Tissue engineering bracket material capable of physically embedding active substances and preparation method thereof

A tissue engineering scaffold and active substance technology, applied in the field of biological scaffold materials and its preparation, can solve problems such as unfavorable industrialization and clinical application, changes in cell phenotype, gene and function, and bacterial contamination of cells

Inactive Publication Date: 2012-07-11
汪泱
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, because tissue engineering involves more than 8 weeks of autologous cell isolation and culture, tissue construction and culture in vitro, traditional tissue engineering research methods are not universal and restrict industrialization and clinical application.
In addition, long-term cell culture and in vitro tissue construction may lead to changes in cell phenotype, gene and function, and the probability of cell contamination by bacteria is greatly increased. These problems are also extremely unfavorable to its industrialization and clinical application

Method used

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  • Tissue engineering bracket material capable of physically embedding active substances and preparation method thereof
  • Tissue engineering bracket material capable of physically embedding active substances and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Preparation of polymer spinning solution: polycaprolactone (PCL, molecular weight 100,000), gelatin (GE, molecular weight 100,000) and active substances (fibroblast growth factor bFGF, endothelial cell growth factor VEGF or a certain proportion of rich Platelet plasma (PRP) was dissolved in trifluoroethanol and prepared into a mixed solution with a total concentration of 12w / v% as a polymer spinning solution, wherein the mass ratio of PCL, GE and active substances was 70:25:5.

[0034] Preparation of scaffold material: select a single-nozzle spinning device, put the obtained polymer spinning liquid into the liquid storage device of the electrospinning equipment, adjust the feeding rate of the solution to 20ul / min, and the spinneret and the grounded collector The distance between them is 12cm; the ambient temperature is 25°C; the static voltage is 20kV, and polymer nanofibers are obtained on the collector with an average fiber diameter of 400nm.

[0035] Soak the obtaine...

Embodiment 2

[0038] The preparation of polymer spinning solution: polycaprolactone (PCL, molecular weight 100,000) is dissolved in the mixed solvent of chloroform and methanol of 80:20, is mixed with the PCL solution of 8w / v%; Gelatin (GE, molecular weight 300,000) and active substances (bone morphogenic protein BMP-2, transforming growth factor TGF-β or a certain proportion of platelet-rich plasma PRP) were dissolved in water to prepare a mixed solution with a total concentration of 15w / v%, wherein GE and active The mass ratio of substances was 90:10.

[0039] Preparation of stent material: use a double-nozzle spinning device, put the PCL solution prepared above and the GE mixed solution containing active substances into two different infusion channels respectively, adjust the feeding rate of the solution to 15ul / min, and spin The distance between the head and the grounded collector is 12cm; the ambient temperature is 25°C; the electrostatic voltage of PCL electrospinning is 18kV, the ele...

Embodiment 3

[0042] Preparation of polymer spinning solution: dissolve polycaprolactone (PCL, molecular weight 100,000) and active substances (endothelial cell growth factor VEGF, platelet-rich plasma PRP) in a mixed solvent of 80:20 chloroform and methanol, and prepare 8w / v% spinning solution, wherein the mass ratio of PCL and active material is 85:15.

[0043] Preparation of scaffold material: select a single-nozzle spinning device, put the obtained polymer spinning liquid into the liquid storage device of the electrospinning equipment, adjust the feeding rate of the solution to 20ul / min, and the spinneret and the grounded collector The distance between them is 12cm; the ambient temperature is 25°C; the static voltage is 20kV, and polymer nanofibers with a thickness of 0.4mm are obtained on the collector, and the average diameter of the fibers is 500nm.

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Abstract

The invention relates to a tissue engineering bracket material capable of physically embedding active substances and a preparation method thereof. The tissue engineering bracket material capable of physically embedding the active substances is prepared from 100 weight parts of biodegradable macromolecular substance and 0 to 20 weight parts of active substances, wherein the weight part of the active substances is not equal to zero; 100 weight parts of biodegradable macromolecular substance consists of 50 to 100 weight parts of synthetic macromolecular substance and 0 to 50 weight parts of natural macromolecular substance; the macromolecular substances and the active substances form a micromolecular nanofiber membrane or a composite macromolecular nanofiber membrane; and the active substances are bonded on the surface of the fiber membrane uniformly. The tissue engineering bracket material capable of physically embedding the active substances has high biocompatibility and sufficient mechanical strength, can be biodegraded, is suitable for proliferation and directed differentiation of stem cells, and has the functions of promoting cell migration adhesion and capturing the stem cells so as to induce regeneration of tissues such as bones, cartilages, nerves, skin and the like.

Description

technical field [0001] The invention relates to a biological material, in particular to a biological scaffold material capable of trapping stem cells, inducing stem cell proliferation and directional differentiation, and inducing tissue regeneration, as well as its preparation method and medical application. Background technique [0002] The main idea of ​​tissue engineering is to design and prepare three-dimensional biomimetic tissue, construct a biomaterial scaffold with structure and function similar to natural extracellular matrix, and provide an ideal microenvironment for cell growth, development and intercellular communication in vitro. [0003] Electrospinning technology can continuously prepare nanoscale or submicron nanofibers, and the scaffolds constructed by it have high porosity, good channel connectivity and high specific surface area, which is a hot research topic at home and abroad. By adjusting the concentration of the solution and the parameters of the spinn...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/26A61L27/24A61L27/22A61L27/20A61L27/36A61L27/18A61L27/54
Inventor 汪泱聂华荣邓志锋易应萍郭菲
Owner 汪泱
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