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Microbial community and its preparation method and use

A technology of microbial inoculum and strain, applied in the field of microbial inoculum and its preparation, can solve the problems of long fermentation time, high cost, moisture absorption of fermented products, etc., and achieve the effects of simple method, low cost and rapid removal

Inactive Publication Date: 2005-03-16
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0002] At present, there are many patents and literature reports on microbial fermentation agents used in the harmless treatment of livestock and poultry manure, which can be divided into three types: one is the natural flora fermentation method, that is, the accumulation of livestock and poultry manure Under the condition of oxygen, the microorganisms of the waste itself are used for fermentation. The disadvantage of this method is that the fermentation time is long, and the fermentation process will have a certain impact on the environment; the second method is to apply some microorganisms with fermentation function to the feces. Fermentation, but because the functions of high-temperature fermentation strains and deodorization fermentation strains are not used, the fermentation is often incomplete or takes a long time, and the fermented products will return to odor after absorbing moisture; the third method is to use environmentally friendly microbial flora to ferment waste This kind of flora has multiple functions of fermentation, deodorization and killing bacteria, but due to the huge flora and high cost, the application is limited

Method used

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Examples

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Effect test

Embodiment 1

[0011] Cultivation of Yarrowia lipolytica strain: Yarrowia lipolytica is first cultured on a potato medium at 30°C as a primary slant; 48 hours later, it is inoculated into a shaker flask at 30°C as a secondary liquid Shaking culture; after 36 hours, inoculate the inoculum with a weight ratio of 5% on the solid medium, at 30°C, perform tertiary cultivation until the number of bacteria reaches 0.8×10 8 per gram of solid medium.

[0012] Cultivation of Trichoderma viride strains: Trichoderma viride was first cultivated on a PDA medium at 30°C as a primary slant; 72 hours later, it was inoculated into a shaker flask at 30°C for a secondary liquid shaking culture; 48 hours later, Inoculate the inoculum with a weight ratio of 5% on a solid medium, and conduct a tertiary culture at 30°C until the number of bacteria reaches 3.0×10 8 per gram of solid medium.

[0013] Cultivation of hyperthermic bacillus strains: hyperthermic bacillus first cultured on broth peptone medium at 30°C f...

Embodiment 2

[0020] After 1 ton of fresh chicken manure (water content 45~70%) is pretreated by the method in embodiment 1, add 0.05 tons of above-mentioned microbial bacterial agents, ferment at room temperature, after 3 days, temperature can reach 50 ℃, regularly stir and Ventilate, do not let the fermentation temperature exceed 70°C, and the fermentation process will end within 10 days, and a gray fermented product with a slight smell of distiller's grains will be obtained. Contrasted with the natural fermentation group, the results show that adding the microbial inoculum of the present invention shortens the fermentation period from 1.5 months to 1 week; the water content of the product is reduced from 40% to about 20%; the appearance and smell of the product are gray and gray respectively. It has a slight smell of distiller's grains, which is different from the black and musty smell of natural fermentation products; the nitrogen content of the product is 1.5 times that of natural ferme...

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Abstract

The invention discloses a microbiological bacterium and method for making same, wherein the microbiological bacterium comprises degreased saccharomycete, Trichoderma viride, high temperature bacillus, S. melanoferus, Nocardia and solid medium. The microbiological bacterium can be utilized as dejection treatment agent. Its preparation process comprises the three stage pure culture of these bacterial strains, and proportional mixing of the culture.

Description

technical field [0001] The present invention relates to a kind of microbial inoculant and its preparation method and application, more specifically, relate to a kind of yeast that lipolytica (Yaliowia lipolytica), Trichodermaviride (Trichodermaviride), thermobacillus (Bacillus sp. ), Streptomyces pigrifacienfs (Steptomyces pigrifacienfs), Nocardia sp. (Nocardia sp.) five strains and a solid culture medium composed of microbial agents and preparation methods and as the application of feces treatment agent. Background technique [0002] At present, there are many patents and literature reports on microbial fermentation agents used in the harmless treatment of livestock and poultry manure, which can be divided into three types: one is the natural flora fermentation method, that is, the accumulation of livestock and poultry manure Under the condition of oxygen, the microorganisms of the waste itself are used for fermentation. The disadvantage of this method is that the fermentat...

Claims

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Application Information

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IPC IPC(8): C05F3/00C05F11/08C12N1/00C12N1/14C12N1/16C12N1/20
CPCY02A40/20
Inventor 胡国斌丛日山刘怡
Owner OCEAN UNIV OF CHINA
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