A fungus sj18 of the family Cyclocerataceae and its application in the control of pine wood nematode

A technology of sj18 and pine wood nematode, applied in application, nematicides, chemicals for biological control, etc., can solve problems such as inability to control pine wood nematode disease, little progress, and unanswerable

Active Publication Date: 2021-08-27
ZHEJIANG FORESTRY ACAD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The problem with attractant control is that it is impossible to answer how many beetles will remain in the forest after the control, so obviously it cannot be used as the main method for the prevention and control of pine wood nematode disease
However, an infesting fungus that is sufficiently effective in deworming and fumigation has not yet been discovered, and little progress has been made in this area over the years, nor has it attracted enough attention.

Method used

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  • A fungus sj18 of the family Cyclocerataceae and its application in the control of pine wood nematode
  • A fungus sj18 of the family Cyclocerataceae and its application in the control of pine wood nematode
  • A fungus sj18 of the family Cyclocerataceae and its application in the control of pine wood nematode

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Embodiment 1: the isolation of Xylariaceae fungus

[0019] Take the root of a wild hickory tree, wash it, cut it into small pieces of about 2cm, first sterilize the surface with 70% alcohol, then soak it in 50% antifomin (sodium hypochlorite solution) for half an hour, and finally soak it in sterile water Rinse 4-5 times;

[0020] Cut the sterilized material on the ultra-clean bench, inoculate it on the PDA medium (solid potato medium) plate and cultivate it. When bacteria grow, they are respectively streaked and purified, and the DNA is extracted for molecular identification, so as to obtain And confirm the strain. The specific steps to confirm the strain are as follows:

[0021] 1) Pick the bacteria to be tested from the PDA plate and add them to the suspension solution A, add a sterilized tungsten carbide bead with a diameter of 3-5mm to the bacteria solution, put it in a boiling water bath for 5 minutes, and then place it in liquid nitrogen Under quick freezing, ...

Embodiment 2

[0037] Example 2: Morphological and biological characteristics of bacterial strains

[0038] Cyclobacteriaceae fungi ( Metarhizium anisopliae ) sj18 grows well on PDA medium, and the optimum temperature is 32-34°C. At 32°C, a 0.5cm block can grow to 8cm in 4 days. It can grow normally at pH5-11, and the growth rate is not affected. The mycelium is white when it first grows, and after 1 week of cultivation, the fungus turns dark brown and does not produce spores. figure 1 It is the morphology of sj18 bacteria under the microscope.

Embodiment 3

[0039] Embodiment 3: the effect of bacterial strain fermented liquid killing pine xylophilus

[0040] The specific implementation steps are as follows:

[0041] 1. Pine xylophilus collection

[0042] 1) Take a spare PDA plate, inoculate it with Botrytis cinerea, incubate at 25°C until the entire plate is covered, then store it upside down at 4°C for later use;

[0043] 2) Wrap the pine wood block with gauze, put it in a sealed plastic bag, add sterile water, soak in a water bath at 20-25°C overnight, centrifuge the soaking solution at 2000rpm for 3-5min, remove the supernatant, and leave 1-5mL, that is, for insect fluid;

[0044] 3) Take 20-50 μL of insect liquid and add it to the Botrytis cinerea plate covered with mycelia in step 1) in the form of a streak, grow at 25°C until the area of ​​the insect reaches more than 2 / 3 of the petri dish, and store in a 4-degree refrigerator for later use;

[0045] 2. nematode screening

[0046] 1) Take the nematode plate out of the ...

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Abstract

The invention relates to a fungus sj18 of the family Cyclocerataceae and its application in the control of pine wood nematode, belonging to the field of biotechnology. The present invention provides the function of the fungus sj18 and its secondary metabolites in the control of pine wood nematode. The fungus sj18 and its secondary metabolites of the present invention are derived from nature, are environmentally friendly and compatible, have no pollution and no residue, have high-efficiency inhibitory effect on pine wood nematodes, and can be used as new pollution-free biological pesticides for Harmless control of pine wilt disease caused by pine xylophilus infection.

Description

technical field [0001] The invention belongs to the field of biological technology, and in particular relates to the application of the fungus sj18 of the family Xylceraceae to the control of pine wood nematode. Background technique [0002] Pine wood nematode disease is a major international quarantine disease caused by pine tree wilting and death caused by parasites infected with pine trees - pine wood nematode. In 1982, my country first discovered pine wood nematode disease on black pine in Zijin Mountain, Nanjing City, and 265 dead trees. By 2017, pine wood nematode disease had occurred in more than 300 cities and counties in 18 provinces within 35 years. In my country, pine wood nematode disease is a typical pathogen-dominated disease, that is, the occurrence of the disease depends on whether there is a pathogen infection. Once infected, the result is death. Therefore, it is called "cancer" of pine trees. my country's national pine forest area has more than 500 million...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N63/30A01P5/00
CPCA01N63/30
Inventor 彭华正朱汤军金群英张飞英叶华琳
Owner ZHEJIANG FORESTRY ACAD
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