Preparation method of composite bacteria fermentation bed
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
A technology of composite bacterial agent and fermentation bed, which is applied in the field of preparation of composite bacterial agent fermentation bed, and can solve the problems of bulky volume, environmental pollution, and low density.
Inactive Publication Date: 2009-02-04
NORTHEAST AGRICULTURAL UNIVERSITY
View PDF0 Cites 17 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
[0006] At present, the remaining amount of straw in our country is increasing. Since these wastes are small in density, bulky in volume, and piled up in large quantities, destruction and dis
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Image
Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
Click on the blue label to locate the original text in one second.
Reading with bidirectional positioning of images and text.
Smart Image
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0043] 1. Selection of fermentation strains and preparation of medium
[0044] Bacteria A——Candida tropicalis (ACCC2004), the medium is 10°Bx or 12°Bx fresh wort 1000mL, pH5.5-6.0.
[0045] Bacteria——Lactobacillus plantarum (AS1.557), the medium is glucose 20g, sodium acetate 5g, peptone 10g, sodium citrate 5g, beef extract 10g, dipotassium hydrogen phosphate 2g, yeast powder 5gMgSO 4 ·7H 2 O 0.58g, Tween-80 1ml, MnSO 4 4H 2 O0.25g, distilled water 1000ml, adjust the pH value to 6.2.
[0046] Bacteria C—Streptococcus thermophilus (IFFI06038), the medium is polypeptone 5g, plant peptone 5g, beef extract 5g, yeast extract 2.5g, disodium phosphate glycerol 19g, ascorbic acid 0.5g, MgSO 4 ·7H 2 O 0.25g, lactose 5g, pH7.1, distilled water 1000mL.
[0047] Bacteria D——Bacillus subtilis (AS 1.108), the medium is peptone 10g, beef extract 3g, NaCL 5g, distilled water 1000ml, pH7.4.
[0048] F bacterium——Bacillus subtilis (ACCC11062), the culture medium is peptone 10g, beef extr...
Embodiment 2
[0064] 1. Selection of fermentation strains and preparation of medium
[0065] Bacteria A——Candida tropicalis (ACCC2004), the medium is 10°Bx or 12°Bx fresh wort 1000mL, pH5.5-6.0.
[0066] Bacteria——Lactobacillus plantarum (AS1.557), the medium is glucose 20g, sodium acetate 5g, peptone 10g, sodium citrate 5g, beef extract 10g, dipotassium hydrogen phosphate 2g, yeast powder 5gMgSO 4 ·7H 2 O 0.58g, Tween-80 1ml, MnSO 4 4H 2 O0.25g, distilled water 1000ml, adjust the pH value to 6.2.
[0067] Bacteria C—Streptococcus thermophilus (IFFI06038), the medium is polypeptone 5g, plant peptone 5g, beef extract 5g, yeast extract 2.5g, disodium phosphate glycerol 19g, ascorbic acid 0.5g, MgSO 4 ·7H 2 O 0.25g, lactose 5g, pH7.1, distilled water 1000mL.
[0068] Bacteria D——Bacillus subtilis (AS 1.108), the medium is peptone 10g, beef extract 3g, NaCL 5g, distilled water 1000ml, pH7.4.
[0069] Bacteria E—Bacillus subtilis (ACCC11062), the culture medium is peptone 10g, beef extrac...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
PUM
Login to view more
Abstract
The invention provides a preparation method of a compound bacteria fermentation bed. Firstly, five production ferments of candida tropicalis, lactobacillus plantarum, streptococcus thermophilus, Bacillu subtilis AS 1.108 and Bacillu subtilis ACCC 11062 are prepared, the living bacterium of each production ferment is not less than 10<9 >/ml, afterward, the five production ferments of lactobacillus are freeze-dried. According to the weight proportion of the freeze-dried bacteria powder, 2-3 parts of candida tropicalis powder, 2-5 parts of lactobacillus plantarum powder, 2-4 parts of streptococcus thermophilus powder, 1-3 parts of Bacillu subtilis AS 1.108 powder and 3-5 parts of Bacillu subtilis ACCC 11062 powder are taken out, well mixed and vacuum-packaged thereafter, thereby obtaining the compound bacteria. After that, the culture medium of the fermentation bed is made from 1 percent glucose, 2 percent rice hull (uncrushed), 15 percent water and 82 percent kibbled straw. The compound bacteria is added into the culture medium with the weight proportion of adding 10 grams compound bacteria into every 100 kilograms culture medium after being dissolved by a little warm water to be well mixed, and then tiled and compacted with a thickness less than 15cm. The compound bacteria fermentation bed can be completely fermented in 5 days under natural conditions. The use of the production of the invention can reduce the smell of the pigsty and the proliferation possibility of flies and mosquitoes, thus ensuring that the pigs can grow healthily and providing people with safe pork while protecting the environment.
Description
(1) Technical field [0001] The invention relates to a method for preparing a composite bacterial agent fermentation bed for raising pigs, and belongs to the field of feed additives. (2) Background technology [0002] The annual growth rate of breeding scale and output value in our country exceeds 10%, but at the same time, the environmental pollution brought by the breeding industry is becoming more and more serious. A survey conducted by the State Environmental Protection Administration in 23 provinces and cities across the country found that 90% of the large-scale livestock and poultry farms in the country have not passed the environmental impact assessment, and 60% of the farms lack the necessary pollution prevention and control measures. Untreated livestock and poultry manure accumulates arbitrarily, and sewage manure is discharged randomly, which brings a series of environmental problems such as occupying and polluting farmland and water bodies, producing stench and cau...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.