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Purpureocillium lilacinum strain and application thereof

A technology of bacterial strains and fungi, applied in the direction of application, fungi, biocides, etc., to achieve simple operation procedures, conducive to large-scale production and popularization of applications, and good control effects

Active Publication Date: 2019-08-16
SOUTHWEST FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The area that will be invaded by red fire ants will increase rapidly in the future, which will inevitably lead to an increase in the use of chemical agents, but traditional chemical control will lead to serious 3R problems

Method used

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  • Purpureocillium lilacinum strain and application thereof
  • Purpureocillium lilacinum strain and application thereof
  • Purpureocillium lilacinum strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] P. lilacinus (synonym: Paecilomyces spp.) fungal strain ( Purpureocillium lilacinum ) Acquisition, identification and preservation of PLHHYSFJ02.

[0060] (1) P. lilacinus (synonym: Paecilomyces) fungal strain ( Purpureocillium lilacinum ) Acquisition and identification of PLHHYSFJ02.

[0061] The fungus of P. lilacinus (synonym: Paecilomyces genus) described in the present invention is isolated from RIFA, and RIFA is collected from East Third Ring Road, Panlong District, Chenggong District, East Sichuan District, Mangshi, Dehong Dai and Jingpo Autonomous Prefecture. The complete nests with RIFA queens were reared in the laboratory.

[0062] Wash the red imported fire ants with a 75% ethanol solution for 5 seconds, then disinfect the surface of the ants with a 0.1% mercury solution for 2-3 minutes, slowly rinse with sterile water for 3 times, and use sterilized The tweezers separate the body of the RIFA according to different parts of the head, thorax and abdomen,...

Embodiment 2

[0074] A strain of P. lilacinus (synonym: Paecilomyces spp.) fungal strain ( Purpureocillium lilacinum ) PLHHYSFJ02 to prepare biocontrol agents.

[0075] (1) Preparation of spore suspension.

[0076] First, the P. lilacinus (synonym: Paecilomyces sp.) fungal strain ( Purpureocillium lilacinum ) The PLHHYSFJ02 strain was inoculated on ordinary PDA medium and cultured at a constant temperature of 25 °C ± incubator for 15 days.

[0077] The components of the PDA solid medium are preferably in g / L: 200 g of potatoes, 20 g of glucose, 20 g of agar, and a natural pH.

[0078] Sterilized sterile water was then added with 0.05% Tween-80.

[0079] Rinse the spores from the Petri dish with sterile aqueous solution.

[0080] Pour the bacteria solution and spores in the petri dish into the cup and shake it for 30 minutes, filter it with a lens tissue, and finally get the uncounted spore suspension.

[0081] Take out the hemocytometer, place it under the microscope, and record th...

Embodiment 3

[0086] P. lilacinus (synonym: Paecilomyces spp.) fungal strain ( Purpureocillium lilacinum ) PLHHYSFJ02 to prepare biocontrol agents.

[0087] (1) Preparation of spore suspension.

[0088] First, the P. lilacinus (synonym: Paecilomyces sp.) fungal strain ( Purpureocillium lilacinum ) PLHHYSFJ02 strain was inoculated on ordinary PDA medium and cultured in a 26°C incubator for 15 days.

[0089] The components of the PDA solid medium are preferably in g / L: 200 g of potatoes, 20 g of glucose, 20 g of agar, and a natural pH.

[0090] Sterilized sterile water was then added with 0.05% Tween-80.

[0091] Rinse the spores from the Petri dish with sterile aqueous solution.

[0092] Pour the bacteria solution and spores in the petri dish into the cup and shake it for 30 minutes, filter it with a lens tissue, and finally get the uncounted spore suspension.

[0093] Take out the hemocytometer, place it under the microscope, and record the number of all spores in the inner 400 cells...

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Abstract

The invention discloses a Purpureocillium lilacinum strain and application thereof. The Purpureocillium lilacinum strain is classified and named as Purpureocilium lilacinum, and was deposited at deposited at China General Microbiological Culture Collection Center (CGMCC) with accession number of CGMCC No. 17067 on December 28, 2018. The Purpureocillium lilacinum belongs to kingdom fungi, eumycota,fungi imperfecti, cnidosporales, Hyphomycetes, family Hyphomycetes and Purpureocillium lilacinum. The Purpureocillium lilacinum strain is applied to preparation of biological preparation for biological control of fire ants.

Description

technical field [0001] The invention belongs to the technical field of microorganism isolation and cultivation, and in particular relates to a fungus strain of the genus Viola lilacinus and its application. Background technique [0002] On September 23, 2004, the Plant Quarantine Station of Wuchuan City reported the serious damage of the local red imported fire ants. On January 17, 2005, my country added RIFA as an inspection pest of imported plants, a national plant quarantine pest, a national forestry quarantine pest, and a national forestry dangerous pest. [0003] The rapid invasion of red imported fire ants is due to the strong fecundity and attack ability, and the rapid population growth leads to high density of ant nests. The red imported imported imported imported imported imported imported imported imported imported imported imported imported imported imported fire ants originated from South America. The adults have a variety of food habits. The red imported import...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/04A01P7/04C12R1/645
CPCC12N1/14C12R2001/645C12N1/145A01N63/30Y02A50/30
Inventor 洪永生伍建榕马焕成魏玉倩洪英娣马翔张东华闫晓慧
Owner SOUTHWEST FORESTRY UNIVERSITY
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