Preparation method of amino acid leaf fertilizer

A foliar fertilizer and amino acid technology, which is applied in the directions of organic fertilizers, fertilization devices, fertilizers made from biological waste, etc., can solve the problems of high production costs, unfavorable popularization and use of amino acid foliar fertilizers, and high prices of amino acid foliar fertilizers. The effect of reducing pollution, realizing value-added and resource utilization, and low-cost process

Active Publication Date: 2014-05-21
江苏联海生物科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The preparation method of the amino acid foliar fertilizer in the prior art is mainly to use plant amino acid and various minerals and other nutritional components to prepare. This method has high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] A method for producing amino acid foliage fertilizer with alcohol fermentation waste water as main raw material, comprising steps as follows

[0045] (1) Preparation of seed culture medium

[0046] A. Preparation of the first seed culture solution

[0047] 1) Preparation of the first medium: Dilute 20g of glucose, 8g of yeast extract, 9g of peptone and 20g of agar to 1000mL with distilled water, keep the pH value natural, sterilize with 0.1MPa high-pressure steam for 20 minutes, and place the inclined plane to obtain the first a culture medium.

[0048] 2) Prepare the first seed: inoculate the Candida utilis (Candida utilis) strain purchased from the "China Industrial Microorganism Culture Collection Management Center" with the preservation number 1769 into the first medium in a sterile environment, at 35°C Cultivate statically for 48 hours under constant temperature conditions to obtain the first seed.

[0049] 3) Preparation of the first seed medium: 1000 mL of wor...

Embodiment 2

[0074] (1) Preparation of seed culture medium

[0075] A. Preparation of the first seed culture solution

[0076] 1) Prepare the first medium: 15g of glucose, 5g of yeast extract, 8g of peptone and 15g of agar were adjusted to 1000mL with distilled water, the pH value was natural, sterilized with 0.1MPa high-pressure steam for 20 minutes, and placed on an inclined plane.

[0077] 2) Preparation of the first seed: Inoculate the Candida tropicalis strain (Candida tropicalis) purchased from the "China Industrial Microbiological Strain Collection and Management Center" with the preservation number 1318 into the first medium in a sterile environment, and keep the temperature at 35°C Under the condition of static culture for 62h, the first seed was obtained.

[0078] 3) Preparation of the first seed medium: First seed medium: 1000 mL of 10°Brinx wort was placed in a Erlenmeyer flask with a natural pH value, and sterilized with 0.1MPa high-pressure steam for 20 minutes to obtain the...

Embodiment 3

[0102] (1) Preparation of seed culture medium

[0103] A. Preparation of the first seed culture solution

[0104] 1) Preparation of the first medium: 18g of glucose, 4g of yeast extract, 9g of peptone and 18g of agar were distilled to 1000mL with distilled water, the pH value was natural, sterilized with 0.1MPa high-pressure steam for 25 minutes, and then tilted to obtain the first medium .

[0105] 2) Preparation of the first seed: Inoculate the strain of Candida utilis (Candida utilis) into the first medium in a sterile environment, and culture it statically at a constant temperature of 36° C. for 72 hours to obtain the first seed.

[0106] 3) Preparation of the first seed medium: First seed medium: 1000 mL of 8°Brinx wort was placed in an Erlenmeyer flask with a natural pH value, and sterilized with 0.1MPa high-pressure steam for 20 minutes to obtain the first seed medium.

[0107] 4) Preparation of the first seed culture medium: Inoculate the first seed into the Erlenmey...

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PUM

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Abstract

The invention discloses a preparation method of an amino acid leaf fertilizer. The preparation method of the amino acid leaf fertilizer comprises the following steps of: A, uniformly mixing bean pulp with alcoholic fermentation wastewater, and sterilizing to obtain a fermentation medium; B, inoculating the first seed culture solution containing candida utilis or monilia tropicalis to the fermentation medium for fermentation; C, inoculating the second seed culture solution containing bacillus subtilis and the third seed culture solution containing aspergillus niger to the fermentation culture medium for fermentation; D, inoculating the fourth seed culture solution containing lactobacillus acidophilus to the fermentation medium for fermentation to obtain fermentation liquor; and E, filtering the fermentation liquor and separating solid out of the fermentation liquor, wherein the filter liquor is the amino acid leaf fertilizer. The amino acid content in the amino acid leaf fertilizer is greater than 8%, the content of the water insoluble is less than 5% and the pH value is between 4.0 and 7.5, which completely meet the requirements of National Standard GB/T17419-1998 amino acid containing leaf fertilizer.

Description

technical field [0001] The invention relates to the field of foliar fertilizer production, in particular to a preparation method of amino acid foliar fertilizer. Background technique [0002] Alcohol is an important industrial and food raw material, which is widely used in chemical industry, food and beverage, daily chemical and medical and health industries. It is also a widely used renewable energy source, so it has a very wide range of applications and development prospects. The alcohol fermentation industry is a relatively serious pollution industry. According to the conventional process, about 12-15 tons of wastewater is discharged for every ton of alcohol produced. The treatment of alcohol wastewater has become a bottleneck for the development of various alcohol enterprises. Alcohol wastewater contains a large amount of organic compounds and suspended solids, the main components are sugar, protein, vitamins, etc., and the COD is as high as 30000-60000mg / L, which is a t...

Claims

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Application Information

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IPC IPC(8): C05F5/00C05F11/08
CPCY02A40/20
Inventor 唐波李舒宇仝志勇陈玉和
Owner 江苏联海生物科技有限公司
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