Method for culturing sweet pepper cenospecies by genic male sterility dual purpose lines

Abstract

The method of breeding sweet pepper hybrid with nuclear two-purpose male sterility line of sweet pepper features the breeding process, which includes the cross-breeding, selfing and sib breeding process to transfer the male sterile nuclear gene (msms) into selfing sweet pepper line with excellent agricultural character to obtain nuclear two-purpose male sterility line of sweet pepper of sterile plant rate of 50 %; the agricultural character selectivity of sib breeding for 2 or 3 generations to selectively breed the two-purpose male sterility line of sweet pepper with stable agricultural character, stable sterile plant rate of about 50 % and sterile degree of 100 %; culturing the hybrid seed with the sterile plants of the nuclear two-purpose male sterility line as female parent and the selfing line as male parent, removing the breeding plants before pollination and collecting seeds of the female parent. The present invention uses the nuclear male sterility line as both sterile line and maintainer line to simplify the breeding process.

Description

technical field [0001] The invention relates to a method for cultivating crop varieties, in particular to a method for cultivating hybrids by using a male sterile dual-purpose line of sweet pepper. Background technique [0002] The heterosis of sweet pepper is significant, so cultivating hybrids is an effective way to increase yield, improve quality and enhance stress resistance. Present method for cultivating sweet pepper hybrids: 1, utilizing inbred lines to hybridize and breed hybrids. When using this method for hybrid seed production, manual detasseling at the bud stage is required. Because the sweet pepper flowers are small and tender, manual detasselling at the bud stage is difficult, requires strong technology, requires a lot of labor, high cost, and is difficult to guarantee the purity. 2. Patent 02125723.X discloses a method for seed production using cytoplasmic male sterile lines of capsicum or sweet pepper. Although this method overcomes the shortcomings of the a...

AI Technical Summary

Problems solved by technology

When using this method for hybrid seed production, manual detasseling at the bud stage is required. Because the bell peppers are small and tender, manual detasseling at the bud stage is difficult, requires strong technology, requires a lot of labor, high cost, and the purity is difficult to guarantee.

2. Patent 02125723.X discloses a method for seed production using cytoplasmic male sterile lines of capsicum or sweet pepper. Although this method overcomes the shortcomings of the above-mentioned inbred line breeding hybrids to a certain extent, it uses The male sterility of the cytoplasmic sterile line is controlled by the nuclear and cytoplasmic genes, and the use of the cytoplasmic sterile line to breed hybrids requires not only the selection of male sterile lines, but also the selection of maintainer lines, and then breeding Only when the restorer line reaches the three-line matching can it be cultivated into a hybrid. The breeding procedure is cumbersome and takes a long time; The breeding and preservation procedures of the line itself are also cumbersome