Seaweed fertilizer comprising seaweed leach liquor and preparation method thereof
A technology of extracting liquid and seaweed is applied in the field of seaweed bacterial fertilizer and its preparation, which can solve the problems of poor active ingredients and single function, and achieve the effects of outstanding effect, fast fertilizer effect and high yield promotion.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0037] (1) Extraction of Ascophyllum nodosum
[0038] Take 40 kg of Ascophyllum nodosum, crush it to 80 mesh, and sieve it for later use; preheat the deionized water with pH 7.0-7.5 to 30°C, add 400kg of water according to the ratio of Ascophyllum nodosum: water = 1:10 (weight ratio) , fully stirred, extracted for 15 hours, the extract was centrifuged at 3000rpm for 20 minutes, and the supernatant was taken, which was the extract of Ascophyllum nodosum, and set aside;
[0039] (2) Preparation of fermentation medium
[0040] Take 100kg of Ascophyllum nodosum extract obtained in step (1), add 1kg of peptone (1%, W / W), yeast extract (0.5%, W / W) 0.5kg; sodium chloride (1%, W / W) 0.5kg; KH 2 PO 4 (0.1%W / W) 0.1kg; MgSO 4 (0.01%, W / W) 0.05kg; use ammonia water to adjust the pH of the medium to 7.0;
[0041] (3) Activation of Bacillus subtilis BS-1 seeds
[0042] The Bacillus subtilis BS-1 (preservation number is CGMCC No.6616) was transferred from the preserved slant to the fres...
Embodiment 2
[0047] (1) Spirulina extraction
[0048] Take 40 kg of blue-green algae, crush it to 120 mesh, and sieve it for later use; preheat the deionized water to 30°C, add 1000 kg of water according to the ratio of blue-green algae: water = 1:25 (weight ratio), stir well, extract for 2 hours, and extract The extract was centrifuged at 2000rpm for 30 minutes, and the supernatant was taken, which was the cyanobacteria extract, which was set aside;
[0049] (2) Preparation of fermentation medium
[0050] Take 100kg of cyanobacteria extract obtained in step (1), add 0.6kg peptone (1%, W / W), yeast extract (0.5%, W / W) 0.1kg; sodium chloride (1%, W / W) 1kg ;KH 2 PO 4 (0.1%W / W) 0.05kg; MgSO 4 (0.01%, W / W) 0.01kg; use ammonia water to adjust the pH of the medium to 8.0;
[0051] (3) Activation of Bacillus subtilis BS-1 seeds
[0052] The Bacillus subtilis BS-1 (preservation number is CGMCC No.6616) was transferred from the preserved slant to the fresh test tube slant, and cultured in a co...
Embodiment 3
[0057] (1) Kelp extraction
[0058] Take 40 kg of kelp, crush it to 100 mesh, and sieve it for later use; preheat the deionized water to 35°C, add 800 kg of water according to the ratio of kelp: water = 1:20 (weight ratio), stir well, extract for 8 hours, and The extract is centrifuged at 2500rpm for 20 minutes, and the supernatant is taken, which is the kelp extract, which is set aside;
[0059] (2) Preparation of fermentation medium
[0060] Take 100kg of kelp extract obtained in step (1), add 0.8kg peptone (1%, W / W), yeast extract (0.5%, W / W) 0.3kg; sodium chloride (1%, W / W) 0.7 kg; KH 2 PO 4 (0.1%W / W) 0.08kg; MgSO 4 (0.01%, W / W) 0.03kg; use ammonia water to adjust the pH of the medium to 7.2;
[0061] (3) Activation of Bacillus subtilis BS-1 seeds
[0062] The Bacillus subtilis BS-005 (preservation number is CGMCC No.6616) was transferred from the preserved slant to the fresh test tube slant, and cultured in a constant temperature incubator at 38°C for 24 hours. Tak...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com