Fermented feed additive, preparation method and application
A technology of fermented feed and additives, applied in the fields of application, animal feed, animal feed, etc., can solve the problems of difficult fermented feed technology, achieve the effects of reducing pollution and environmental protection treatment costs, improving conversion utilization, and reducing the use of antibiotics
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Embodiment 1
[0032] Under sterile conditions, scrape a loop of the strains of Lactobacillus acidophilus, Lactobacillus plantarum and Lactobacillus casei with an inoculation needle, and inoculate them into a petri dish containing lactic acid bacteria activation medium, and the lactic acid bacteria activation The medium is peptone 10g / l, meat extract 10g / l, yeast extract 5g / l, K 2 HPO 4 2g / l, triammonium citrate 2g / l, sodium acetate 5g / l, glucose 20g / l, Tween80 1mL / l, MgSO 4 ·7H 2 O 0.58g / l, MnSO 4 4H 2 O 0.25g / l, agar 20g / l and CaCO 3 After mixing 10g / l, the pH value is adjusted to 6.2, and it is obtained by sterilizing at 121°C for 15 minutes; scrape a ring of the strain of Saccharomyces cerevisiae with an inoculation needle, and inoculate it into a petri dish containing yeast activation medium , the saccharomyces activating medium is obtained after potato 200g / l is prepared into potato juice, glucose 20g / l and agar 20g / l and mixed; One ring, inoculated into a petri dish filled with ...
Embodiment 2
[0035] Under sterile conditions, scrape a loop of the strains of Lactobacillus acidophilus, Lactobacillus plantarum and Lactobacillus casei with an inoculation needle, and inoculate them into a petri dish containing lactic acid bacteria activation medium, and the lactic acid bacteria activation The medium is peptone 12g / l, meat extract 12g / l, yeast extract 8g / l, K 2 HPO 4 4g / l, triammonium citrate 4g / l, sodium acetate 8g / l, glucose 25g / l, Tween80 2mL / l, MgSO 4 ·7H 2 O 1g / l, MnSO 4 4H 2 O 0.5g / l, agar 25g / l and CaCO 3 After mixing 12g / l, the pH value is adjusted to 6.4, and it is obtained by sterilizing at 121°C for 20 minutes; scrape a ring of the strain of Saccharomyces cerevisiae with an inoculation needle, and inoculate it into a petri dish containing yeast activation medium , the saccharomyces activation medium is obtained after potato juice, glucose 25g / l and agar 25g / l are mixed by preparing potato 220g / l; One ring, inoculated into a petri dish filled with bacillus...
Embodiment 3
[0038] Under sterile conditions, scrape a loop of the strains of Lactobacillus acidophilus, Lactobacillus plantarum and Lactobacillus casei with an inoculation needle, and inoculate them into a petri dish containing lactic acid bacteria activation medium, and the lactic acid bacteria activation The medium is peptone 11g / l, meat extract 11g / l, yeast extract 6g / l, K 2 HPO 4 3g / l, triammonium citrate 3g / l, sodium acetate 6g / l, glucose 22g / l, Tween80 1.5mL / l, MgSO 4 ·7H 2 O 0.7g / l, MnSO4 4H 2 O 0.3g / l, agar 22g / l and CaCO 3 After mixing 11g / l, the pH value is adjusted to 6.3, and it is obtained by sterilizing at 121°C for 15 minutes; scrape a ring of the strain of Saccharomyces cerevisiae with an inoculation needle, and inoculate it into a petri dish containing yeast activation medium , the saccharomyces activation medium is obtained after potato juice, glucose 22g / l and agar 22g / l are mixed by preparing potato 210g / l; One ring, inoculated into a petri dish filled with bacill...
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