Beauveria brongniartii BRNS50206 and application thereof

A technology of Beauveria bassiana and biological fungicides, applied in the field of agricultural biology, can solve problems such as environmental pollution and ecological damage, and achieve good control effects

Active Publication Date: 2019-02-15
INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the ecological damage and environmental pollution caused by chemical pesticides in the prior art to prevent and control pests, the present invention provides a highly efficient insecticidal Beauveria bassiana, which has a special control effect on specific diseases and insect pests, The characteristics of high biological safety, no pollution, no residue, and environmental friendliness

Method used

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  • Beauveria brongniartii BRNS50206 and application thereof
  • Beauveria brongniartii BRNS50206 and application thereof
  • Beauveria brongniartii BRNS50206 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1 Obtaining Beauveria bassiana

[0032] 1. Obtain high-efficiency strains

[0033] The present invention collects the dead insects infected with Beauveria from Shandong, Jiangsu, Hebei, Yunnan, Sichuan, Beijing, Inner Mongolia, etc., and separates and purifies the microorganisms in the laboratory.

[0034] Carefully pick the bacteria from the dead insects, inoculate them on the selective medium, and cultivate them at 25°C for 7 days, distinguish the colonies and transfer them to a new plate of selective medium, and cultivate them at 25°C for more than 14 days , after the colony is completely sporulated, it is transferred to a strain medium test tube, cultivated at a temperature of 25°C for 14 days, and stored in a refrigerator at 4°C.

[0035] The purified 63 bacterial strains were used for bioassay on the larvae of the black beetle. The specific method steps were as follows: the spores of each bacterial strain were mixed evenly with the soil, so that each gram...

Embodiment 2

[0042] The culture method of embodiment 2 bacterial strains

[0043] The culture method of bacterial strain of the present invention comprises the following steps:

[0044] (1) Liquid seed culture: the medium contains 3% sucrose, 1% peptone, 0.25% yeast extract powder, 0.01% magnesium sulfate, and the balance is water. Inoculate the spores of the test tube bacteria into the shake flask with the culture medium, the inoculation amount is 5×10 4 Spores / mL, shaking culture on a shaker at 25±1°C, 200rpm for 48h;

[0045] (2) Liquid secondary culture: the medium contains 2% sucrose, 1% soluble starch, 1% brewer's yeast, 0.4-0.6% dry carrot powder, 0.1% dipotassium hydrogen phosphate, 0.05% magnesium sulfate, and the balance is water. The liquid seed is inoculated into the fermenter, the inoculum amount is 3-5% according to the volume ratio, the temperature is controlled at 25±1° C., the rotation speed is 50-200 rpm, the ventilation rate is 0.5-0.8, and the fermentation is cultivat...

Embodiment 3

[0047] Example 3 Verification of the insecticidal ability of bacterial strains

[0048] 1. Verify the insecticidal ability of the strain spore powder

[0049] (1) The ability to kill scarab larvae

[0050] Add the spore powder obtained from the fermentation culture into the soil quantitatively, mix evenly, and form 1×10 per gram of soil 7 , 5×10 7 , 10×10 7 Different concentrations of spores were mixed with bacterial soil, divided into small plastic feeding boxes, and 1 grub caught in the wild was placed in each box, and potato pieces were added as feed. 20 grubs were treated at each concentration, repeated 3 times, and no bacteria powder was added. The blank soil was used as the control, cultivated at 23-26°C for 20 days, checked every 3 days, and determined the effect of the strain according to the comparison of the final mortality of grubs. In the experiment, it was observed that the dead worms became stiff, and the dead worms were transferred to a clean petri dish to k...

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Abstract

The invention belongs to the technical field of agricultural biology, and particularly relates to Beauveria brongniartii BRNS50206 and an application thereof. The Beauveria brongniartii BRNS50206 is taken as a biological insecticide, has high toxicity on target pests, can realize good control effect and facilitates reduction of use of chemical pesticides in plant protection, and meanwhile, a strain is non-toxic and harmless to people and livestock, is environmentally friendly and pollution-free and guarantees crop safety and ecological safety.

Description

technical field [0001] The invention belongs to the field of agricultural biotechnology, and in particular relates to Beauveria bassiana BRNS50206 and its application. Background technique [0002] Scarab and weevil pests cause serious harm to agriculture and forestry. For a long time, my country has mainly relied on chemical pesticides to control pests. Due to the large-scale use of chemical pesticides in successive years and the lack of efficient control methods, the resistance of pests has increased. The environment has been severely damaged, but pests are still rampant. At the same time, agricultural products, soil and waters are all polluted by pesticide residues, and human health is also seriously threatened and damaged. [0003] With the continuous improvement of people's living standards, people are paying more and more attention to food safety and environmental pollution, and their requirements for food quality are getting higher and higher. In order to protect th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N3/00A01N63/04A01P7/04C12R1/645
CPCA01N63/30C12N1/14C12N3/00C12N1/145C12R2001/645
Inventor 农向群王广君蔡霓张泽华
Owner INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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