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Kit for detecting stomach cancer inheritance risk

A kit and gastric cancer technology, applied in the field of molecular biology, can solve the problems of inability to mediate the detoxification effect of glutathione, increase the risk of gastric cancer, gene deletion, etc.

Inactive Publication Date: 2013-10-30
解码(上海)生物医药科技有限公司
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  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

The entire gene deletion of GSTM1 cannot mediate the detoxification effect of glutat

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1. Use of detection kits

[0027] 1. Extract DNA template

[0028] The epithelial cells of the oral mucosa of the subjects were scraped, and the genomic DNA was extracted by the phenol-chloroform method.

[0029] 2. PCR amplification reaction

[0030] Use the PCR reaction component in the detection kit, which contains the following primer pairs:

[0031] (1) MTHFR (C677T) forward primer: 5'CATCCCTCGCCTTGAACAG3'

[0032] MTHFR (C677T) reverse primer: 5'CAGACACTGTTGCTGGGTTTT3'

[0033] (2) TNF-β (A252G) forward primer: 5'CAGAAGGAGGAGGTGTAGGGT3'

[0034] TNF-β (A252G) reverse primer: 5'TTCGTGCTTTGGACTACCG 3'

[0035] (3) TNF-α (G-308A) forward primer: 5'GGAAGCCAAGACTGAAACCA3'

[0036] TNF-α (G-308A) reverse primer: 5' TCATCTGGAGGAAGCGGTAG 3'

[0037] (4) hMLH1 (T1151A) forward primer: 5'ACAGACTTTGCTACCAGGACTTG3'

[0038] hMLH1 (T1151A) reverse primer: 5'TGTCTTATTCCTCTGTGACAATG3'

[0039] The reaction system for PCR amplification is: 10×PCR reacti...

Embodiment 2

[0055] Example 2. Services for non-invasive detection of gastric cancer genes for the population

[0056] 1. Sampling and DNA extraction

[0057] The physicians in the laboratory department of the hospital will guide the subjects to use oral swabs to sample oral epithelial cells, and use the phenol-chloroform method to extract DNA from oral epithelial cells

[0058] 2. Genotype detection

[0059] Using the kit provided by the present invention, four single nucleotide polymorphisms of C677T on the MTHFR gene, A252G on the TNF-β gene, G-308A on the TNF-α gene, and T1151A on the hMLH1 gene of the subject’s genomic DNA The loci were subjected to DNA sequencing to determine the genotypes of the 4 SNPs loci.

[0060] 3. Risk assessment and analysis of gastric cancer high-risk groups

[0061] Through the analysis of the SNPs genotype of the subjects, a gastric cancer risk assessment and analysis report is issued. The report details the SNP site genetic detection information of C6...

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Abstract

The invention provides a kit for detecting stomach cancer inheritance risk. The kit comprises specific primers and DNA (deoxyribonucleic acid) sequencing primers for detecting genotypes of 4 mononucleotide polymorphism sites (C677T on MTHFR gene, A252G on TNF-beta gene, G-308A on TNF-alpha gene and T1151A on hMLH1 gene), a PCR (polymerase chain reaction) assembly, a PCR product purification assembly, a DNA sequencing reaction assembly and the like. The kit provided by the invention detects genotypes of GSTM1 gene deficiency polymorphism and 4 mononucleotide polymorphism sites closely related to stomach cancer to evaluate the attack risk level of stomach cancer, thereby preventing the generation of stomach cancer. The sampling method is an oral mucosa cell sampling method which is painless and noninvasive and avoids cross infection. The method has the advantage of accurate and reliable sequencing detection result, does not need to buy expensive imported special instruments, and thus, is easy to popularize.

Description

technical field [0001] The invention belongs to the field of molecular biology, relates to medicine and biotechnology, and specifically relates to a gastric cancer gene detection kit, which evaluates the risk level of gastric cancer in a population from the genetic level according to the detection results, and guides early prevention and treatment of gastric cancer high-risk groups. Background technique [0002] There are more than 1 million new cases of gastric cancer every year in the world, China accounts for 42%, and about 800,000 deaths, China accounts for 35%, is one of the countries with the highest incidence and mortality of gastric cancer, and the incidence and mortality are more than twice the world average . In 2011, Chen Wanqing and others pointed out in the "Analysis of Cancer Incidence and Death in China's Cancer Registration Areas in 2007" that: In 2007, among the top 10 cancers in my country, (1) the incidence rate of gastric cancer was the second, and the m...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 李为为姜丽
Owner 解码(上海)生物医药科技有限公司
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