Batch preparation method of high-purity salvianolic acid A

Abstract

The invention relates provides a batch preparation method of high-purity salvianolic acid A based on physical and chemical properties and chromatograph behavior of a chemical component in salvia miltiorrhiza as well as adaptability of salvia miltiorrhiza to a cell separation technology. In the method, a salvianolic acid B transformation liquid containing salvianolic acid A is subjected to three-time chromatograph purification, namely hyphenated chromatography, reversed-phase chromatography and normal-phase silica gel chromatography, wherein the hyphenated chromatography refers to that macroporous resin frontal chromatography and macroporous resin displacement chromatography are jointly used. The product prepared by the method is controllable in quality, is acceptable in cost and can be used for injection medicaments. The content of salvianolic acid A prepared by the method is more than or equal to 98%, the total recovery of salvianolic acid A is more than or equal to 10% (based on salvianolic acid B), and the total yield of salvianolic acid A is more than or equal to 0.5% (based on salvianolic acid B).

Description

technical field [0001] The invention relates to a batch preparation method of high-purity salvianolic acid A. Background technique [0002] Salvia miltiorrhiza is the basic treatment drug for cardiovascular and cerebrovascular diseases in my country. Due to its definite curative effect, Danshen has become the traditional Chinese medicine with the largest consumption (15,000 tons / year), the highest sales, and the largest number of preparation factories in China (960 preparation factories, and the main dosage forms are: Danshen Dropping Pills, Danshen Injection, Danshen Tablets, Danshen Granules, Danshen Granules and Danshen Capsules), in addition, Danshen is also compounded with other medicinal materials for pulmonary heart disease, hyperlipidemia, hyperviscosity syndrome, asthma, bronchopneumonia, Hepatitis, liver cirrhosis, nephrotic syndrome, arthritis, diabetes, circulatory disorders, various bacterial and viral infections, anti-endotoxin, tumor, scleroderma, dermatitis, ps...

AI Technical Summary

Problems solved by technology

[0013] Although membrane separation technology can effectively remove pseudo-colloidal impurities, because it is a non-tangential force separation technology, the target compound often has a very high affinity with this type of impurity, resulting in serious loss of the target compound

In addition, when membrane technology is used for the separation of such impurities, it also faces the problems of membrane pore clogging and membrane life.

[0014] The selectivity of solvent in solvent extraction technique is very important and the effect is not ideal

[0015] Traditional C 18 Reversed-phase chromatography cannot be widely used due to factors such as small sample volume, limited pH range, inability to perform effective regeneration, short life, and limited cost.

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