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Paecilomyces lilacinus fungus strain and application thereof

A technology of Paecilomyces lilacinus and bacterial strains, applied in the fields of application, fungi, and methods of using spores, etc., can solve problems such as poor insect control effect, poor effect, and environmental pollution, and is conducive to large-scale production and popularization and application, good pathogenic effect and good control effect

Inactive Publication Date: 2019-07-09
SOUTHWEST FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to the biological characteristics exhibited by bark beetles, it can be seen that its harm is hidden. For the bark beetles that have been bored in host tree species for a long time, the use of chemical agents is often not effective, and chemical control has a major impact on environmental pollution. disadvantages
The radiation and fumigation in the physical control methods are aimed at the control of quarantine bark beetles, and are not suitable for the control of living host plants. The physical method of pruning diseased branches is not very effective in controlling insects.

Method used

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  • Paecilomyces lilacinus fungus strain and application thereof
  • Paecilomyces lilacinus fungus strain and application thereof
  • Paecilomyces lilacinus fungus strain and application thereof

Examples

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Effect test

Embodiment 1

[0057] Paecilomyces lilacinus ( Paecilomyces lilacinus ) Acquisition, identification and preservation of the fungal strain PLXDCSFJ04.

[0058] (1) Paecilomyces lilacinus ( Paecilomyces lilacinus ) Acquisition and identification of fungal strain PLXDCSFJ04.

[0059] Paecilomyces lilacinus described in the present invention ( Paecilomyces lilacinus ) The fungal strain PLXDCSFJ04 fungus was obtained from the infected insects of the triangular maple beetle. The branches of the triangular maple with symptoms of withering were taken back to the laboratory for dissection, the healthy ones were kept indoors, and the dead ones were isolated and cultured for pathogenic bacteria.

[0060] Wash the red imported fire ants with a 75% ethanol solution for 5 seconds, then disinfect the surface of the ants with a 0.1% mercury solution for 2-3 minutes, slowly rinse with sterile water for 3 times, and use sterilized The tweezers separated the body of the triangular maple beetle according to...

Embodiment 2

[0072] Paecilomyces lilacinus fungal strain ( Paecilomyces lilacinus ) Preparation of biocontrol agent by fungal strain PLXDCSFJ04

[0073] (1) Preparation of spore suspension.

[0074] First, the Paecilomyces lilacinus fungus strain ( Paecilomyces lilacinus ) The fungal strain PLXDCSFJ04 was inoculated on ordinary PDA medium and cultured at a constant temperature of 25°C ± incubator for 7-15d.

[0075] The components of the PDA solid medium are preferably in g / L: 200 g of potatoes, 20 g of glucose, 20 g of agar, and a natural pH.

[0076] Sterilized sterile water was then added with 0.05% Tween-80.

[0077] Rinse the spores from the Petri dish with sterile aqueous solution.

[0078] Pour the bacteria solution and spores in the petri dish into the cup and shake it for 30 minutes, filter it with a lens tissue, and finally get the uncounted spore suspension.

[0079] Take out the hemocytometer, place it under the microscope, and record the number of all spores in the inner ...

Embodiment 3

[0084] Paecilomyces lilacinus fungal strain ( Paecilomyces lilacinus ) fungal strain PLXDCSFJ04 to prepare biocontrol agents.

[0085] (1) Preparation of spore suspension.

[0086] First, the Paecilomyces lilacinus fungus strain ( Paecilomyces lilacinus ) The fungal strain PLXDCSFJ04 was inoculated on ordinary PDA medium and cultured in a 26°C incubator for 7-15 days.

[0087] The components of the PDA solid medium are preferably in g / L: 200 g of potatoes, 20 g of glucose, 20 g of agar, and a natural pH.

[0088] Sterilized sterile water was then added with 0.05% Tween-80.

[0089] Rinse the spores from the Petri dish with sterile aqueous solution.

[0090] Pour the bacteria solution and spores in the petri dish into the cup and shake it for 30 minutes, filter it with a lens tissue, and finally get the uncounted spore suspension. Take out the hemocytometer, place it under the microscope, and record the number of all spores in the inner 400 cells.

[0091] Finally calculate...

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Abstract

The invention discloses a paecilomyces lilacinus fungus strain and application thereof. The paecilomyces lilacinus fungus strain is classified and named Paecilomyces lilacinus, the depositary authority is China General Microbiological Culture Collection Center (CGMCC), the preservation date is December 28, 2018, the preservation number is CGMCC No.17069, a paecilomyces lilacinus fungus belongs tokingdom fungi, eumycota, fungi imperfecti, hyphomycetales, moniliaceae and paecilomyces. The application is the application of the paecilomyces lilacinus fungus strain in preparing a biological preparation for preventing and controlling acer buergerianum xyleborus.

Description

technical field [0001] The invention belongs to the technical field of microorganism isolation and cultivation, and in particular relates to a Paecilomyces lilacinus fungus strain and an application thereof. Background technique [0002] The triangular maple, an important garden tree species in Kunming, is harmed by the bark beetle, which has seriously damaged the landscape construction of Kunming and caused huge economic losses. The control of the bark beetle is urgent. Because the bark beetle is small and difficult to find, and its life is concealed, the host provides a good barrier for it to complete its life cycle, making it difficult to control; and the small round-breasted beetle is more difficult to control because of its numerous host plants and wide distribution (Walgama R S. et al., 2012). Bark beetles are only active in the outside world during a period of time when they are new adults, and spend most of their life in hiding, usually mating and feeding under th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N3/00A01N63/04A01P7/04C12R1/80
CPCC12N1/14C12N3/00C12R2001/80C12N1/145A01N63/30
Inventor 伍建榕马焕成魏玉倩洪英娣马翔刘丽洪永生龙娟
Owner SOUTHWEST FORESTRY UNIVERSITY
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