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A kind of seaweed bacterial fertilizer containing seaweed extract and preparation method thereof

A technology of extracting liquid and seaweed, which is applied in the field of seaweed bacterial fertilizer and its preparation, can solve the problems of single function and poor active ingredients, and achieve the effects of fast fertilizer effect, outstanding effect, and improved disease resistance

Active Publication Date: 2014-10-29
QINGDAO BRIGHT MOON SEAWEED GROUP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Although the above-mentioned seaweed fertilizers are rich in nutrients, their active ingredients are poor, their functions are single, and their application fields still have certain limitations.

Method used

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  • A kind of seaweed bacterial fertilizer containing seaweed extract and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] (1) Extraction of Ascophyllum nodosum

[0038] Take 40 kg of Ascophyllum nodosum, crush it to 80 mesh, and sieve it for later use; preheat the deionized water with pH 7.0-7.5 to 30°C, add 400kg of water according to the ratio of Ascophyllum nodosum: water = 1:10 (weight ratio) , fully stirred, extracted for 15 hours, the extract was centrifuged at 3000rpm for 20 minutes, and the supernatant was taken, which was the extract of Ascophyllum nodosum, and set aside;

[0039] (2) Preparation of fermentation medium

[0040] Take 100kg of Ascophyllum nodosum extract obtained in step (1), add 1kg of peptone (1%, W / W), yeast extract (0.5%, W / W) 0.5kg; sodium chloride (1%, W / W) 0.5kg; KH 2 PO 4 (0.1%W / W) 0.1kg; MgSO 4 (0.01%, W / W) 0.05kg; use ammonia water to adjust the pH of the medium to 7.0;

[0041] (3) Activation of Bacillus subtilis BS-1 seeds

[0042] The Bacillus subtilis BS-1 (preservation number is CGMCC No.6616) was transferred from the preserved slant to the fres...

Embodiment 2

[0047] (1) Spirulina extraction

[0048] Take 40 kg of blue-green algae, crush it to 120 mesh, and sieve it for later use; preheat the deionized water to 30°C, add 1000 kg of water according to the ratio of blue-green algae: water = 1:25 (weight ratio), stir well, extract for 2 hours, and extract The extract was centrifuged at 2000rpm for 30 minutes, and the supernatant was taken, which was the cyanobacteria extract, which was set aside;

[0049] (2) Preparation of fermentation medium

[0050] Take 100kg of cyanobacteria extract obtained in step (1), add 0.6kg peptone (1%, W / W), yeast extract (0.5%, W / W) 0.1kg; sodium chloride (1%, W / W) 1kg ;KH 2 PO 4 (0.1%W / W) 0.05kg; MgSO 4 (0.01%, W / W) 0.01kg; use ammonia water to adjust the pH of the medium to 8.0;

[0051] (3) Activation of Bacillus subtilis BS-1 seeds

[0052] The Bacillus subtilis BS-1 (preservation number is CGMCC No.6616) was transferred from the preserved slant to the fresh test tube slant, and cultured in a co...

Embodiment 3

[0057] (1) Kelp extraction

[0058] Take 40 kg of kelp, crush it to 100 mesh, and sieve it for later use; preheat the deionized water to 35°C, add 800 kg of water according to the ratio of kelp: water = 1:20 (weight ratio), stir well, extract for 8 hours, and The extract is centrifuged at 2500rpm for 20 minutes, and the supernatant is taken, which is the kelp extract, which is set aside;

[0059] (2) Preparation of fermentation medium

[0060] Take 100kg of kelp extract obtained in step (1), add 0.8kg peptone (1%, W / W), yeast extract (0.5%, W / W) 0.3kg; sodium chloride (1%, W / W) 0.7 kg; KH 2 PO 4 (0.1%W / W) 0.08kg; MgSO 4 (0.01%, W / W) 0.03kg; use ammonia water to adjust the pH of the medium to 7.2;

[0061] (3) Activation of Bacillus subtilis BS-1 seeds

[0062] The Bacillus subtilis BS-005 (preservation number is CGMCC No.6616) was transferred from the preserved slant to the fresh test tube slant, and cultured in a constant temperature incubator at 38°C for 24 hours. Tak...

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Abstract

The invention relates to a seaweed fertilizer comprising a seaweed leach liquor. The seaweed fertilizer is prepared by the following steps: (1) seaweed extraction; (2) preparation of a fermentation medium; (3) inoculation and fermentation. The seaweed fertilizer provided by the invention is an active biological fungicide fertilizer of seaweed produced by pure physical and biological methods, and has the activity of seaweed and the living body adjusting function of biological fungicide. The number of effective living bacteria is greater than or equal to 2.0*10 to the power of 9 CFU / ml. Meanwhile, the fertilizer is rich in plant growth-regulating activity substances such as alginic acid, seaweed oligosaccharide, gibberellin (GA) and indoleacetic acid (IAA) biologically fermented, degraded and converted from seaweed gel. The fertilizer is directly absorbed by root systems of crops conveniently and fast in fertilizer efficiency, and can effectively accelerate high yield and early-ripe of plants, enhance the disease-resistance and improve the quality of crops, so that the fertilizer is an excellent special fertilizer for organic food.

Description

technical field [0001] The invention relates to a seaweed fertilizer containing seaweed extract and a preparation method thereof, belonging to the field of organic biological fertilizers. Background technique [0002] Seaweed (Alga) is algae that grows in the sea and is a cryptogamous plant in the plant kingdom. They are generally considered to be simple plants, and the main features are: no vascular tissue, no real differentiation of roots, stems, and leaves; no flowering, no fruits and seeds; no specialized protective tissues for reproductive organs, often directly formed by a single Cells producing spores or gametes; and no embryo formation. Because of the simple structure of algae, some botanists attribute it to the "phylloid flora" of lower plants along with fungi. [0003] Seaweed is a lower plant with a wide variety. According to the different pigments contained in seaweed, it can be divided into green algae (such as Ulva), brown algae (such as kelp, sargassum, sea...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C05F11/08
Inventor 李同玉周波李园园赵宏涛张铭铄
Owner QINGDAO BRIGHT MOON SEAWEED GROUP
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