Application of GRMZM2G417164 in stomata development
A GRMZM2G41716, gene technology, applied in the field of corn genetic engineering, can solve problems such as research lag
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[0031] The present invention will be further explained in conjunction with the following examples. Before introducing specific examples, a brief introduction to the background of some biological materials involved in the following examples is as follows.
[0032] biomaterials:
[0033] corn GRMZM2G417164 mutant ( bzu2-1 mutant) and its wild-type counterpart, are publicly available material from the Maize Genetics Cooperation Stock Center, named bzu2-1 ; It needs to be explained that, before this application for bzu2-1 Mutant genes were not identified, i.e., bzu2-1It is unknown which gene is mutated in the mutant.
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[0035] Since the relevant technical solutions of this application are highly related to the observation of related mutant phenotypes and the identification and confirmation of related genes, the corn GRMZM2G417164 Phenotypic Abnormal Discovery of Gene Mutants and Their Mutant Genes GRMZM2G417164 The gene identification process is briefly described below.
[0036] (1) Corn bzu2-1 Abnormal phenotypes of mutants
[0037] the wild-type (maize GRMZM2G417164 gene normal expression strain) and bzu2-1 The mutants were planted in the experimental field at the same time, with normal photoperiod and planting conditions. One week later, the seeds germinated and came out of the soil, and the wild-type seedlings grew normally, but bzu2-1 Seedlings appear hydrated, and further yellow and die at the three-leaf stage (eg figure 1 A).
[0038] For dead plants ( bzu2-1 mutant plants) leaves were microscopically observed, and it was found that the stomata were abnormally developed, a...
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