Method for producing heptadecanedioic acid by fermenting and converting n-heptadecane

A technology for n-heptadecane and dibasic acid, which is applied in the field of high-yield heptadecane dibasic acid transformation from n-heptadecane fermentation, can solve the problem that industrial production is not realized, there is no economically feasible chemical synthesis method, and the technology is not mature enough. And other issues

Active Publication Date: 2011-07-06
青岛智库生物科技有限公司
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  • Summary
  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

Chen Yuantong, the successor of Fangyuan, has led the application and development of single long-chain dibasic acid series products since 1986. Over the past 20 years, he has obtained 7 patent authorization certificates, 4 of which have been industrialized. , the invention patent No. ZL89102548.0 discloses the fermentation production of DC 17 , the acid production level reaches 133g / l, which is the highest acid production level in similar research results at home and abroad, but from the perspective of industrial production, the technology is not mature enough to realize industrial production
while DC 17 It is an important raw material for the production of civet fragrance in the chemical industry. It does not exist in nature. So far, there is no economically feasible chemical synthesis method at home and abroad.

Method used

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] (1) An inoculation loop of Candida tropicalis ly-7 cells was taken, spread on the wort solid slope of a 15×180 large test tube, and cultivated at 28° C. for 2 days.

[0029] (2), get one of the above strains, insert it into a 250ml Erlenmeyer flask with 30ml liquid seed culture medium and cultivate it for 40 hours at 28-30°C on a rotary shaker at 220 rpm. Liquid seed medium contains KH 2 PO 4 8g / l, yeast extract 5g / l, corn steep liquor 3g / l, sucrose 30g / l, urea 3g / l, tap water, pH 5.0.

[0030] (3) In a 500ml Erlenmeyer flask containing 15ml of fermentation medium, insert 3.5ml of the above-mentioned cultivated seed solution, ferment for 4 days at 28-30°C, 220 rpm on a rotary shaker, and use 6N every 24 hours Adjust the pH to 7.5-8.0 once with NaOH. The fermentation medium mixture contains KH 2 PO 4 10g / l, sucrose 20 / l, yeast extract 3g / l, corn steep liquor 3.5g / l, urea 1.5g / l, and nC 17 200ml / l, tap water configuration, PH7.2. Sterilize at 110°C for 30 minutes. ...

Embodiment 2

[0032] According to the method of embodiment 1, just fermentation substrate n-alkane uses decane (nC 10 ), the result DC 10 The yield was 35 g / l with a purity of 96.2%.

Embodiment 3

[0034] According to the method of embodiment 1, just fermentation substrate n-alkane uses n-tridecane (nC 13 ), the result DC 13 The yield is 70g / l with a purity of 97.8%

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Abstract

The invention discloses a method for producing long-chain alpha,omega-dicarboxylic acid by fermenting and converting an n-alkane with microbes, particularly a method for producing high-yield heptadecanedioic acid, which comprises the following steps: inoculating seed liquor, which is cultured from a Candida tropicalis mutant strain ly-7 which is obtained by mutagenesis by an N<+> injection technique and subjected to oxidase activity screening and culture, into mixed liquor of an n-alkane and a fermentation medium; fermenting and converting at 25-32 DEG C for 48-168 hours; and separating and purifying the generated long-chain alpha,omega-dicarboxylic acid. When n-heptadecane is fermented and converted to produce the heptadecanedioic acid in a 5-ton fermentation tank for 144 hours, the content of the heptadecanedioic acid can reach 163 g / l.

Description

Technical field: [0001] The present invention relates to the method for producing long-chain dicarboxylic acid with biosynthesis technology, specifically the method for producing long-chain α, ω-dicarboxylic acid from normal heptadecane (nC 17 ) fermentative conversion of high-yield seventeen carbon dibasic acid (DC 17 )Methods. technical background: [0002] Long-chain dibasic acids refer to aliphatic dicarboxylic acids containing more than 10 carbon atoms in the carbon chain, including C 11 -C 18 A series of dibasic acid products, which have extremely extensive and important uses, are used in the chemical industry to manufacture high-performance nylon engineering plastics, advanced coatings, emerging medicines, high-grade nylon hot-melt adhesive high-temperature media, biofilms and pesticides, etc. industry, an important raw material for dozens of high-end products. Long-chain dibasic acids do not exist in nature, and chemical methods can only synthesize dodecane dibas...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/44C12R1/74
Inventor 曹务波陈远童曹荀梅子
Owner 青岛智库生物科技有限公司
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