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Preparation method of transgenic dunaliella salina for improving photosynthesis efficiency

A photosynthesis and transgenic technology, applied in the direction of microorganism-based methods, botany equipment and methods, biochemical equipment and methods, etc., can solve the problems of photosynthetic activity decline, Calvin cycle renewal ability weakening, etc., to achieve efficiency improvement and cost reduction , the effect of accelerating the accumulation

Inactive Publication Date: 2010-08-18
ZHENGZHOU UNIV
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Problems solved by technology

The enzyme activity is slightly inhibited, which will lead to the weakening of Calvin cycle renewal ability and the sharp decline of photosynthetic activity

Method used

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  • Preparation method of transgenic dunaliella salina for improving photosynthesis efficiency
  • Preparation method of transgenic dunaliella salina for improving photosynthesis efficiency
  • Preparation method of transgenic dunaliella salina for improving photosynthesis efficiency

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Embodiment Construction

[0030] The present invention will be further described below in conjunction with examples (taking eukaryotic unicellular microalgae salina as an example), but not limited to salina.

[0031] 1. Salina culture

[0032] 1. Liquid culture: Salina cells were inoculated in Mclachlan culture medium, and the culture conditions were as follows: temperature 25±2°C, light intensity 3000 lux, light culture for 14 hours and dark culture for 10 hours every day.

[0033] 2. Solid culture: Add agar concentration of 0.5-0.8% to the Mclachlan culture medium, use sterilized white gold fungus to inoculate the algae from the liquid culture medium on the surface of the solid medium immediately on the ultra-clean bench, put it in the incubator and cultivate it in the incubator Fluorescent tubes need to be configured for irradiation in the medium, and the culture is carried out according to the requirements of liquid culture conditions.

[0034] 2. Cyanobacteria cultivation

[0035] The algal spec...

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Abstract

The invention provides a preparation method of transgenic dunaliella salina for improving the photosynthesis efficiency, which is characterized by comprising the following steps: colonizing or reforming genes related to carbon fixation and photosynthesis in a photosynthetic autotroph; transferring the genes into a chloroplast genome or nuclear genome of the dunaliella salina for expressing; successively and selectively culturing; and finally obtaining a stable transgenic dunaliella salina strain. By introducing independent or combined expression of high-efficiency genes related to carbon fixation and photosynthesis, the invention improves the concentration and the transferring speed of CO2 in the transgenic dunaliella salina and ensures that the capability of the transgenic dunaliella salina using the CO2 for photosynthesis can be improved, thereby promoting the autotrophic growth of the transgenic dunaliella salina, accelerating and increasing the accumulation of biomass liveweight of dunaliella salina cells and meeting the requirement on scale production of biodiesel and metabolin thereof as well as genetic engineering products.

Description

technical field [0001] The present invention relates to microalgae genetic engineering, which is a method for preparing transgenic Salina salina for improving photosynthesis efficiency, specifically through cloning or modifying carbon fixation and photosynthesis-related genes of photoautotrophs, and introducing them into Salina salina genome for expression alone or in combination , to improve the utilization of CO by Salina 2 The ability to carry out photosynthesis, accelerate and improve the accumulation of salina cell biomass, and meet the large-scale production requirements of salina biodiesel, salina metabolites and genetic engineering products under open culture conditions. Background technique [0002] Extensive use of petroleum has led to energy depletion and increased greenhouse gas emissions. In order to achieve harmonious development of the economy and the environment, renewable energy must be used to replace petroleum and reduce greenhouse gas emissions. Protectin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/52C12N15/29C12N15/63C12N1/13C12R1/89
Inventor 潘卫东薛乐勋李杰
Owner ZHENGZHOU UNIV
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