Compound microorganism bacterium agent used for sludge reduction, preparation method and application thereof

A compound microbial agent and sludge reduction technology, applied in the direction of sustainable biological treatment, chemical instruments and methods, biological water/sewage treatment, etc., can solve difficult sludge problems and other problems, and promote the increase of the number , strong degradation ability, growth inhibition effect

Active Publication Date: 2012-10-24
河北协同环境科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If we do not control the amount of excess sludge produced and reduce the amount of sludge from the source, but only adopt termin

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The composite microbial agent for sludge reduction is made of Pseudomonas denitrification ATCC 13867, Nocardia coralia CGMCC 4.1037, Candida utilis CGMCC 2.569, Rhodobacter sphaeroides CGMCC 1.2182 and Aspergillus sojae CGMCC 3.661 of liquid bacteria.

[0030] The steps of preparing composite microbial bacterial agent are as follows:

[0031] A. The strains were subjected to liquid amplification culture respectively

[0032] After Pseudomonas denitrifica ATCC 13867 was activated on a slant, it was inoculated in a liquid medium and cultured at a temperature of 30° C. for 48 hours. Liquid medium formula (g / L): sucrose 40, corn steep liquor 20, betaine 5, (NH 4 ) 2 SO 4 1, (NH 4 ) 2 HPO 4 2. MnSO 4 ·H 2 O 0.8, CoC1 2 ·6H 2 O 0.02, MgO 0.3, 5,6-dimethylbenzimidazole (DMBI) 0.01, ZnSO 4 ·7H 2 O 0.01. Adjust the pH of the medium to 7.3.

[0033] After Nocardia coralia CGMCC 4.1037 was activated on a slant, it was inoculated in a liquid medium and cultured at ...

Embodiment 2

[0040] The steps for preparing the compound microbial bacterial agent are as follows:

[0041] A. Liquid scale-up culture

[0042] Three kinds of bacteria of Pseudomonas denitrificans numbered as ATCC 13867, ATCC 15749 and ATCC 19244 were respectively activated on slant surfaces, and then inoculated in liquid medium respectively, and cultured at 28°C for 60 hours. Liquid culture medium formula is with embodiment 1.

[0043] After activation of four strains of Nocardia coralia, the strain numbers CGMCC 4.1037, CGMCC 4.1038, ATCC 19070 and ATCC 19701, were respectively inoculated in liquid medium and cultured at 28°C for 60 hours. Liquid culture medium formula is with embodiment 1.

[0044] Three strains of Candida utilis, numbered CGMCC 2.569, ATCC 8205 and ATCC 9226, were respectively activated on slant surfaces, then inoculated in liquid medium, and cultured at 28°C for 60 hours. Liquid culture medium formula is with embodiment 1.

[0045] Three strains of...

Embodiment 3

[0050] The steps for preparing the compound microbial bacterial agent are as follows:

[0051] A. Liquid scale-up culture

[0052] Pseudomonas denitrifica ATCC 21988 was activated on a slant, inoculated in a liquid medium, and cultured at 26°C for 72 hours. Liquid culture medium formula is with embodiment 1.

[0053] Nocardia coralia ATCC 19148 was activated on a slant, inoculated in a liquid medium, and cultured at 28°C for 72 hours. Liquid culture medium formula is with embodiment 1.

[0054]Candida utilis ATCC 9950 was activated on a slant, inoculated in liquid medium, and cultured at 26°C for 72 hours. Liquid culture medium formula is with embodiment 1.

[0055] Rhodobacter sphaeroides CGMCC 1.2174 was inoculated in liquid medium after being activated on a slant, and cultured at 30°C for 72 hours. Liquid culture medium formula is with embodiment 1.

[0056] After the Aspergillus sojae CGMCC 3.880 was activated on the slant, it was inoculated in the liquid medium and ...

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Abstract

The invention relates to a compound microorganism bacterium agent used for sludge reduction, a preparation method and application of the compound microorganism bacterium agent. The compound microorganism bacterium agent is a liquid bacterium agent prepared by fermenting denitrified pseudomonas, nocardia coralline, candida utilis, rhodobacter sphaeroides and aspergillus sojae. According to the invention, the metabiosis action between microbe consortia is utilized to form a biodegradation system in which the activities of various required enzymes are all high, so dead or decrepit thalli in activated sludge can be effectively damaged and decomposed, and meanwhile, organic matters can be decomposed; and the generation quantity of excess sludge is reduced through reducing the quantity of the sludge in the process without influencing the quality of yielding water. On the premise of not greatly changing the traditional sewage treatment process, the sludge reduction ratio can reach 30%-70% in the sewage treatment process by adopting the compound microorganism bacterium agent, and the comprehensive running cost of the sludge treatment can be reduced, so that the compound microorganism bacterium agent is of great significance on the aspects of economic, environmental and social benefits.

Description

technical field [0001] The invention relates to a microbial agent used in the field of environmental protection, in particular to a microbial agent used for sludge treatment and a preparation method and application technology thereof. Background technique [0002] With the great improvement of my country's urban and industrial sewage treatment capacity, the amount of excess sludge generated in the corresponding sewage treatment process is also increasing day by day, and the annual rate is increasing by 10% to 15%, and the sludge pollution is becoming more and more serious. The problem of sludge treatment highlight. The current sludge in my country is mainly treated in the form of composting, landfilling and random external transportation, which is in a chaotic state. The proportion is roughly 10% for composting, 20% for landfilling, and 70% for simple landfilling and stacking for random external transportation. Even if the current sewage treatment plant has concentrated the ...

Claims

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Application Information

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IPC IPC(8): C02F3/34C02F3/12
CPCY02W10/10
Inventor 卢琼刘洪泉李成义王海波
Owner 河北协同环境科技有限公司
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