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CAPs (C1eaved Amplified Polymorphic Sequences) molecular marker for identifying amaranthus tuberculatus based on SNP (Single Nucleotide Polymorphism) site and application thereof

A technology of molecular markers and amaranthus, which is applied in the fields of genetic engineering and molecular biology, can solve the problems of inability to further identify and achieve accurate detection and identification

Inactive Publication Date: 2017-03-15
CHINESE RES ACAD OF ENVIRONMENTAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most plants of the genus Amaranthus can be distinguished by the ITS sequence (629bp), but the Western Amaranth, Amaranthus amaranth, and Amaranth complex group cannot be further identified
Species identification can only be performed based on SNP sites within the sequence

Method used

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  • CAPs (C1eaved Amplified Polymorphic Sequences) molecular marker for identifying amaranthus tuberculatus based on SNP (Single Nucleotide Polymorphism) site and application thereof
  • CAPs (C1eaved Amplified Polymorphic Sequences) molecular marker for identifying amaranthus tuberculatus based on SNP (Single Nucleotide Polymorphism) site and application thereof
  • CAPs (C1eaved Amplified Polymorphic Sequences) molecular marker for identifying amaranthus tuberculatus based on SNP (Single Nucleotide Polymorphism) site and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1 is used to identify the acquisition of CAPs molecular markers of Amaranth

[0025] 1. Plant materials The specific names and sources of the materials selected in this example are shown in Table 1. The plant materials used in the experiment were leaves and seeds dried on silica gel.

[0026] Table 1 Plant materials and sources

[0027]

[0028]

[0029] 2. Reagents The reagents used for PCR amplification were purchased from Dalian Bao Biological Co., Ltd., the primers were synthesized by Beijing Aoke Dingsheng Biotechnology Co., Ltd., and the sequencing was completed by Shanghai Bioengineering Technology Service Co., Ltd.

[0030] 3. Method

[0031] 3.1 DNA extraction

[0032]Put 100mg of silica gel-dried plant leaves or seeds into a 2ml EP tube with 4mm steel balls added in advance, quickly freeze in liquid nitrogen for 30min, place the EP tube on a Geno / Grinder2000 (SPEX SamplePrep) high-throughput grinder, and grind for 1.5min. 1000rpm / min. Total l...

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Abstract

The invention provides a CAPs (C1eaved Amplified Polymorphic Sequences) molecular marker for identifying amaranthus tuberculatus based on an SNP (Single Nucleotide Polymorphism) site and application thereof. The molecular marker is located on ITS (Internal Transcribed Spacers) and 26S sequences of an amaranthaceous plant nuclear gene, and a nucleotide sequence of the molecular marker is as shown in SEQ ID NO.1. Primer pair sequences for amplifying the molecular marker are as shown in SEQ ID NO.2 and SEQ ID NO.3. The specific molecular marker provided by the invention can realize rapid and accurate detection of the amaranthus tuberculatus and a reliable detection method is provided for rapid distinguishing and identification of alien invasive species.

Description

technical field [0001] The invention relates to the fields of genetic engineering and molecular biology, in particular to a CAPs molecular marker for identifying amaranth and its application. Background technique [0002] The invasive plants Amaranthus amaranthus and Western Amaranthus belong to Amaranthaceae Amaranthus genus Amaranthus genus, native to North America, and are super weeds in soybean and corn farmland in the United States. In recent years, with the increase in trade between China and the United States, the risk of importing grain and other goods into my country has increased, and they have been intercepted at Chinese ports many times. The seeds of amaranth and western amaranth are small, only 0.6-0.8mm, and they are similar in shape, so it is difficult to distinguish them. Hybridization between species often occurs, resulting in a series of polymorphic complex groups, which is a difficult problem in port detection and identification and plant taxonomy. Rober...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6895C12Q2600/156Y02A90/40
Inventor 徐晗赵彩云刘勇波李俊生
Owner CHINESE RES ACAD OF ENVIRONMENTAL SCI
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