Continuous recessive character backcrossing technology
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A technology of recessive traits and technology, applied in the field of continuous backcross technology for recessive traits, can solve problems such as insufficient breeding technology routes
Inactive Publication Date: 2010-02-03
LIANYUNGANG ACAD OF AGRI SCI
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Problems solved by technology
At present, there have been reports on the successful breeding of two-line CMS lines with purple leaf markers in China. However, due to the lack of technical routes for the selection of purple leaf marker lines, the selected sterile lines carry a large number of unfavorable genes. The combination with the purple leaf marker male sterile line passed the examination and approval
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Embodiment 1
[0040] Example 1. refer to figure 1 . A continuous backcross technique for recessive traits, the steps of which are as follows:
[0041] (1) The material to be improved without the target recessive gene is used as the recurrent parent, and its genotype is AA, and the material containing the target recessive gene aa is used as the donor parent; the recurrent parent is used as the female parent to cross with the donor parent, Or prepare a backcross combination to harvest the first generation of hybrid F 1 with reincarnated parent seeds;
[0042] (2) Planting hybrid first generation F 1 Seeds with the reincarnated parent, with the reincarnated parent as the female parent and the first generation of hybrid F 1 Crossbreed to generate backcross generation BC 1 f 1 Seed, Harvest Backcross Generation BC 1 f 1 with reincarnated parent seeds;
[0043] (3) Planting backcross generation BC 1 f 1 Seeds with the reincarnated parent, with the reincarnated parent as the female par...
Embodiment 2
[0047] Example 2. In step (4) of embodiment 1, from BC 1 f 2 In the process of selecting recessive traits, single plants are crossed with recurrent parents, and BC is harvested in pairs at the same time 2 f 1With the reincarnation parent seed, as a backup branch.
Embodiment 3
[0048] Example 3. A kind of creation method of the purple leaf marker isogenic line of the continuous backcrossing technology of the recessive character described in application embodiment 1, its steps are as follows:
[0049] (1) The material to be improved without the purple leaf trait is used as the reincarnation parent, and the purple leaf material is used as the donor parent;
[0050] Use the recurrent parent as the female parent to cross with the donor parent, or prepare a backcross combination; harvest the first generation of hybrid F 1 with reincarnated parent seeds;
[0051] (2) Planting hybrid first generation F 1 With the seeds of the reincarnated parent, cross the first generation of hybrids with the reincarnated parent as the female parent to produce the first generation of backcrossed seeds; harvest the first generation of backcrossed BC 1 f 1 with reincarnated parent seeds;
[0052] (3) Planting backcross generation BC 1 f 1 With the seeds of the recurren...
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Abstract
The invention discloses a continuous recessive character backcrossing technology which is characterized by taking a material which contains no target recessive genes and is ready to be improved as a recurrent parent, and taking a material which contains the target recessive genes as a donor parent; taking the recurrent parent as the parent to be hybridized with the donor parent and harvesting hybrid seeds of the first generation F1 and recurrent parent seeds; and planting the hybrid seeds of the first generation F1 and the recurrent parent seeds till the planting of a group containing backcross hybrid seeds of the second of n generations BCn F2 and till the typing into a target recessive gene isogenic line of the recurrent parent. The invention also discloses a method for creating a purpleleaf marker isogenic line by applying the continuous recessive character backcrossing technology and also a method for transferring new purple leaf marker nucleus sterile line by taking the purple leaf marker isogenic line as an intermediate material. In the methods, a selfing process is combined with a next backcrossing process for the treatment in one season, therefore, the time efficiency is improved by about 50 percent, a small number of dominant-gene-controlled unsuitable characters of existing fine-quality parents can be fast improved, and useful recessive characters can be fast imported to the present high-level heredity background.
Description
technical field [0001] The invention relates to a hybrid rice combination breeding method, in particular to a continuous backcrossing technique for recessive traits. Background technique [0002] The backcrossing technology of plant recessive genes in the prior art is generally realized by the method of backcrossing at intervals, so the backcrossing process is slow, and the time efficiency is nearly 50% slower than the backcrossing speed of dominant genes. The situation has been very unfavorable. [0003] Recently, in China, due to the risk of fertility in the CMS itself in the two-line hybrid rice seed production, there has been an upsurge in research on marker traits CMS, such as albino markers, yellowing markers, light green leaf markers, and colored lemma husk markers. , Bentasone sensitive gene markers, draped leaf markers, purple leaf markers, etc. The genetic rules of various marker methods are also basically clear, but compared with the selection criteria such as ey...
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