Application of GRMZM2G417164 in stomata development

A GRMZM2G41716, gene technology, applied in the field of corn genetic engineering, can solve problems such as research lag

Active Publication Date: 2018-02-23
HENAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few related stomatal mutants in maize, and the research lags behind

Method used

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  • Application of GRMZM2G417164 in stomata development
  • Application of GRMZM2G417164 in stomata development
  • Application of GRMZM2G417164 in stomata development

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specific Embodiment

[0031] The present invention will be further explained in conjunction with the following examples. Before introducing specific examples, a brief introduction to the background of some biological materials involved in the following examples is as follows.

[0032] biomaterials:

[0033] corn GRMZM2G417164 mutant ( bzu2-1 mutant) and its wild-type counterpart, are publicly available material from the Maize Genetics Cooperation Stock Center, named bzu2-1 ; It needs to be explained that, before this application for bzu2-1 Mutant genes were not identified, i.e., bzu2-1It is unknown which gene is mutated in the mutant.

Embodiment

[0035] Since the relevant technical solutions of this application are highly related to the observation of related mutant phenotypes and the identification and confirmation of related genes, the corn GRMZM2G417164 Phenotypic Abnormal Discovery of Gene Mutants and Their Mutant Genes GRMZM2G417164 The gene identification process is briefly described below.

[0036] (1) Corn bzu2-1 Abnormal phenotypes of mutants

[0037] the wild-type (maize GRMZM2G417164 gene normal expression strain) and bzu2-1 The mutants were planted in the experimental field at the same time, with normal photoperiod and planting conditions. One week later, the seeds germinated and came out of the soil, and the wild-type seedlings grew normally, but bzu2-1 Seedlings appear hydrated, and further yellow and die at the three-leaf stage (eg figure 1 A).

[0038] For dead plants ( bzu2-1 mutant plants) leaves were microscopically observed, and it was found that the stomata were abnormally developed, a...

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Abstract

The invention relates to the technical field of maize gene engineering, in particular to application of a maize GRMZM2G417164 gene in adjustment of stomata development. The maize GRMZM2G417164, serving as a transcription factor, regulates some genes associated with the stomata development, and adjusts an associated process of maize stomata development. A series of experiments prove that a proteincoded by the GRMZM2G417164 gene controls transfer of a signal from a guard mother cell to a subsidiary mother cell and a final longitudinal division of the guard mother cell to form a guard cell. After the gene is mutant, a plant cannot form a normal guard cell and a normal subsidiary cell. Therefore, deep research and analysis on GRMZM2G417164 gene functions has an important theoretical significance and a practical application value for exploring a maize stomata development mechanism and improving maize resistance.

Description

technical field [0001] The invention relates to the technical field of corn genetic engineering, in particular to corn GRMZM2G417164 Patent applications for the use of genes in regulating stomatal development. Background technique [0002] Stomata are small pores on plant leaves that are opened or closed by two guard cells and are responsible for plant water loss and CO 2 The gateway to and from plants is critical for plant growth and development. Stomata mainly exist in terrestrial plants, and are also the key evolution of plants from aquatic to terrestrial. The presence of stomata enables plants to adapt to the terrestrial environment. [0003] In monocots, the stomata consist of a pair of guard cells surrounded by two accessory cells. The formation of accessory cells is induced by guard cell blasts and develops through asymmetric division of accessory cell blasts, mainly assisting guard cells to regulate the opening and closing of stomata. Stomatal development is infl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29A01H5/12A01H6/46
CPCC07K14/415C12N15/8271
Inventor 宋纯鹏王宏亮郭思义郭建飞李祖亮乔鑫王倩柏胜龙
Owner HENAN UNIVERSITY
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