveria brongniartii combined insecticide and application thereof

A technology of Beauveria bassiana and insecticide, applied in the application, insecticide, biocide and other directions, can solve the problems of resistance and insignificant efficacy, achieve good control effect, no environmental pollution, and reduce the use of amount of effect

Active Publication Date: 2018-10-12
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The present invention finds through research on the compounding effect of matrine and Beauveria bassiana that the compounded insecticides can show significant synergistic effect, improve the control effect, and can significantly reduce the cost of a single dose of chemical pesticides. Use dosage, reduce environmental pollution, and effectively solve the problems of existing pesticides that are prone to resistance and ineffective effects

Method used

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  • Matrine and beauveria brongniartii combined insecticide and application thereof
  • Matrine and beauveria brongniartii combined insecticide and application thereof
  • Matrine and beauveria brongniartii combined insecticide and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Example 1 Screening and identification of Beauveria bassiana SB010

[0045] 1. Collect and isolate Beauveria bassiana

[0046] Beauveria bassiana strain SB010 was originally isolated from soil and is a native strain in China.

[0047]Soil samples were collected. When sampling, about 100 g of soil at 10-15 cm below the topsoil was taken, sealed in plastic bags and brought back to the laboratory for processing. Soil samples were sieved to remove stones and sundries, then 10 g of pure soil was suspended in 90 mL of 0.1% Tween 80 solution, shaken well, and left to stand for 15 min, then 2 mL of the supernatant was diluted in 8 mL of 0.05% Tween 80 Tween 80, prepared as a soil suspension. Inoculate 0.1 mL of soil suspension on the Bengal red agar medium plate, push the suspension evenly under the surface of the plate with a triangular glass scraper, incubate at 25°C for 3-7 days, then cut out a single colony with an inoculation loop, and inoculate it on PDA Continue cultu...

Embodiment 2

[0067] Example 2 A kind of insecticide used in combination with matrine water agent and Beauveria bassiana

[0068] 1. Experimental materials

[0069] The matrine used in the present invention is 0.5% matrine aqueous solution, which is provided by Guangdong Xinjing Biological Engineering Co., Ltd.

[0070] The initial concentration of the Beauveria bassiana SB010 bacterial strain spore suspension that the present invention uses is 1 * 10 9 Conidia / mL, diluted to a lower concentration by stepwise dilution method for experiment. The different concentrations and combinations of Beauveria bassiana and matrine used in experiments in each group are shown in Table 1.

[0071] Table 1 Different concentrations and combinations of Beauveria bassiana and matrine used in the experiment

[0072]

[0073] Note: Mt=matrine (Matrine), Bb=beauveria bassiana ( Beauveria brongniartii ); CT1 (1:9), CT2 (2:8) represent the toxicity test of matrine and Beauveria brucella in different volume ...

Embodiment 3

[0081] Example 3 Indoor Toxicity Determination of Compound to Spodoptera litura

[0082] 1. Experimental method

[0083] (1) During the indoor toxicity test, matrine and Beauveria bassiana were formulated to the required concentration according to the method of Example 1, and 5 concentration gradients were set for each treatment, and 3 repetitions were set for each concentration, and each repetition was 20 headworm.

[0084] (2) First cut the feed of Spodoptera litura into small cubes of 1 cm×1 cm, soak it in the liquid for about 5 s, take it out, absorb the excess liquid and put it into a small box connected with the second instar larvae of Spodoptera litura, As for feeding in the breeding room. The feeding conditions are temperature 25-28 ℃, RH 65%-75%, light as L:D=14 h:10 h.

[0085] (3) Change the feed every two days and clean the small box, observe and record the survival of Spodoptera litura within 7 days after the medicine, and calculate the corrected mortality rate...

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Abstract

The invention discloses a matrine and beauveria brongniartii combined insecticide and application thereof. According to the matrine and beauveria brongniartii combined insecticide, a beauveria brongniartii strain SB010 is preserved in the Guangdong culture collection center on nineteenth April, 2018, and the preservation number of the beauveria brongniartii strain SB010 is GDMCC NO:60359. The insecticide is prepared from main active components of matrine and the beauveria brongniartii. According to the matrine and beauveria brongniartii combined insecticide and application thereof, research finds that a combination of the matrine and the beauveria brongniartii has a significant synergistic interaction effect, the usages of the matrine and the beauveria brongniartii can be significantly reduced, the use cost is reduced, the insecticide is environmentally friendly, no drug resistance is generated, the insecticide has very high application potential for biological control of prodenia litura, the environmental negative effect problem brought by long-term use of chemical pesticides is solved, moreover, the insecticide has the advantages of being green, environmentally friendly, pollution-free and long in lasting time, and thus the application prospects are very good.

Description

technical field [0001] The invention belongs to the technical field of biological control. More specifically, it relates to an insecticide combined with matrine and Beauveria bassiana and its application. Background technique [0002] Beauveria bassiana ( Beauveria brongniartii ) is an entomopathogenic fungus. As a living biological pesticide, it has a mechanism of action different from that of existing chemical insecticides, and has the characteristics of no pollution to the environment and no residue. Control alternatives. [0003] Spodoptera litura ( Spodoptera litura (Fabricius)) is an important agricultural pest distributed worldwide, mainly harming cotton, tobacco, soybean, taro, peanut, sesame, lotus root and cruciferous vegetables, etc., and has the characteristics of intermittent outbreaks. In recent years, with the change of my country's planting industry structure, the vegetable planting area has continued to expand, coupled with climate warming, the occurren...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/04A01N43/90A01P7/04
CPCA01N43/90A01N63/30
Inventor 阿里·肖卡特邱宝利王兴民吴建辉桑文余昕彤
Owner SOUTH CHINA AGRI UNIV
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