Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Beauveria bassiana strain and application thereof

A technology of Beauveria bassiana and strains, applied in the application, fungi, biocide and other directions, can solve the problems of poor insect control effect, poor effect, environmental pollution, etc., and is conducive to large-scale production and promotion Application, good pathogenic effect, good control effect

Inactive Publication Date: 2019-07-12
SOUTHWEST FORESTRY UNIVERSITY
View PDF6 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to the biological characteristics exhibited by bark beetles, it can be seen that its harm is hidden. For the bark beetles that have been bored in host tree species for a long time, the use of chemical agents is often not effective, and chemical control has a major impact on environmental pollution. disadvantages
The radiation and fumigation in the physical control methods are aimed at the control of quarantine bark beetles, and are not suitable for the control of living host plants. The physical method of pruning diseased branches is not very effective in controlling insects.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Beauveria bassiana strain and application thereof
  • Beauveria bassiana strain and application thereof
  • Beauveria bassiana strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Beauveria bassiana Beauveria bassiana Acquisition, identification and preservation of fungal strain BBXDCSFJ03.

[0054] (1) Beauveria bassiana Beauveria bassiana The acquisition and identification of fungal strain BBXDCSFJ03.

[0055] Beauveria bassiana of the present invention Beauveria bassiana The fungal strain BBXDCSFJ03 was obtained from the infected insects of the triangular maple beetle. The infected insects of the triangular maple beetle were collected from the triangular maple plantations in Cuihu Park, Heilongtan Park, Botanical Garden and other major parks in Kunming. The branches of the triangular maple with back withered symptoms were taken back to the laboratory for dissection, the healthy ones were reared indoors, and the dead ones were isolated and cultured for pathogenic bacteria.

[0056] Wash the red imported fire ants with a 75% ethanol solution for 5 seconds, then disinfect the surface of the ants with a 0.1% mercury solution for 2-3 minutes,...

Embodiment 2

[0068] Beauveria bassiana Beauveria bassiana Preparation of biocontrol agent by fungal strain BBXDCSFJ03

[0069] (1) Preparation of spore suspension.

[0070] First, Beauveria bassiana Beauveria bassiana The fungal strain BBXDCSFJ03 was inoculated on ordinary PDA medium and cultured at a constant temperature of 25°C ± incubator for 7-15d.

[0071] The components of the PDA solid medium are preferably in g / L: 200 g of potatoes, 20 g of glucose, 20 g of agar, and a natural pH.

[0072] Sterilized sterile water was then added with 0.05% Tween-80.

[0073] Rinse the spores from the Petri dish with sterile aqueous solution.

[0074] Pour the bacteria solution and spores in the petri dish into the cup and shake it for 30 minutes, filter it with a lens tissue, and finally get the uncounted spore suspension.

[0075] Take out the hemocytometer, place it under the microscope, and record the number of all spores in the inner 400 cells.

[0076] Finally calculate the total spore...

Embodiment 3

[0080] Beauveria bassiana Beauveria bassiana The fungal strain BBXDCSFJ03 was used to prepare biocontrol agent.

[0081] (1) Preparation of spore suspension.

[0082] First, Beauveria bassiana Beauveria bassiana The fungal strain BBXDCSFJ03 was inoculated on ordinary PDA medium and cultured in a 26°C incubator for 7-15 days.

[0083] The components of the PDA solid medium are preferably in g / L: 200 g of potatoes, 20 g of glucose, 20 g of agar, and a natural pH.

[0084] Sterilized sterile water was then added with 0.05% Tween-80.

[0085] Rinse the spores from the Petri dish with sterile aqueous solution.

[0086]Pour the bacteria solution and spores in the petri dish into the cup and shake it for 30 minutes, filter it with a lens tissue, and finally get the uncounted spore suspension. Take out the hemocytometer, place it under the microscope, and record the number of all spores in the inner 400 cells.

[0087] Finally calculate the total spore number of the spore susp...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a Beauveria bassiana strain and application thereof. The Beauveria bassiana strain belongs to the class of Beauveria bassiana, the preservation unit is the China General Microbiological Culture Collection Center (CGMCC), the preservation date is December 28, 2018, the preservation number is CGMCC No.17068, and Beauveria bassiana belongs to fungus, deuteromycota, hyphomycetes, moniliales, moniliaceae, beauveria. According to the application, the Beauveria bassiana strain is applied to the preparation of a biological preparation for preventing and controlling euwallacea fornicatus.

Description

technical field [0001] The invention belongs to the technical field of microorganism isolation and cultivation, and in particular relates to a strain of Beauveria bassiana and application thereof. Background technique [0002] Triangle maple, an important garden tree species in Kunming, was affected by the bark beetle (Little beetle Euwallacea fornicatus ), severely damaged the landscape construction of Kunming City and caused huge economic losses, and it is urgent to control bark beetles. Because the bark beetle is small and difficult to find, and its life is concealed, the host provides a good barrier for it to complete its life cycle, making it difficult to control; and the small round-breasted beetle is more difficult to control because of its numerous host plants and wide distribution (Walgama R S. et al., 2012). Bark beetles are only active in the outside world during a period of time when they are new adults, and spend most of their life in hiding, usually mating an...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/04A01P7/04C12R1/645
CPCC12R2001/645C12N1/145A01N63/30
Inventor 马焕成伍建榕魏玉倩洪永生赵长林马翔龙娟武自强
Owner SOUTHWEST FORESTRY UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com