Application and method for controlling asparagus prodenia litura by utilizing beauveria bassiana

A technology of Spodoptera litura and Beauveria bassiana, applied in the direction of botany equipment and methods, applications, pesticides, etc., can solve the problems of human and animal hazards, increased difficulty of prevention and control, and impact on the environment, so as to achieve reduced use and long-lasting effect Sexual growth, the effect of preventing and controlling environmental pollution

Inactive Publication Date: 2016-06-15
JIANGXI TIANREN BIOLOGICAL HLDG CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to investigations, Spodoptera litura occurs heavily in asparagus fields, and its larvae eat asparagus stems and new leaves, seriously affecting the normal growth of asparagus
In the prior art, chemical pesticides are mostly used to prevent and control it. Due to the long-term unreasonable use of chemical pesticides, the drug resistance of Spodoptera litura is significantly enhanced, and the difficulty of its control is also increasing; Soil and surrounding water sources are polluted, seriously affecting the environment, and indirectly causing a certain degree of harm to humans and animals

Method used

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  • Application and method for controlling asparagus prodenia litura by utilizing beauveria bassiana
  • Application and method for controlling asparagus prodenia litura by utilizing beauveria bassiana
  • Application and method for controlling asparagus prodenia litura by utilizing beauveria bassiana

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preparation example Construction

[0027] The preparation method of described active Beauveria bassiana product comprises the following steps:

[0028] Isolation and purification of Beauveria bassiana from Spodoptera litura parasitized by Beauveria bassiana;

[0029] The isolated and purified Beauveria bassiana is expanded and cultivated to produce an active Beauveria bassiana product.

[0030] The present invention preferably selects Spodoptera litura parasitized by Beauveria bassiana from the asparagus field, and then isolates and purifies Beauveria bassiana from the selected Beauveria bassiana, the specific process of selecting Spodoptera litura parasitized by Beauveria bassiana from the asparagus field Preferably the following steps are included:

[0031] Clean the body of Spodoptera litura for 2 to 4 times with sterile water, and then disinfect the surface of the body with alcohol, the concentration of which is preferably 75%. After the disinfection is completed, the present invention transfers the steri...

Embodiment 1

[0049] Select the Spodoptera litura parasitized by Beauveria bassiana from the asparagus field, wash the selected Spodoptera litura three times with sterile water, and then disinfect the surface of the Spodoptera litura with 75% alcohol. Wash again in bacterial water for 2 minutes. After cleaning, put the worm body into an empty petri dish, cut the worm body into 5 parts, then inoculate it into solid nutrient agar medium, and cultivate it at 28°C for 15 hours; pick fresh mycelium and transfer it to a new The transfer culture was carried out in solid nutrient agar medium, each culture was 15 hours, and the transfer was performed 4 times to obtain isolated and purified Beauveria bassiana.

[0050] Inoculate the isolated and purified Beauveria bassiana in a liquid medium for liquid fermentation, inoculate the Beauveria bassiana in a liquid medium sterilized at 121°C for 35 minutes, and carry out liquid fermentation in a fermenter. The specific fermentation conditions: the tempera...

Embodiment 2

[0057] Select the Beauveria bassiana parasitized by Beauveria bassiana from the asparagus field, then isolate and purify Beauveria bassiana from the selected Beauveria bassiana, wash the body of Spodoptera litura twice with sterile water, and then use 75% alcohol to treat the surface of the worm body Disinfect, and after the disinfection is completed, transfer the worms to sterile water for another 3 minutes of cleaning. After cleaning, put the worm body into an empty petri dish, cut the worm body into 4 parts, and inoculate it into solid nutrient agar medium for 12 hours at 27°C; pick fresh hyphae for transfer culture, and fresh bacteria The silk was transferred to a new solid nutrient agar medium, cultivated for 12 hours, and transferred 5 times to obtain isolated and purified Beauveria bassiana.

[0058] Inoculate the isolated and purified Beauveria bassiana in a liquid medium for liquid fermentation, inoculate the Beauveria bassiana in a liquid medium sterilized at 121°C f...

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Abstract

The invention provides application for controlling asparagus prodenia litura by utilizing beauveria bassiana. The application specifically comprises the step of applying an active beauveria bassiana product into an asparagus field; a preparation method of the active beauveria bassiana comprises the following steps of separating and purifying the beauveria bassian from prodenia litura in which the beauveria bassiana parasitizes; performing expanding culture on the separated and purified beauveria bassiana, and preparing into an active beauveria bassiana product. According to the application of the beauveria bassiana in control of the asparagus prodenia litura, by utilizing the effects of high infection, parasitism, lethal and re-propagation of the beauveria bassiana to paragus prodenia litura, the paragus prodenia litura is prevented and controlled, and aims of reduction of the use of chemical pesticide, control of environmental pollution, realization of environment friendly production are achieved.

Description

technical field [0001] The invention relates to the field of biological control of insect pests, in particular to the application of Beauveria bassiana in the control of Spodoptera litura. Background technique [0002] Spodoptera litura belongs to Lepidoptera, Noctuidae, likes high temperature and drought, and has the characteristics of gluttony, clustering of young larvae and dispersal of older larvae. The feeding habits of Spodoptera litura are very miscellaneous, which can harm more than 220 kinds of plants in 99 families. In recent years, the insect has occurred frequently, seriously harming vegetables, peanuts, sweet potatoes, taro and other crops. According to the survey, Spodoptera litura occurs heavily in asparagus fields, and its larvae eat asparagus stems and new leaves, seriously affecting the normal growth of asparagus. In the prior art, chemical pesticides are mostly used to prevent and control it. Due to the long-term unreasonable use of chemical pesticides, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/04A01P7/04
CPCA01N63/30
Inventor 梁小文王根豪严艺波李肖宇曾升华石峥吴俊杰李英武
Owner JIANGXI TIANREN BIOLOGICAL HLDG CO LTD
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