Method for preparing high-purity rice protein and high-purity rice peptide

Abstract

The invention relates to a method for preparing high-purity rice protein and high-purity rice peptide. Rice or broken rice is used as a raw material in the method; based on crude rice protein prepared by an alkali extraction and acid precipitation method, non-protein substances in the crude rice protein are removed by the impurity removing effect of an efficient composite enzyme consisting of amylase, cellulase and lipase, and then the high-purity rice protein with protein content of more than 90 percent is prepared; and based on the high-purity rice protein, the hydrolysis degree of the rice protein is controlled under the action of the composite enzyme by an alternated enzymolysis ultra-filtration coupling technology, and the high-purity rice peptide with protein content of over 90 percent, polypeptide content of more than 70 percent and 200 to 1,200Da oligopeptide content of over 50 percent is prepared by separation.

Description

technical field [0001] The invention belongs to the technical field of rice deep processing, in particular to a method for preparing high-purity rice protein and high-purity rice peptide. Background technique [0002] As a high-nutrition, hypoallergenic grain protein, rice protein is very suitable as a protein supplement for infants, the elderly, and patients, so it has become the darling of the world's protein market. Rice protein products can be divided into feed grade and food grade according to protein purity. The protein content of feed grade is 40% to 70%, and the protein content of food grade is above 80%. The difference in protein purity leads to a very large price gap between the two. The price of food-grade rice protein products can be as high as 30,000 to 50,000 yuan per ton. At present, the rice protein products produced by most domestic enterprises are feed-grade rice protein products. The process adopts simple alkali extraction and acid precipitation process, a...

AI Technical Summary

Problems solved by technology

Currently widely used intermittent enzymatic hydrolysis process, before the completion of enzymatic hydrolysis, the polypeptide product and protease are always in the same reaction system, the degree of polypeptide hydrolysis is difficult to control, if the degree of hydrolysis is too large, too many free amino acids will be produced, but If the degree of hydrolysis is small, although the amount of free amino acids will decrease, the amount of polypeptides with larger molecular weight will also increase, so under this process, there is a contradiction between reducing the content of free amino acids and increasing the content of oligopeptides