Culture medium and method capable of increasing Beauveria brongniartii sporulation quantity favorably

Abstract

The invention discloses a culture medium and method capable of increasing Beauveria brongniartii sporulation quantity favorably. The culture medium includes a solid culture medium and a liquid culture medium. The liquid culture medium comprises 3-5mg / l of glucose, 1-5mg / l of peptone and 1-5mg / l of yeast powder, wherein dissolving solvent is water. The solid culture medium comprises yellow mealworm dung, rice husk and rice, wherein the mass ratio of the yellow mealworm dung to the rice husk to the rice is 0.5:1:10. The culture method includes: evenly inoculating fungal spores into the liquid culture medium, placing into a 1000ml triangular flask, performing shake culture for 96 hours at 25 DEG C and 180r / min to obtain seed culture fluid, inoculating the seed culture fluid into the solid culture medium, placing the culture medium into a stainless steel tray and culturing for 3-10 days. The culture medium and method has the advantages that the waste yellow mealworm dung and rice husk are turned into wealth, culture cost is lowered, spore yield is increased, and production cycle is shortened.

Description

technical field

[0001] The invention relates to the technical field of microorganism culture, in particular to a culture medium and a culture method for improving the spore production of Beauveria bassiana. Background technique

[0002] Beauveria bassiana, also known as Beauveria bassiana, is an important entomopathogenic fungus that can parasitize more than 70 species of insects from 7 orders. Over the years, my country has used Beauveria bassiana to control agricultural and forestry important insects such as masson pine caterpillars, weevils, longhorns, etc., and has achieved good technical results. Studies have shown that in the field per square meter of soil at 10 10 Spore treatment can cause pest epidemics in the next year, and the persistence of the fungus in the carcasses and soil ensures the infection of the first generation of pests, thereby maintaining its specific effect in the field. It is the preferred biological agent for controlling underground pests and dev...