Genetic engineering polypeptide vaccine adjuvant for aftosa, preparation method and application thereof

A polypeptide vaccine and genetic engineering technology, applied in the field of genetic engineering, can solve problems such as weak immunogenicity

Inactive Publication Date: 2007-07-11
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the immunogenicity of genetically engineered vaccines is relatively weak. In order to better stimulate the immune system of the body and make it have sufficient resistance to foot-and-mouth disease virus (FMDV), it is necessary to seek a more convenient and effective method to improve the efficacy of genetically engineered polypeptide vaccines. immune activity

Method used

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Examples

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Comparison scheme
Effect test

Embodiment Construction

[0038] Hereinafter, the present invention will be further specifically described by taking the porcine foot-and-mouth disease O-type genetically engineered polypeptide vaccine and the novel adjuvant involved in the present invention as examples.

[0039] Adjuvant construction process. Obtain domestic pig liver samples and use total RNA extraction reagent (Trizol, product of Invitrogen Company) to extract liver total RNA, obtain from domestic pig liver total RNA by RT-PCR method, including 23 signal peptides and a full length of 189 The domestic pig IFN-α gene of amino acid, its nucleotide sequence is SEQ.ID.NO.2, and the aminoacid sequence of its coding is SEQ.ID.NO.1, above-mentioned gene is inserted into prokaryotic cloning vector pMD18-T (TaKaRa company coli TG1, and the positive clones were selected for sequencing and compared with the sequence of domestic pig IFN-α provided by GenBank to confirm that the correct gene was obtained; the above gene was inserted into eukaryot...

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Abstract

The invention relates to a method for preparing polypeptide vaccine agent used in foot-and-mouth disease gene project, wherein said agent is recombined particle that prepared by colonizing pig interferon-alpha gene via molecule biological technique into eucaryon expression carrier; the said agent is recombined particle that prepared by colonizing pig is one of interferon-alpha gene group; the eucaryon expression carrier is any DNA expression carrier with eucaryon starter, protocaryon copier, and protocaryon resistant gene; said inventive particles will transfer into bacillus coli to expand, via alkali crack method to obtain many particles, to be diluted by PBS buffer liquid into some density, to prepare inventive polypeptide vaccine agent. Animal test has proved that said agent is safe coupled with O-tyep vaccine of foot-and-mouth disease gene project, while the protection ration can reach 100% by one injection.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, in particular to a foot-and-mouth disease genetic engineering polypeptide vaccine adjuvant and a preparation method and application thereof. Background technique [0002] Foot-and-mouth disease (FMD) is one of the most serious livestock infectious diseases in the world today, mainly harming pigs, cattle, sheep and other cloven-hoofed animals, and has a wide range of outbreaks, rapid spread, and high mortality after infection , causing huge economic losses to the animal husbandry industry, and thus was listed as the first class A infectious disease by the International Veterinary Bureau. [0003] Genetic engineering polypeptide vaccines have broad application prospects due to their advantages of good safety, easy storage and ideal effect. However, the immunogenicity of genetically engineered vaccines is relatively weak. In order to better stimulate the immune system of the body and ma...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K48/00A61K39/39A61K39/135C12N15/79C12N15/21A61P31/14
Inventor 郑兆鑫严维耀程功刘明秋
Owner FUDAN UNIV
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