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Culture medium for culturing beauveria brongniartii and culture method thereof

A technology of Beauveria bassiana and culture medium, which is applied in the field of culture medium for cultivating Beauveria bassiana, can solve the problems of lack of high-tech industrial production, no mature Beauveria bassiana insecticide products, etc., and achieve the reduction of fungal The total cost of pesticide production, shortening the production cycle, and increasing the effect of trace elements

Inactive Publication Date: 2018-07-10
ANHUI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Mass cultivation of fungi is a prerequisite for field application, but so far there is no mature Beauveria bassiana insecticide product, and there is also a lack of high-tech industrial production

Method used

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  • Culture medium for culturing beauveria brongniartii and culture method thereof
  • Culture medium for culturing beauveria brongniartii and culture method thereof
  • Culture medium for culturing beauveria brongniartii and culture method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Embodiment 1: the solid phase culture medium component is rice preparation method

[0021] 1) Preparation of seed liquid of Beauveria bassiana solid-phase fermentation medium.

[0022] Use SDY+2% maltose medium to prepare Beauveria bassiana solid-phase fermentation medium seed liquid; the method is to inoculate the activated Beauveria bassiana spores evenly in 500mL with a sterile inoculation needle on the ultra-clean workbench Contain SDY+2% maltose medium liquid medium in the conical flask, place it in a constant temperature shaker, the conditions are temperature 25±1°C, 170rpm for 96h, until the medium becomes viscous and the wall of the conical flask White sticky matter appeared, that is, the seed liquid of Beauveria bassiana solid-phase fermentation medium was obtained.

[0023] 2) Solid-phase fermentation culture

[0024] Use an electronic balance to accurately weigh 1000g of cheap rice purchased in the market, and place it in an iron basin with a diameter equa...

Embodiment 2

[0027] Embodiment 2: the solid phase culture medium component is The mass ratio of rice flour + peanut shell is 1:1 preparation method

[0028] The preparation method is the same as in Example 1, step 2) solid-phase fermentation culture is as follows: use an electronic balance to accurately weigh 500 g of cheap rice and peanut shells purchased in the market, use a small grinder to grind the rice into rice flour, and grind the peanuts into near-sheets shape. Dissolve the crushed rice flour in water to make a uniform rice flour solution and distribute it evenly on the peanut shells soaked in water, and place it in an iron basin with a diameter equal to 40cm, seal it with gauze, and then place it in a sterilizing pot at 121°C Autoclave for 20 minutes, and use it as a solid-phase medium after cooling.

Embodiment 3

[0029] Embodiment 3: the solid phase culture medium component is The mass ratio of rice noodles + peanut shells is 2:3 preparation method

[0030] Use the electronic balance to accurately weigh 400g of cheap rice and 600g of peanut shells purchased in the market respectively, use the method of Example 2 to prepare a homogeneous mixture of rice flour and peanut shells, and use it as a solid-phase medium for use after sterilizing and cooling.

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Abstract

The invention discloses a culture medium for beauveria brongniartii and a culture method. The culture medium is used for improving the sporulation quantity of the beauveria brongniartii and the virulence, to an insect, of the beauveria brongniartii. The culture medium is prepared from the following components with component contents: peanut shell, rice flour, wheat bran and eupolyphaga slough, which are in a mass ratio of (1 to 5) to (0.5 to 1.5) to (2 to 6) to (1 to 3). The culture method comprises the following steps of 1), preparation of seed liquid of a solid-phase fermentation culture medium for the beauveria brongniartii; 2), solid-phase fermentation culture; 3), inoculation and culture. The culture medium adopted by the invention can be used for reducing the culture cost, can be also used for increasing a spore yield, is used for shortening a production period, and further, can be used for improving the virulence, to the insect, of a spore.

Description

technical field [0001] The invention relates to the technical field of microbial culture, in particular to a culture medium for cultivating Beauveria bassiana and a culture method thereof. Background technique [0002] Beauveria bassiana, also known as Beauveria bassiana, is an important entomopathogenic fungus that can parasitize more than 70 kinds of insects from 7 orders. Over the years, my country has used Beauveria bassiana to control agricultural and forestry important insects, such as masson pine caterpillars, weevils, longhorns, etc., and good technical results have been obtained. Studies have shown that in the field per square meter of soil at 10 10 Spore treatment can cause pest epidemics in the next year, and the persistence of the fungus in the dead insects and soil ensures the infection of the next generation of pests, thereby maintaining its specific effect in the field. At present, it is the preferred biological agent to control underground pests and develop...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12R1/645
CPCC12N1/14C12N1/145C12R2001/645
Inventor 李茂业潘月敏刘明科
Owner ANHUI AGRICULTURAL UNIVERSITY
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