Marine actinomycete strain with meloidogyne insecticidal activity and application of marine actinomycete strain

A technology of actinomycetes and strains, applied in the field of microorganisms, can solve problems such as harming human health, easily producing drug resistance, destroying the environment, etc.

Inactive Publication Date: 2016-01-13
INST OF TROPICAL BIOSCI & BIOTECH CHINESE ACADEMY OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The prevention and control measures based on chemical nematicides have many disadvantages, such as: damaging the environment, endangering human health, prone to drug resistance, etc., and are gradually being restricted in use

Method used

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  • Marine actinomycete strain with meloidogyne insecticidal activity and application of marine actinomycete strain
  • Marine actinomycete strain with meloidogyne insecticidal activity and application of marine actinomycete strain
  • Marine actinomycete strain with meloidogyne insecticidal activity and application of marine actinomycete strain

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1: Isolation of bacterial strain HA12420CGMCCNo.11063

[0018] Sediment samples were collected from the mangrove forest in Bamen Bay, Hainan Province. After 7 days of natural air-drying, 5 g of the sample was dissolved in 45 ml of sterile old sea water to make a soil suspension. Dilute gradually to 10 with sterile aged seawater -2 、10 -3 、10 -4 times, draw 100 μl of samples of different dilutions, spread on the actinomycetes isolation medium, and place the isolation plate in a 28°C incubator for upside-down culture. When the colonies grow out, pick colonies with different shapes and colors and streak them on Gaoshi No. 1 synthetic medium for purification until pure culture is obtained. After initial deduplication, number and save.

Embodiment 2

[0019] Embodiment 2: the cultivation of bacterial strain HA12420CGMCCNo.11063

[0020] (1) Incline culture: culture at 28°C for 7 days. Medium: 4.0g of yeast powder, 10.0g of malt extract, 4.0g of glucose, 1000mL of 50% aged seawater, pH7.2-7.4.

[0021] (2) Seed culture: pick an activated single colony from the slant and insert it into the seed medium, and culture it at 28° C. for 2-3 days. Medium: 4.0g of yeast powder, 10.0g of malt extract, 4.0g of glucose, 1000mL of 50% aged seawater, pH7.2-7.4.

[0022] (3) Fermentation culture: 5% inoculum amount was inserted into the seed culture solution, and cultured in shake flasks at 28° C. and 180 r / min for 6 days. Medium: 10.0g corn flour, 10.0g soybean flour, 5.0g soluble starch, K 2 HPO 4 0.5g, 50% aged sea water 1000ml, pH7.2-7.4.

Embodiment 3

[0023] Example 3: Anti-root-knot nematode activity detection of bacterial strain HA12420CGMCCNo.11063

[0024] Strain HA12420CGMCCNo.11063 was fermented and cultured according to the method in Example 2, and the fermentation product fermented for 6 days was collected, centrifuged at 10,000 r / min for 10 min, and the supernatant was filtered with a 0.22 μm sterile filter membrane for future use.

[0025] (1) preparation for testing nematodes

[0026] The nematodes tested were obtained from the diseased roots of pepper collected in the wild. Take the pepper diseased root, wash the surface soil with distilled water, pick out the egg masses inside the root knot with a dissecting needle, put them into a 24-well plate filled with 0.5mL sterile water, average 10-15 egg masses per well, and let them hatch at 28°C ; Collect the second-instar larvae (J2) of the root-knot nematode that hatched, mix gently in a 2mL centrifuge tube, and prepare a nematode suspension (about 3000 / mL).

[00...

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Abstract

The invention discloses a marine actinomycete strain with meloidogyne insecticidal activity and a preparation method and application of the marine actinomycete strain. The actinomycete strain is Nocardiopsis HA12420 preserved with the preservation number CGMCC No.11063 in China General Microbiological Culture Collection Center on July 9, 2015. The preparation method of an actinomycete fermentation broth includes the steps of seed culture, ferment culture and fermentation broth purification. The actinomycete fermentation broth has distinct insecticidal activity for meloidogyne incognita, meloidogyne javanica and meloidogyne arenaria and is applicable to prevention and control of meloidogyne of vegetables, flowers, melons, crops of orchards and the like, thereby having promising development prospect in agricultural production.

Description

technical field [0001] The invention relates to a marine actinomycete with root-knot nematode killing activity and its application in preventing and controlling plant root-knot nematodes, belonging to the technical field of microorganisms. Background technique [0002] Root-knot nematode (Meloidogynes pp.) is a kind of plant parasitic nematode that is widely distributed, harmful and difficult to control. More than 90 species of root-knot nematodes have been reported, including M. incognita, M. arenaria, M. javanica and M. hapla ) belonged to the four dominant populations that occurred more commonly. Root-knot nematode disease caused by root-knot nematode damage to plant roots is a worldwide soil-borne disease, causing huge losses to agriculture, forestry, horticulture and other industries in many countries. [0003] At present, the control methods of root-knot nematode mainly include agricultural control, physical control, chemical control and biological control. The prev...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01N63/02A01P5/00C12R1/01
Inventor 黄惠琴鲍时翔朱军孙前光刘敏邹潇潇
Owner INST OF TROPICAL BIOSCI & BIOTECH CHINESE ACADEMY OF TROPICAL AGRI SCI
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