Creating method of chili S (RfRf) type cytoplasmic male sterility restoring lines

A technology of cytoplasmic male sterility and restorer lines, applied in botany equipment and methods, application, plant gene improvement, etc., can solve the problems of high cost of seed production, weak profit of pepper seeds, etc., and achieve the improvement of breeding efficiency and breeding goals The effects of controllable traits and procedures, and simple breeding procedures

Inactive Publication Date: 2017-01-04
ZHENJIANG AGRI SCI INST JIANGSU HILLY AREAS
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AI Technical Summary

Problems solved by technology

However, pepper hybrid seed production is a work that consumes a lot of labor, and the cost of seed production is huge. In

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The Method of Creating S(RfRf) Cytoplasmic Male Sterility Restorer Line SC02

[0027] Step 1. In the spring of 2011, the capsicum cytoplasmic male sterile line CMS-A2, whose genotype is S(rfrf) was used as the female parent, and its restorer line C02, whose genotype was N(RfRf) was used as the male parent, to obtain F 1 166 generation seeds.

[0028] Step 2, in the fall of 2011, the F 1 Sow 10 seeds on behalf of the seed, the F 1 Generation seeds, genotype S(Rfrf) are all fertile types, allowing them to naturally self-cross to obtain F 2 generation seeds.

[0029] Step 3, in the spring of 2012, the F 2 20 generations of seed sowing, its genotype is divided into three types, respectively S (RfRf), S (Rfrf) and S (rfrf), the fertility is respectively fertile, fertile and sterile, the fertile plant ( 14 plants) were numbered separately, and the pollen of the fertile plants was tested with the sterile plants (6 plants). Each fertile plant had at least 4 flowers for the...

Embodiment 2

[0035] The Method of Creating S(RfRf) Type Cytoplasmic Male Sterility Restorer Line SY26

[0036] Step 1. In the spring of 2012, the capsicum cytoplasmic male sterile line CMS-A2, whose genotype is S(rfrf), was used as the female parent, and its restorer line Y26, whose genotype was N(RfRf), was used as the male parent to obtain F 1 48 substitute seeds.

[0037] Step 2, in the fall of 2012, the F 1 10 generation seeds are sown, and the F1 generation seeds, whose genotype is S(Rfrf), are all fertile types, allowing them to naturally self-cross to obtain F 2 generation seeds.

[0038] Step 3, in the spring of 2013, the F 2 20 generations of seed sowing, its genotype is divided into three types, respectively S (RfRf), S (Rfrf) and S (rfrf), the fertility is respectively fertile, fertile and sterile, the fertile plant ( 15 plants) were numbered separately, and the pollen of the fertile plants was tested with the sterile plants (5 plants). Each fertile plant had at least 4 flow...

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PUM

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Abstract

The invention provides a creating method of chili S (RfRf) type cytoplasmic male sterility restoring lines. The method is characterized in that existing sterile line materials are used as the female parents, existing N (RfRf) materials are used as the male parents, the cytogenes of the female parents are taken and combined with the nuclear genes of the male parents to obtain target materials, the target-genotype intermediate materials are bred fast, and breeding reliability, accuracy and efficiency are increased. The creating method has the advantages that the method is simple in breeding procedure, objective traits and procedures of breeding can be controlled, breeding efficiency is increased greatly, the types and number of the bred restoring lines are increased greatly, and a new approach is provided for chili heterosis utilization and sterility line breeding and creating and the like.

Description

technical field [0001] The invention belongs to the technical field of plant breeding, and in particular relates to a method for creating a restorer line of capsicum S(RfRf) cytoplasmic male sterility. Background technique [0002] CMS refers to cytoplasmic male sterility (cytoplasmic male sterility) in crop breeding. Pepper cytoplasmic male sterile lines are controlled by cytoplasmic genes and nuclear genes respectively, and the cytoplasmic genes are respectively represented by N (fertile) and S ( sterility) and nuclear genes are denoted by Rf (fertile) and rf (sterile), respectively. Those with N-type cytoplasm are all fertile, and those with S-type cytoplasm are all fertile except for S(rfrf). According to previous experiments, 90% of pepper materials have genotypes of N(rfrf), which is a fertile species. The fertile material of quality control, and the capsicum material with recovery ability are basically N(RfRf), and it is basically inferred that the N-type cytoplasm...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01H1/04
CPCA01H1/02A01H1/04
Inventor 孙国胜马志虎孙春青戴忠良毛忠良潘跃平吴国平曹雪潘永飞张振超秦文斌姚悦梅
Owner ZHENJIANG AGRI SCI INST JIANGSU HILLY AREAS
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