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Method for improving forage nutritional value of cottonseed meal through microbial fermentation

A technology of microbial fermentation and nutritional value, applied in animal feed, animal feed, application, etc., can solve the problems of lack of application effect of improving the nutritional value of cotton meal, adverse effects on growth, development and reproduction, and limiting the wide application of cotton meal, etc. The effect of promoting degradation, strong ability, and improving efficacy

Inactive Publication Date: 2014-12-10
QINGDAO JIARUI BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, cottonseed meal contains the toxic substance gossypol, which will have obvious adverse effects on animal growth, development and reproduction; it also contains anti-nutritional factors such as cellulose, oligosaccharides (raffinose, stachyose), and phytic acid; crude The protein content is high, but the protein quality is poor, and the content of essential amino acids such as methionine is low and unbalanced. The above factors limit the wide application of cotton meal in the breeding industry
For a long time, in order to improve the application of cottonseed meal in feed, researchers have carried out research on the screening of gossypol efficient detoxification strains and their detoxification effects, but there is no significant application effect on the improvement of cottonseed meal's nutritional value

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] The method for improving the nutritional value of cottonseed meal by microbial fermentation, the specific preparation steps are as follows:

[0024] (1) Pretreatment: select cotton meal without mildew, crush it through a 40-mesh sieve, and adjust the moisture content of cotton meal to about 12-18%;

[0025] (2) Enzymolysis: adjust the pH of cotton meal to 4.0-5.0, add cellulase and xylanase, the enzyme amount (mass ratio) is 3%, 1%, mix well, enzymolysis 16h, enzymolysis temperature control At 40-50°C;

[0026] (3) Preparation of solid medium: add 5% (relative to the mass ratio of cotton meal, the same below) molasses, 2% corn steep liquor, 2% urea, 1% ammonium sulfate, 0.2% diphosphate to the enzymatically hydrolyzed cotton meal Potassium hydrogen, adjust the water content of the medium to 30-40%, and adjust the initial pH to about 7.0;

[0027] (4) Aspergillus activation: take fresh bran, sieve fine powder with a 60-mesh sieve, add water according to the ratio of br...

Embodiment 2

[0036] (1) Pretreatment: select cotton meal without mildew, crush it through a 40-mesh sieve, and adjust the moisture content of cotton meal to about 12-18%;

[0037] (2) Enzymolysis: adjust the pH of cotton meal to 4.0-5.0, add cellulase and xylanase, the amount of enzyme added (mass ratio) is 2%, 2%, mix well, enzymolysis 16-24h, enzymolysis The temperature is controlled at 40-50°C;

[0038] (3) Preparation of solid medium: add 3% (relative to the mass ratio of cotton meal, the same below) molasses, 3% corn steep liquor, 1.5% urea, 1.5% ammonium sulfate, 0.1% diphosphate to the enzymatically hydrolyzed cotton meal Potassium hydrogen, adjust the water content of the medium to 30-40%, and adjust the initial pH to about 7.0;

[0039] (4) Aspergillus activation: Take fresh bran, sieve fine powder with a 60-mesh sieve, add water according to the ratio of bran:water=1:1.3, mix well until there is no dry powder and no agglomeration. After mixing well, divide into 2000mL conical f...

Embodiment 3

[0048] (1) Pretreatment: select cotton meal without mildew, crush it through a 40-mesh sieve, and adjust the moisture content of cotton meal to about 12-18%;

[0049] (2) Enzymolysis: Adjust the pH of cottonseed meal to 4.0-5.0, add cellulase and xylanase, the enzyme amount (mass ratio) is 2.5%, 1.5%, mix well, enzymolysis 16-24h, enzymolysis The temperature is controlled at 40-50°C;

[0050] (3) Preparation of solid medium: add 4% (relative to the mass ratio of cotton meal, the same below) molasses, 2.5% corn steep liquor, 1.8% urea, 1.2% ammonium sulfate, 0.15% diphosphate to the enzymatically hydrolyzed cotton meal Potassium hydrogen, adjust the water content of the medium to 30-40%, and adjust the initial pH to about 7.0;

[0051] (4) Aspergillus activation: take fresh bran, sieve fine powder with a 60-mesh sieve, add water according to the ratio of bran: water = 1: 1.2, mix well until there is no dry powder and no agglomeration. After mixing well, divide into 2000mL con...

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Abstract

The invention utilizes a microbial fermentation technology to improve the forage nutritional value of the cottonseed meal, so as to improve the forage quality of the cottonseed meal, increase the addition proportion in the forage and realize the promotion of the protein nutritional value and the feeding value of the cottonseed meal, aiming at the disadvantages that cottonseed meal is rich in antinutritional factors of free gossypol, cellulose, oligosaccharide, phytic acid and the like, the protein quality is relatively poor and the content of essential amino acids of methionine and the like is low and unbalanced, so that the large-scale forage of cottonseed meal is restricted. The method for improving forage nutritional value of cottonseed meal through the microbial fermentation adopts the technology of 'composite enzymatic hydrolysis and multi-strain three-step solid-state fermentation', so as to prepare the fermented cottonseed meal product that is high in the free gossypol, cellulose degradation degree and the protein content; the cottonseed meal product is rich in nutritional ingredients of probiotics, vitamins, enzyme, amino acid, short peptide, high-grade protein and the like; the method for improving the forage nutritional value of cottonseed meal through the microbial fermentation has the advantages of prompting growth and development, improving the utilization ratio of the forage, enhancing immunization, improving intestinal micro ecology, preventing diseases and the like. The method for improving forage nutritional value of cottonseed meal through the microbial fermentation adopts the solid-state fermentation; the manufacturing technology is simple; the energy consumption is low; zero environmental pollution is generated; and the investment is small. Therefore, mass production is easy.

Description

technical field [0001] The invention relates to a method for improving the nutritional value of cottonseed meal by microbial fermentation, and belongs to the field of feed processing. Background technique [0002] Protein raw materials are the foundation of the development of feed industry. The shortage and high price of protein raw materials in my country have become the bottleneck restricting the development of animal husbandry. At present, with the improvement of people's living standards and the emphasis on the quality of life, the demand for meat, eggs, milk, and aquatic products is increasing day by day. The production of grain and feed is facing severe challenges, and feed protein resources will become increasingly scarce. It is imminent to expand feed resources and develop unconventional feed. The important strategy to alleviate the contradiction is to improve the utilization rate of the existing protein source, tap the available nutritional components of the feed a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/14
Inventor 董书阁侯文燕代书强张术聪肖龙虻陈希凯
Owner QINGDAO JIARUI BIOLOGICAL TECH
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