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Composite bacillus preparation for improving marine culture water environment and preparation thereof

A compound bacillus and seawater culture technology, applied in microorganism-based methods, biochemical equipment and methods, seawater treatment, etc., can solve the problems of differences in physical and chemical characteristics of bacteria, different quality and application effects, and not very obvious effects, etc. To achieve the effect of improving water quality, improving disease resistance, and enhancing the level of immune response

Inactive Publication Date: 2008-12-17
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the differences in the physical and chemical characteristics of the strains themselves, the product quality and application effects of different probiotics are also different.
The water quality improvement preparation of a single strain is often not very effective due to changes in the water environment, aquatic animal species, and addition methods

Method used

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  • Composite bacillus preparation for improving marine culture water environment and preparation thereof
  • Composite bacillus preparation for improving marine culture water environment and preparation thereof
  • Composite bacillus preparation for improving marine culture water environment and preparation thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] 1) Take beef paste powder, sodium chloride, soybean meal powder, bran and water and mix them uniformly in a weight ratio of 5:1:1:2:12 to obtain a culture medium solid;

[0015] 2) Evenly mix Bacillus licheniformis, Bacillus cereus, Bacillus natto, and Bacillus subtilis at a ratio of 1:1:2:5 to obtain a mixed bacterial solution

[0016] 3) uniformly mix the culture medium solids and the mixed bacterial liquid according to the volume ratio of 1:8, place in a fermenter, and ferment at 30° C. for 5 days to obtain a mixed fermented bacterial liquid;

[0017] 4) Evenly mix the zeolite powder and the mixed fermentation bacteria liquid according to the volume ratio of 1:4, and dry at 40°C.

[0018] The above-mentioned Bacillus licheniformis, Bacillus cereus, Bacillus natto, and Bacillus subtilis were all purchased from China Industrial Microorganism Culture Collection and Management Center. Specifically, Bacillus licheniformis CICC20085, Bacillus cereus CICC20454, Bacillus na...

Embodiment 2

[0020] 1) Take beef paste powder, sodium chloride, soybean meal powder, bran and water and mix them uniformly in a weight ratio of 5:1:1:2:12 to obtain a culture medium solid;

[0021] 2) Evenly mix Bacillus licheniformis, Bacillus cereus, Bacillus natto, and Bacillus subtilis at a ratio of 1:1:4:7 to obtain a mixed bacterial solution

[0022] 3) uniformly mix the culture medium solids and the mixed bacterial liquid according to the volume ratio of 1:10, place in a fermenter, and ferment at 31°C for 6 days to obtain a mixed fermented bacterial liquid;

[0023] 4) Evenly mix the bentonite and the mixed fermentation bacteria liquid according to the volume ratio of 1:5, and dry at 45°C.

Embodiment 3

[0025] 1) Take beef paste powder, sodium chloride, soybean meal powder, bran and water and mix them uniformly in a weight ratio of 5:1:1:2:12 to obtain a culture medium solid;

[0026] 2) uniformly mixing Bacillus licheniformis, Bacillus cereus, Bacillus natto, and Bacillus subtilis at a ratio of 1:1:6:9, and fermenting to obtain a composite Bacillus preparation for improving the environment of the breeding water;

[0027] 3) uniformly mix the culture medium solids and the mixed bacterial liquid according to the volume ratio of 1:12, place in a fermenter, and ferment for 7 days at 33° C. to obtain a mixed fermented bacterial liquid;

[0028] 4) Evenly mix the medical stone powder and the mixed fermentation bacteria liquid according to the volume ratio of 1:6, and dry at 46°C.

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Abstract

The invention discloses a composite bacillus preparation applied to improving the aquatic environment of mariculture, and a making method thereof. The preparation is made by mixing Bacillus subtilis, Bacillus licheniformis, Bacillus cereus and Bafillus natto according to certain ratio, and under room temperature, the active microorganism is in resting state and the preparation is stable and easy to be preserved. After the composite bacillus preparation is put into mariculture water, the dormant bacteria can be back to normal quickly, degrade residual bait, dejection, fertilizer, biological residual bodies and other organic contaminant fast, reduce the content of ammonia nitrogen, nitrite and COD in the culture water and reproduce to be predominant bacteria in the water quickly so as to further restrain the putrefying bacteria from reproduction, improve the environment of the culture water and purify the water. The preparation of the invention is safe, contains no pollution nuisance and residual, can obviously improve the environment of the culture water, and meanwhile, the immunity of aquatic livestock can be improved.

Description

technical field [0001] The invention relates to the field of aquaculture water quality improvement, in particular to a compound bacillus preparation for improving the water environment of seawater aquaculture and a preparation method thereof. Background technique [0002] In recent decades, high-density, industrialized aquaculture has developed vigorously in my country, which largely meets people's demand for aquatic products and solves the contradiction of relative shortage of natural fishery resources. However, breeding residues, fish and shrimp feces, biological residues, fertilizers, etc. accumulate at the bottom of the pond. These pollutants are the source of deterioration of pond water quality and the hotbed of various germs and viruses. The deterioration of the ecological environment. Many chemical drugs are widely used as fish medicines, feed additives, water quality improvers, etc., resulting in more and more serious problems such as pathogen resistance, animal flo...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/125C12R1/10C12R1/085C12R1/07C02F103/08
Inventor 吴天星潘晓东
Owner ZHEJIANG UNIV
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