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Heavy metal contaminated soil microbe repairing agent and application thereof

A technology for microbial remediation and contaminated soil, which is applied in the application field of microbial remediation agents in soil remediation. It can solve the problems of affecting the remediation effect, affecting microbial activity, and insignificant treatment effect, and achieves engineering convenience, strong adsorption and passivation, Good effect of promoting application value

Inactive Publication Date: 2015-04-22
SOOCHOW KH BIO SCI & TECH CO LTD KHB
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The advantages of microbial remediation measures are simple implementation, less investment and less damage to the environment. The disadvantage is that the treatment effect has not been significant so far, because microbial remediation is easily affected by various environmental factors, such as temperature, oxygen, and moisture. activity, thereby affecting the repair effect

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A heavy metal contaminated soil microbial remediation agent, which comprises the following components:

[0023] 10 parts of Bacillus subtilis,

[0024] Candida tropicalis 5 parts,

[0025] Nutrients 10 parts,

[0026] 50 parts of bentonite.

[0027] The Bacillus subtilis is a strain with the preservation number CMCC63525;

[0028] The Candida tropicalis is at least the strain with the preservation number ACCC20004;

[0029] The effective colony number of described bacillus subtilis is 20 * 10 8 cfu / g, the effective colony number of described Candida tropicalis is 30×10 8 cfu / g.

[0030] The nutrients are nitrogen, phosphorus, potassium, zinc and iron, and the weight ratio of nitrogen, phosphorus, potassium, zinc and iron is N:P 2 o 5 :K 2 O: ZnO: Fe 2 o 3 =1.1:1.8:1.5:3:1.

Embodiment 2

[0032] A heavy metal contaminated soil microbial remediation agent, which comprises the following components:

[0033] 30 parts of Bacillus subtilis,

[0034] Candida tropicalis 10 parts,

[0035] Nutrients 30 parts,

[0036] 200 parts of meerschaum.

[0037] The Bacillus subtilis is a strain with the preservation number CMCC63521;

[0038] The Candida tropicalis is the strain in the preservation number ACCC20148;

[0039] The effective colony number of described bacillus subtilis is 80 * 10 8 cfu / g, the effective colony number of described Candida tropicalis is 50×10 8 cfu / g.

[0040]The nutrients are nitrogen, phosphorus, potassium, zinc and iron, and the weight ratio of nitrogen, phosphorus, potassium, zinc and iron is N:P 2 o 5 :K 2 O: ZnO: Fe 2 o 3 =1.1:1.8:1.5:3:1.

Embodiment 3

[0042] A heavy metal contaminated soil microbial remediation agent, which comprises the following components:

[0043] 20 parts of Bacillus subtilis,

[0044] Candida tropicalis 80 parts,

[0045] Nutrients 20 parts,

[0046] Carrier 100 parts.

[0047] The carrier is a mixture of bentonite and sepiolite with a weight ratio of 0.5:1.

[0048] The Bacillus subtilis is a strain with the preservation number CMCC63522;

[0049] The Candida tropicalis is a strain with the preservation number ACCC20006;

[0050] The effective colony number of described bacillus subtilis is 50 * 10 8 cfu / g, the effective colony number of described Candida tropicalis is 40×10 8 cfu / g.

[0051] The nutrients are nitrogen, phosphorus, potassium, zinc and iron, and the weight ratio of nitrogen, phosphorus, potassium, zinc and iron is N:P 2 o 5 :K 2 O: ZnO: Fe 2 o 3 =1.1:1.8:1.5:3:1.

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Abstract

The invention provides a heavy metal contaminated soil microbe repairing agent which comprises the following components: brevibacillus borstelensis, candida tropicalis, nutrients and a carrier. The heavy metal contaminated soil microbe repairing agent is used in the heavy metal contaminated soil repairing microbe, the microbe used in the heavy metal contaminated soil microbe repairing agent can rapidly colonize and grow in soil and form a strong colony, can directly adsorb heavy metal ions into the microbial cell walls or cells for curing and passivation, heavy metal mobility is decreased, and crop heavy metal ion absorption can be reduced. The technology is low in repair cost and convenient in construction, provides a new method for soil microbe repairing, and has good application value.

Description

technical field [0001] The invention relates to a microbial remediation agent for remediating soil polluted by heavy metals, especially cadmium, and the application of the microbial remediation agent in soil remediation. Background technique [0002] Heavy metal pollution refers to the pollution caused by metals or their compounds with a specific gravity greater than 5 or 4 (mainly including Cu, Zn, Cd, Pb, Hg, Cr, As, Ni, Co) in the environment. Heavy metal elements can be divided into two categories according to their biochemical properties: one is an essential element that can maintain the normal physiological activities of living organisms within a certain concentration range, but if its concentration exceeds a certain range, it will cause body poisoning, such as copper and zinc The other category is non-essential elements for normal physiological activities of organisms, that is, harmful elements, such as cadmium, mercury, lead, etc. Cadmium (cd) is a heavy metal eleme...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/16B09C1/10C12R1/125C12R1/74
CPCB09C1/10C12N1/16C12N1/20
Inventor 金京勋
Owner SOOCHOW KH BIO SCI & TECH CO LTD KHB
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