Chromium-rich yeast culture and fermentation process thereof

Abstract

The invention relates to a microorganism and a microbial fermentation process thereof, in particular to a chromium-rich yeast culture and a fermentation process thereof. The process is characterized by selecting and screening Candida tropicalis and saccharomyces cerevisiae tolerant of high chromium and carrying out production through a liquid-solid two-phase fermentation process. The process flow comprises that: high-chromium-tolerant (trivalent) strains are screened; the strains are progressively expanded and cultured; liquid fermentation is performed to transform inorganic chromium, wherein the content of the inorganic chromium in a culture medium is 0.05 to 0.4 percent, preferably 0.1 to 0.2 percent, and the time of fermentation and transformation is 12 to 48 hours, preferably 18 to 26hours; after the liquid fermentation is over, culture solution and a solid culture medium are mixed according to the weight ratio of 1:3-1.5:1, preferably 1:1, and are subjected to solid aerobic fermentation for 16 to 36 hours, preferably 16 to 24 hours; after the solid aerobic fermentation is over, solid anaerobic fermentation is performed to break the wall of yeast at a temperature kept between40 and 60 DEG C for 6 to 24 hours, preferably at 50 DEG C for 6 to 10 hours; and a finished product after drying contains organic chromium accounting for 0.05 to 0.3 percent. The proportion of transforming the inorganic chromium into the organic chromium by utilizing a technique of the invention is more than 85 percent, and no hexavalent chromium is detected. The chromium-rich yeast culture produced in the invention has the characteristics of simple process, low production cost, good product quality and the like, has the double function of organic chromium and probiotic feed, and can be used for feed of livestock, poultry and aquatic products.

Description

Technical field [0001] The invention relates to a microorganism and its fermentation process, in particular to a chromium-rich yeast culture and its fermentation process. Background technique [0002] Chromium is an active component of glucose tolerance factor (Glucose Tolerance Factor, GTF), which mainly exerts biological effects by enhancing the action of insulin. Chromium (trivalent) is an essential trace element for animals. The content of chromium in ordinary feed ingredients is low; the utilization rate of additional inorganic chromium is less than 1%; the kidneys cannot reabsorb it, and the loss is rapid, especially under stress conditions. Therefore, the use of organic chromium is advocated in feed, and the absorption and utilization rate of organic chromium is between 10-40%. The organic chromium currently used mainly includes chromium picolinate, chromium formate, amino acid chromium and yeast chromium. Among them, yeast chromium has the best effect on pig feeding. The ...

AI Technical Summary

Problems solved by technology

This type of process has the following disadvantages: ① The probiotic ingredients produced by yeast fermentation are limited; ② The investment amount is large; ③ The