Fermented liquid feed for Pig and poultry as well as preparation method and application thereof

A liquid feed, pig and poultry technology, applied in the biological field, can solve the problems of poor reproduction repeatability, large equipment investment, and low product quality, and achieve the effects of increasing microbial species, improving product performance, and shortening reaction time.

Active Publication Date: 2009-10-21
BEIJING DABEINONG TECH GRP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The fermentation methods of fermented feed include solid fermentation method and liquid fermentation method, but the fermented products generally have defects such as low quality, serious bacterial contamination, perishable, unsatisfactory use effect, etc. The fermentation method itself also has its own shortcomings
For example: Patent CN1399905A discloses a method for preparing circulating feed for pigs. In this method, trace elements, amino acids, cakes, etc. are mixed with solid microbial strains in a fermentation cellar of cement masonry structure, air is discharged, and then sealed and fermented for 10 The disadvantage is that the fermentation environment is uncontrollable, the product quality cannot be guaranteed, and the microorganisms cannot be well attached to the surface of the substrate in solid-phase fermentation, the reproduction repeatability is poor, and the fermentation cycle is long, and the fermentation effect is far lower than that of liquid fermentation.
Patent CN1973661A disclo...

Method used

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  • Fermented liquid feed for Pig and poultry as well as preparation method and application thereof
  • Fermented liquid feed for Pig and poultry as well as preparation method and application thereof
  • Fermented liquid feed for Pig and poultry as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] 1) First-level slant cultivation:

[0026] Lactobacillus and Lactobacillus fermentum were respectively cultured at 28 for 36 hours; the culture medium of the above two bacteria was a mixture prepared in the following ratio: peptone 0.5wt%, beef extract 1wt%, triammonium citrate 0.1wt%, acetic acid Sodium (3H 2 O) 0.7wt%, yeast extract powder 0.2wt%; Glucose 1wt%; Dipotassium hydrogen phosphate 0.3wt%; Tween 800.1wt%; Agar 1.3wt%;

[0027] Bacillus subtilis and Bacillus natto were cultured at 30°C for 32 hours respectively; the medium of the above two bacteria was a mixture prepared in the following proportions: 0.3wt% peptone, 0.4wt% glucose, 1.5wt% beef extract, diphosphate Potassium hydrogen 0.4wt%, agar 3wt%;

[0028] Cultivate Saccharomyces cerevisiae at 26°C for 30 hours; the culture medium of Saccharomyces cerevisiae is a mixture prepared according to the following proportions: soybean peptone 0.2wt%, glucose 0.3wt%, yeast extract 0.5wt%, malt extract 1wt%, agar...

Embodiment 2

[0037] 1) First-level slant cultivation:

[0038] Lactobacillus and Lactobacillus fermentum were respectively cultured at 38 for 18 hours; the culture medium of the above two bacteria was a mixture prepared in the following ratio: peptone 1wt%, beef extract 0.5%, triammonium citrate 0.3wt%, sodium acetate (3H2O) 0.7wt%, yeast extract powder 0.7wt%; Glucose 2wt%; Dipotassium hydrogen phosphate 0.1wt%; Tween 800.05wt%; Agar 1.6wt%;

[0039] Cultivate Bacillus subtilis and Bacillus natto at 37°C for 18 hours respectively; the culture medium of the above two bacteria is a mixture prepared in the following proportions: peptone 1wt%, glucose 2wt%, beef extract 0.3wt%, potassium dihydrogen phosphate 0.4wt%, agar 4wt%;

[0040] Cultivate Saccharomyces cerevisiae at 30°C for 24 hours; the culture medium of Saccharomyces cerevisiae is a mixture prepared according to the following proportions: soybean peptone 0.7wt%, glucose 1.0wt%, yeast extract 0.1wt%, malt extract 0.2wt%, agar powder...

Embodiment 3

[0049] 1) First-level slant cultivation:

[0050] Lactobacillus and Lactobacillus fermentum were cultured at 37°C for 24 hours respectively; the culture medium of the above two bacteria was a mixture prepared in the following proportions: peptone 0.8wt%, beef extract 0.5wt%, triammonium citrate 0.2wt%, Sodium acetate (3H 2 O) 0.5wt%, yeast extract powder 0.5wt%; Glucose 1.5wt%; Dipotassium hydrogen phosphate 0.2wt%; Tween 800.08wt%; Agar 1.5wt%;

[0051] Bacillus subtilis and Bacillus natto were respectively cultivated at 37°C for 18 hours; the medium of the above two bacteria was a mixture prepared in the following proportions: 0.5wt% peptone, 1.5wt% glucose, 1.0wt% beef extract, diphosphate Potassium hydrogen 0.5wt%, agar 3wt%;

[0052] Cultivate Saccharomyces cerevisiae at 30°C for 24 hours; the culture medium of Saccharomyces cerevisiae is a mixture prepared according to the following proportions: soybean peptone 0.5wt%, glucose 0.8wt%, yeast extract 0.3wt%, malt extract...

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Abstract

The invention relates to a fermented liquid feed for pig and poultry as well as a preparation method and an application thereof, belonging to the field of biotechnology, in particular to a liquid feed produced by the fermentation of a plurality of beneficial microbes and Chinese medicinal herbs as well as a preparation method and an application thereof. The preparation method has simple process and comprises the following steps: after primary slant culture and secondary solution culture, lactobacillus, fermentation lactobacillus, bacillus subtilis, natto bacillus and saccharomyces cerevisiae are put into a solid fermenting tank with a fermentation medium containing astragalus root, Na2HPO4, nitrogen source and carbon according to weight proportion. The fermented liquid feed adopting the method has high quality, thicker mellow flavor and short fermenting time, contains protein, polypeptide, small peptide, amino acid, astragalus polyose and a plurality of beneficial microbes and is applicable to preparing various perfect compound feeds for pig and poultry.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a fermented liquid feed suitable for pigs and poultry and its preparation method and application, in particular to a liquid feed fermented by various beneficial microorganisms and its preparation method and application. Background technique [0002] The disadvantages of the abuse of antibiotics in the aquaculture industry have become prominent, and it has become a trend to carry out green and healthy aquaculture, and to produce and apply green and safe feed. Fermented feed eliminates anti-nutritional factors through fermentation, increases feed intake and digestion and absorption of nutrients, improves the overall health of animals, reduces the incidence of respiratory and digestive tract diseases, reduces ammonia and odor emissions, and improves Ecological environment, etc., have been favored in recent years. The fermentation methods of fermented feed include solid fermentation metho...

Claims

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Application Information

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IPC IPC(8): A23K1/16A23K1/18A23K10/12A23K50/30A23K50/75
Inventor 赵雁青张玳华姚琨贾秋英白玉卿孙占敏刘宇赵军
Owner BEIJING DABEINONG TECH GRP CO LTD
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