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Dioscorea zingiberensis endogenesis fusarium capable of producing beauvericin and antibacterial activity thereof

A technology of Beauveria and Dioscorea scutellaria, applied in the field of microorganisms, can solve the problems of separation and purification of single compounds that have not yet been seen

Inactive Publication Date: 2008-08-13
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] So far, there is no report on the isolation and purification of a single compound from the endophytic fungus of Dioscorea scutellaria

Method used

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  • Dioscorea zingiberensis endogenesis fusarium capable of producing beauvericin and antibacterial activity thereof
  • Dioscorea zingiberensis endogenesis fusarium capable of producing beauvericin and antibacterial activity thereof
  • Dioscorea zingiberensis endogenesis fusarium capable of producing beauvericin and antibacterial activity thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1: Isolation of endophytic fungus Dzf2

[0038] Collect fresh rhizomes of Dioscorea scutellaria, treat the surface with 70% ethanol for 30 seconds, then treat with 0.2% mercuric chloride for 20 minutes to carry out thorough surface sterilization, remove the epidermis under aseptic conditions, and divide the rhizomes into about 0.5 cm×0.5cm×0.5cm in size, placed on PDA medium, 1 piece per plate, cultured at 25°C under light conditions until the 7th to 20th day, pick a small piece from the edge of each colony The hyphae were inoculated on PDA medium for purification, and the purification was continued for 4-5 times until the colonies had the same morphology. The purified strains were preserved on the PDA slant, and one of the endophytic fungi was numbered Dzf2.

Embodiment 2

[0039] Embodiment 2: the antibacterial activity of endophyte Dzf2 bacterial strain extract

[0040] The endophytic fungus Dzf2 bacterial liquid and mycelia extract were detected by agar punching agent diffusion method, and the results are shown in Table 1.

[0041] Table 1 Antibacterial activity of endophytic fungus Dzf2 extract

[0042]

[0043] Note: The data in the table is the diameter of the inhibition zone ± standard error (cm); the amount of endophytic fungal extract in each well is 2.5 mg; streptomycin sulfate (positive control) is 10 μg per well; negative control is 50 μl formazan sulfoxide, showed no inhibitory activity.

[0044] The minimum inhibitory concentration (MIC) of endophytic fungus Dzf2 bacterial liquid and mycelia extract on bacterial growth was determined by multi-well plate liquid dilution method with thiazolium blue (MTT) as chromogen, and the results are shown in Table 2.

[0045] Table 2 The minimum inhibitory concentration of endophytic ...

Embodiment 3

[0048] Embodiment 3: Observation of the morphological characteristics of the endophytic fungus Dzf2 strain

[0049] Endophytic fungus Dzf2 is cultured on PDA medium at 25°C, producing a large number of aerial hyphae, white, with sparse hyphae at the edges, orange-yellow on the back of the colony, solitary or whorled conidiophores, phialide-shaped, and separated at the base , megaspores are nearly sickle-shaped, with 2-3 septa, microspores are oval, oval, reniform with no septum or 1 septum, spore size 4.3-24μm×2.4-5μm. The morphological feature maps are shown in Figures 1 to 4, respectively. Under culture conditions, no sexual generation of Dzf2 was found.

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Abstract

The invention relates to a dong-shaped Chinese potato endogenetic fusarin producing beauveria. The endogenetic fungal Dzf2 incorporating the invention is obtained from dong-shaped Chinese potato by endogenetic fungal isolation technology, and is classified and determined as fusarium by tiny organism. The bacterial strain has been preserved in china tiny organism culture preservation management committee commonness tiny organism local and accession identification serial number is CGMCC No.2340. The endogenetic fungal producing beauveria is first separated from dong-shaped Chinese potato and the antibacterial activity of beauveria is definituded. The object of the invention is to obtain savageness activity ingredient via plants endogenetic fungal resource.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a kind of microorganism whose metabolite is beauvericin-endophytic Fusarium scutellariae. Background technique [0002] Plant endophytic fungi refer to those fungi that live in healthy plant tissues or organs during a certain stage or all stages of their life history, and the host plant does not show external disease symptoms. Plant endophytic fungi are a new microbial resource that can produce metabolites of various structural types such as alkaloids, peptides, steroids, terpenes, phenols, quinones, aliphatics, isocoumarins, etc. , has various biological activities such as antibacterial, antiviral, antitumor, anti-insect, and regulation of plant growth. Because endophytic fungi can produce metabolites with novel structures and unique functions, they become potential resources for new compounds and new drugs, and they have important application prospects in the fields of ...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12P17/14C12R1/645
Inventor 周立刚姜微波彭友良王明安徐利剑黄永富赵江林
Owner CHINA AGRI UNIV
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