Application and method for controlling asparagus prodenia litura by utilizing beauveria bassiana
A technology of Spodoptera litura and Beauveria bassiana, applied in the direction of botany equipment and methods, applications, pesticides, etc., can solve the problems of human and animal hazards, increased difficulty of prevention and control, and impact on the environment, so as to achieve reduced use and long-lasting effect Sexual growth, the effect of preventing and controlling environmental pollution
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[0027] The preparation method of described active Beauveria bassiana product comprises the following steps:
[0028] Isolation and purification of Beauveria bassiana from Spodoptera litura parasitized by Beauveria bassiana;
[0029] The isolated and purified Beauveria bassiana is expanded and cultivated to produce an active Beauveria bassiana product.
[0030] The present invention preferably selects Spodoptera litura parasitized by Beauveria bassiana from the asparagus field, and then isolates and purifies Beauveria bassiana from the selected Beauveria bassiana, the specific process of selecting Spodoptera litura parasitized by Beauveria bassiana from the asparagus field Preferably the following steps are included:
[0031] Clean the body of Spodoptera litura for 2 to 4 times with sterile water, and then disinfect the surface of the body with alcohol, the concentration of which is preferably 75%. After the disinfection is completed, the present invention transfers the steri...
Embodiment 1
[0049] Select the Spodoptera litura parasitized by Beauveria bassiana from the asparagus field, wash the selected Spodoptera litura three times with sterile water, and then disinfect the surface of the Spodoptera litura with 75% alcohol. Wash again in bacterial water for 2 minutes. After cleaning, put the worm body into an empty petri dish, cut the worm body into 5 parts, then inoculate it into solid nutrient agar medium, and cultivate it at 28°C for 15 hours; pick fresh mycelium and transfer it to a new The transfer culture was carried out in solid nutrient agar medium, each culture was 15 hours, and the transfer was performed 4 times to obtain isolated and purified Beauveria bassiana.
[0050] Inoculate the isolated and purified Beauveria bassiana in a liquid medium for liquid fermentation, inoculate the Beauveria bassiana in a liquid medium sterilized at 121°C for 35 minutes, and carry out liquid fermentation in a fermenter. The specific fermentation conditions: the tempera...
Embodiment 2
[0057] Select the Beauveria bassiana parasitized by Beauveria bassiana from the asparagus field, then isolate and purify Beauveria bassiana from the selected Beauveria bassiana, wash the body of Spodoptera litura twice with sterile water, and then use 75% alcohol to treat the surface of the worm body Disinfect, and after the disinfection is completed, transfer the worms to sterile water for another 3 minutes of cleaning. After cleaning, put the worm body into an empty petri dish, cut the worm body into 4 parts, and inoculate it into solid nutrient agar medium for 12 hours at 27°C; pick fresh hyphae for transfer culture, and fresh bacteria The silk was transferred to a new solid nutrient agar medium, cultivated for 12 hours, and transferred 5 times to obtain isolated and purified Beauveria bassiana.
[0058] Inoculate the isolated and purified Beauveria bassiana in a liquid medium for liquid fermentation, inoculate the Beauveria bassiana in a liquid medium sterilized at 121°C f...
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