Method for culturing sweet pepper cenospecies by genic male sterility dual purpose lines

A male sterility, dual-purpose line technology, applied in the directions of botanical equipment and methods, applications, plant genetic improvement, etc., can solve the problems of cumbersome reproduction and preservation procedures, difficulty in hybridization, and difficulty in ensuring purity, and avoid mechanical damage. Column head, not easily affected by environmental conditions, the effect of ensuring purity

Inactive Publication Date: 2005-08-17
河北省农林科学院经济作物研究所
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AI Technical Summary

Problems solved by technology

When using this method for hybrid seed production, manual detasseling at the bud stage is required. Because the bell peppers are small and tender, manual detasseling at the bud stage is difficult, requires strong technology, requires a lot of labor, high cost, and the purity is difficult to guarantee.
2. Patent 02125723.X discloses a method for seed production using cytoplasmic male sterile lines of capsicum or sweet pepper. Although this method overcomes the shortcomings of the above-mentioned inbred line breeding hybrids to a ce...

Method used

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Embodiment Construction

[0019] 1. Transgenesis of male sterile dual-purpose lines of sweet pepper

[0020] Take the nuclear male sterile line AB of the Institute of Economic Crops, Hebei Academy of Agriculture and Forestry Sciences 91 It is a source of sterility, and its shape is a red square lantern. The red square lantern inbred line S909 is used as the target parent for transduction, and the nuclear male sterility gene (msms) is transferred by crossing, selfing, and sister crossing. In the S909 sweet pepper inbred line, the selection of agronomic traits was carried out through two generations of sister crossing at the same time, and two male sterile sweet peppers with stable agronomic traits, a stable sterile plant rate of about 50%, and a sterility degree of 100% were selected. Use AB 91-S909-3-2 .

[0021] 2. Propagation of seeds of the nuclear male sterile line

[0022] Disinfect the seeds of the nuclear male sterile dual-purpose line first: soak the seeds in 55°C warm water for 20-30 minute...

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Abstract

The method of breeding sweet pepper hybrid with nuclear two-purpose male sterility line of sweet pepper features the breeding process, which includes the cross-breeding, selfing and sib breeding process to transfer the male sterile nuclear gene (msms) into selfing sweet pepper line with excellent agricultural character to obtain nuclear two-purpose male sterility line of sweet pepper of sterile plant rate of 50 %; the agricultural character selectivity of sib breeding for 2 or 3 generations to selectively breed the two-purpose male sterility line of sweet pepper with stable agricultural character, stable sterile plant rate of about 50 % and sterile degree of 100 %; culturing the hybrid seed with the sterile plants of the nuclear two-purpose male sterility line as female parent and the selfing line as male parent, removing the breeding plants before pollination and collecting seeds of the female parent. The present invention uses the nuclear male sterility line as both sterile line and maintainer line to simplify the breeding process.

Description

technical field [0001] The invention relates to a method for cultivating crop varieties, in particular to a method for cultivating hybrids by using a male sterile dual-purpose line of sweet pepper. Background technique [0002] The heterosis of sweet pepper is significant, so cultivating hybrids is an effective way to increase yield, improve quality and enhance stress resistance. Present method for cultivating sweet pepper hybrids: 1, utilizing inbred lines to hybridize and breed hybrids. When using this method for hybrid seed production, manual detasseling at the bud stage is required. Because the sweet pepper flowers are small and tender, manual detasselling at the bud stage is difficult, requires strong technology, requires a lot of labor, high cost, and is difficult to guarantee the purity. 2. Patent 02125723.X discloses a method for seed production using cytoplasmic male sterile lines of capsicum or sweet pepper. Although this method overcomes the shortcomings of the a...

Claims

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Application Information

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IPC IPC(8): A01H1/02
Inventor 范妍芹刘云严立斌
Owner 河北省农林科学院经济作物研究所
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