The invention relates to a fertilizer, and in particular discloses a seaweed biological fertilizer and a preparation method thereof. The seaweed biological fertilizer is made of the following raw materials in parts by weight: 30-50 parts of seaweed, 1-3 parts of hoof horn powder, 1-4 parts of EM bacteria, 2-3 parts of amino acid powder, and 1-4 parts of potassium humate , 12-14 parts of soybean powder, 12-35 parts of vitamin C, 2-6 parts of plant ash, 2-4 parts of Gongluomu, 4-8 parts of Smilax smilax, 1-3 parts of saponins, 2-5 parts of Toosendan, 3‑5 parts of Eucalyptus leaves, 3‑5 parts of Striga, 3‑6 parts of Cnidium, 4‑6 parts of Nepeta, 3‑8 parts of Morning Glory, and 3‑5 parts of Astragalus. Beneficial effects of the present invention: rich in organic matter, high utilization rate of fertilizer efficiency, long duration of fertilizer effect, comprehensive fertilizer nutrition, beneficial to soil improvement, fast absorption of crops, convenient application, both seaweed activity and living body regulation function of biological bacteria agent, can Effectively promote high yield and early maturity of crops, improve disease resistance and improve crop quality.