Novel cation lipoid, preparation and use thereof

A cationic lipid and solid lipid technology, applied in the chemical field, can solve the problems of high toxicity, affecting cell resistance to gene transfection, etc., and achieve the effects of small particle size, improved gene therapy effect, and strong binding ability
CN101475503AInactive Publication Date: 2009-07-08SHANDONG UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
SHANDONG UNIV
Publication Date
2009-07-08
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to a method for designing and synthesizing novel single-chain cationic lipids and application of the novel single-chain cationic lipids in preparing a nuclear gene nano-carrier by adoption of the novel single-chain cationic lipids as a surfactant. The method belongs to the technical field of chemistry, and is characterized in that the single-chain cationic lipids with easy dissolution, high efficiency and low toxicity are prepared by a chemical synthesis method; the synthesis method is simple; and the yield of products is high. Moreover, the novel single-chain cationic lipids are taken as the surfactant and applied in various carrier materials, and the nuclear gene nano-carrier which has uniform particle diameter, high drug loading amount and good stability, and is easy in surface modification is prepared by various methods. When acted on various tumor cells, the prepared nuclear gene nano-carrier shows lowcytotoxicity and high transfection efficiency. The lipids have good stability, high surface activity and good biodegradability, and can be used for preparing various nuclear gene nano-carriers.
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Description

technical field

[0001] The invention relates to a novel single-chain cationic lipid, its preparation method and its application in gene-charged nano-carriers, and belongs to the field of chemical technology. Background technique

[0002] Gene therapy is a hot spot in modern cancer treatment, and it is gradually becoming a reality. The main goal of gene therapy is the successful delivery of genetic material to target tissues, organs or cells. However, naked therapeutic genes are easily degraded by nucleases, showing poor cellular uptake efficiency. Therefore, the preparation of safe and efficient gene carriers has become a necessary condition for successful gene therapy.

[0003] Viral vectors can effectively transfect genetic material into host cells, but they have many disadvantages, such as easy carcinogenesis, mutagenicity, and the possibility of inducing immune responses to make transgene expression disappear. Therefore, more and more people have turned their attentio...

Claims

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