Bicistronic mRNA coexpression gene transporter and preparation method thereof

A co-expression gene and gene transfer technology, applied in the biological field, can solve rare problems such as construction and application
CN102994536AInactive Publication Date: 2013-03-27INNER MONGOLIA UNIVERSITY
2 Cites 11 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
INNER MONGOLIA UNIVERSITY
Publication Date
2013-03-27
Estimated Expiration
Not applicable · inactive patent

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention relates to a bicistronic mRNA coexpression gene transporter and a preparation method thereof. The gene order of the gene transporter is SEQ ID No. 15; the gene transporter from 5' end to 3' end comprises bovine Nuclear matrix binding region MAR, artificially constructed combined promoter CAG, Profilin gene FSTN, internal ribosome entry site (IRES), green fluorescent protein AcGFP gene and Rabbit globin poly A signal region; the preparation method comprises the following steps: constructing a carrier pCAG-IRES2-AcGFP1; obtaining FSTN gene and inserting the pCAG-IRES2-AcGFP1 carrier; obtaining the sequence of MAR and inserting the pCAG-IRES2-AcGFP1 carrier; constructing a plasmid carriers so as to obtain the bicistronic mRNA coexpression gene transporter. The gene transporter is not only pure and safe gene transporter but also realizes dual-gene coexpression in any combination, achieves the purpose of multi-gene coexpression through repeated utilization of IRES, and provides new thinking and route for improving the shape of dual or multiple gene control such as economic character.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention belongs to the genetic engineering technology in the field of biotechnology, and in particular relates to a preparation method of a bicistronic co-expression gene transfer body. Background technique

[0002] High-efficiency expression of foreign genes in transgenic animals must rely on good expression vectors. There are many factors that affect the high-efficiency expression of foreign genes, such as promoters, methylation, gene structure, insertion sites, regulatory sequences (enhancers, insulators, nuclear matrix binding regions), etc.

[0003] The CAG promoter is an artificially constructed combined promoter consisting of the early enhancer element of the cytomegalovirus (CMV) and the chicken beta-actin promoter. The CAG promoter is Nonspecific constitutive promoters for driving high-level expression of genes in mammalian vectors. CMV is a commonly used promoter. In the application of our transgenic sheep, it was found that methylati...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More