Primers, probe, kit and method for accurate quantitative digital PCR detection of dog-derived component

A technology for quantitative detection and source components, applied in biochemical equipment and methods, microbiological determination/inspection, DNA/RNA fragments, etc., can solve the deviation of quantitative results of amplification efficiency, the accuracy error of template copy number, and improve detection Cost and other issues, to achieve stable and reliable results, reliable results, accurate and sensitive results
CN107488708AInactive Publication Date: 2017-12-19CHINESE ACAD OF INSPECTION & QUARANTINE
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
CHINESE ACAD OF INSPECTION & QUARANTINE
Publication Date
2017-12-19
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to oligonucleotide primers and a probe for accurate and quantitative detection of a dog-derived component. The invention also relates to a digital PCR detection method for quantitative detection of the dog-derived component. The method comprises a step of utilizing the specific oligonucleotide primers and probe directed at a dog-derived single-copy nuclear gene. The invention also relates to a digital PCR detection kit for quantitative detection of the dog-derived component. The kit comprises the specific oligonucleotide primers and probe for digital PCR detection of the dog-derived component. The invention further relates to application of the specific oligonucleotide primers and probe directed at the dog-derived component to quantitative detection of the dog-derived component. With the digital PCR detection method and kit of the invention, whether fresh meat and preliminarily processed meat products contain the dog-derived component or not can be specifically, sensitively and accurately detected.
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Description

technical field

[0001] The invention belongs to the field of biotechnology. Specifically, the invention relates to oligonucleotide primers and probes for the detection of dog-derived components, a digital PCR detection method for determining dog-derived components, and for accurate and quantitative detection of dog-derived components. Application of a digital PCR detection kit for dog-derived components and specific oligonucleotide primers and probes for dog-derived components in the detection of dog-derived components. Background technique

[0002] Meat and its products are rich in nutrients, with a protein content of about 10%-20%. Livestock meat is rich in B vitamins. Poultry meat has a high content of unsaturated fatty acids, such as linoleic acid, which is necessary for the human body, accounting for about 10% of the total fat content. 20%. The production and consumption of meat products across the country continue to increase. In 2013, my country became the largest me...

Claims

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