Culture medium and method capable of increasing Beauveria brongniartii sporulation quantity favorably

A kind of Beauveria bassiana, culture method technology

Inactive Publication Date: 2015-11-25
CHENGDU YICHUANGSI BIOLOGICAL SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Mass cultivation of fungi is a prerequisite for field application, but so far there is no mature B

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] In the present embodiment, a kind of culture medium that is conducive to improving the spore production of Beauveria bassiana comprises solid medium and liquid medium, and the components and component contents of said liquid medium are as follows: glucose 3mg / l, peptone 1mg / l, yeast powder 1mg / l, the dissolving solvent is water, the composition and mass ratio of the solid medium is: Tenebrio molitor manure: rice husk: rice is 0.5:1:10, and the solvent is water. Inoculate the fungal spores evenly in the liquid medium, place them in a 1000ml Erlenmeyer flask, and culture them on a shaker at 25°C and 180r / min for 96 hours to obtain a seed culture solution, inoculate the seed culture solution into a solid medium, and inoculate the medium Placed in stainless steel trays, cultured for 8 days, placed in a dark environment for the first three days of cultivation, and placed in light conditions for five days after cultivation.

Embodiment 2

[0022] In the present embodiment, a kind of medium that is conducive to improving the spore production of Beauveria bassiana comprises solid medium and liquid medium, and the components and component contents of said liquid medium are as follows: glucose 4mg / l, peptone 2mg / l, yeast powder 2mg / l, the dissolving solvent is water, the composition and mass ratio of the solid medium, Tenebrio molitor manure: rice husk: rice is 0.5:1:10, and the fungal spores are evenly inoculated in the liquid culture Place in a 1000ml Erlenmeyer flask at a temperature of 25°C and culture on a shaker at 180r / min for 96 hours to obtain a seed culture solution, inoculate the seed culture solution into a solid medium, place the medium in a stainless steel tray, and cultivate for 10 days. They were placed in a dark environment three days before culturing, and placed under light conditions for 7 days after culturing.

Embodiment 3

[0024] In the present embodiment, a kind of culture medium that is conducive to improving the spore production of Beauveria bassiana comprises a solid medium and a liquid medium, and the components and component contents of the liquid medium are as follows: glucose 5 mg / l, peptone 4mg / l, yeast powder 2mg / l, the dissolving solvent is water, the composition and mass ratio of the solid medium is: Tenebrio molitor manure: rice husk: rice is 0.5:1:10, and the fungal spores are evenly inoculated in the liquid Place in a 1000ml Erlenmeyer flask in the medium, temperature 25°C, 180r / min, and shake for 96 hours to obtain the seed culture solution. Inoculate the seed culture solution into the solid medium with a liquid-solid inoculation ratio of 0.5:10. Substrates were placed in stainless steel trays and cultured for 3 days.

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PUM

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Abstract

The invention discloses a culture medium and method capable of increasing Beauveria brongniartii sporulation quantity favorably. The culture medium includes a solid culture medium and a liquid culture medium. The liquid culture medium comprises 3-5mg/l of glucose, 1-5mg/l of peptone and 1-5mg/l of yeast powder, wherein dissolving solvent is water. The solid culture medium comprises yellow mealworm dung, rice husk and rice, wherein the mass ratio of the yellow mealworm dung to the rice husk to the rice is 0.5:1:10. The culture method includes: evenly inoculating fungal spores into the liquid culture medium, placing into a 1000ml triangular flask, performing shake culture for 96 hours at 25 DEG C and 180r/min to obtain seed culture fluid, inoculating the seed culture fluid into the solid culture medium, placing the culture medium into a stainless steel tray and culturing for 3-10 days. The culture medium and method has the advantages that the waste yellow mealworm dung and rice husk are turned into wealth, culture cost is lowered, spore yield is increased, and production cycle is shortened.

Description

technical field [0001] The invention relates to the technical field of microorganism culture, in particular to a culture medium and a culture method for improving the spore production of Beauveria bassiana. Background technique [0002] Beauveria bassiana, also known as Beauveria bassiana, is an important entomopathogenic fungus that can parasitize more than 70 species of insects from 7 orders. Over the years, my country has used Beauveria bassiana to control agricultural and forestry important insects such as masson pine caterpillars, weevils, longhorns, etc., and has achieved good technical results. Studies have shown that in the field per square meter of soil at 10 10 Spore treatment can cause pest epidemics in the next year, and the persistence of the fungus in the carcasses and soil ensures the infection of the first generation of pests, thereby maintaining its specific effect in the field. It is the preferred biological agent for controlling underground pests and dev...

Claims

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Application Information

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IPC IPC(8): C12N3/00C12R1/645
Inventor 周洁
Owner CHENGDU YICHUANGSI BIOLOGICAL SCI & TECH
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