Fermented liquid feed for Pig and poultry as well as preparation method and application thereof
A liquid feed, pig and poultry technology, applied in the biological field, can solve the problems of poor reproduction repeatability, large equipment investment, and low product quality, and achieve the effects of increasing microbial species, improving product performance, and shortening reaction time.
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Embodiment 1
[0025] 1) First-level slant cultivation:
[0026] Lactobacillus and Lactobacillus fermentum were respectively cultured at 28 for 36 hours; the culture medium of the above two bacteria was a mixture prepared in the following ratio: peptone 0.5wt%, beef extract 1wt%, triammonium citrate 0.1wt%, acetic acid Sodium (3H 2 O) 0.7wt%, yeast extract powder 0.2wt%; Glucose 1wt%; Dipotassium hydrogen phosphate 0.3wt%; Tween 800.1wt%; Agar 1.3wt%;
[0027] Bacillus subtilis and Bacillus natto were cultured at 30°C for 32 hours respectively; the medium of the above two bacteria was a mixture prepared in the following proportions: 0.3wt% peptone, 0.4wt% glucose, 1.5wt% beef extract, diphosphate Potassium hydrogen 0.4wt%, agar 3wt%;
[0028] Cultivate Saccharomyces cerevisiae at 26°C for 30 hours; the culture medium of Saccharomyces cerevisiae is a mixture prepared according to the following proportions: soybean peptone 0.2wt%, glucose 0.3wt%, yeast extract 0.5wt%, malt extract 1wt%, agar...
Embodiment 2
[0037] 1) First-level slant cultivation:
[0038] Lactobacillus and Lactobacillus fermentum were respectively cultured at 38 for 18 hours; the culture medium of the above two bacteria was a mixture prepared in the following ratio: peptone 1wt%, beef extract 0.5%, triammonium citrate 0.3wt%, sodium acetate (3H2O) 0.7wt%, yeast extract powder 0.7wt%; Glucose 2wt%; Dipotassium hydrogen phosphate 0.1wt%; Tween 800.05wt%; Agar 1.6wt%;
[0039] Cultivate Bacillus subtilis and Bacillus natto at 37°C for 18 hours respectively; the culture medium of the above two bacteria is a mixture prepared in the following proportions: peptone 1wt%, glucose 2wt%, beef extract 0.3wt%, potassium dihydrogen phosphate 0.4wt%, agar 4wt%;
[0040] Cultivate Saccharomyces cerevisiae at 30°C for 24 hours; the culture medium of Saccharomyces cerevisiae is a mixture prepared according to the following proportions: soybean peptone 0.7wt%, glucose 1.0wt%, yeast extract 0.1wt%, malt extract 0.2wt%, agar powder...
Embodiment 3
[0049] 1) First-level slant cultivation:
[0050] Lactobacillus and Lactobacillus fermentum were cultured at 37°C for 24 hours respectively; the culture medium of the above two bacteria was a mixture prepared in the following proportions: peptone 0.8wt%, beef extract 0.5wt%, triammonium citrate 0.2wt%, Sodium acetate (3H 2 O) 0.5wt%, yeast extract powder 0.5wt%; Glucose 1.5wt%; Dipotassium hydrogen phosphate 0.2wt%; Tween 800.08wt%; Agar 1.5wt%;
[0051] Bacillus subtilis and Bacillus natto were respectively cultivated at 37°C for 18 hours; the medium of the above two bacteria was a mixture prepared in the following proportions: 0.5wt% peptone, 1.5wt% glucose, 1.0wt% beef extract, diphosphate Potassium hydrogen 0.5wt%, agar 3wt%;
[0052] Cultivate Saccharomyces cerevisiae at 30°C for 24 hours; the culture medium of Saccharomyces cerevisiae is a mixture prepared according to the following proportions: soybean peptone 0.5wt%, glucose 0.8wt%, yeast extract 0.3wt%, malt extract...
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