Method for synchronously fermenting n-undecane (nC11) to highly yield eleven-carbon dicarboxylic acid (DC11) by utilizing microorganism
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A technology of simultaneous fermentation and carbodicarboxylic acid, which is applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., and can solve problems such as no chemical synthesis method.
Active Publication Date: 2011-07-06
青岛智库生物科技有限公司
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So far, there is no economically feasible chemical synthesis method at home and abroad
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Embodiment 1
[0037] (1). Take an inoculation loop of Candida tropicalis ly-1 cells, spread it on the wort solid slope of a 15×180 large test tube, and cultivate it at 30° C. for two days.
[0038] (2). Take one strain cultured in (1), insert it into a 250mL Erlenmeyer flask containing 25mL mixed liquid seed medium, and cultivate it on a rotary shaker at 30°C and 220rpm for 48 hours. Mixed Liquid Seed Medium, KH 2 PO 4 8g / L, yeast extract 5g / L, corn steep liquor 3g / L, sucrose 25g / L, urea 3g / L, prepared with tap water, pH 5.0.
[0039] (3) In a 500mL Erlenmeyer flask containing 15mL of fermentation medium, insert 3.5mL of the seed solution cultivated in (2), ferment on a rotary shaker at 220rpm at 30°C for 4 days, and adjust with NaOH every 24 hours pH to 7.8. Fermentation medium with KH 2 PO 4 8g / L, NaCL 1g / L, yeast extract 3g / L, corn steep liquor 3.5g / L, urea 1.5g / L, KNO 3 7g / L, foam enemy 400ppm, and n-undecane (nC 11 ) 200mL / L, prepared with tap water, pH 7.1, sterilized at 110...
Embodiment 2
[0041] According to the method of embodiment 1, just wherein fermentation substrate nC 11 use nC 13 Instead, the resulting DC 13 The yield is 53.6g / L, and the purity is 96.8%.
Embodiment 3
[0043] According to the method of embodiment 1, just wherein fermentation substrate nC 11 use nC 15 Instead, the resulting DC 15 The yield is 58.5g / L, and the purity is 97.2%.
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Abstract
The invention discloses a method for synchronously fermenting n-undecane (nC11) to highly yield eleven-carbon dicarboxylic acid (DC11) by utilizing a microorganism. The microorganism is Candida tropicalis ly-1, of which the preservation number is CCTCC (China Center for Type Culture Collection) NO. M 209223. The method is characterized in that within 28 hours after strains of the microorganism are connected to a culture medium using normal alkane as the substrate, the pH (hydrogen ion concentration) is controlled below 6.8, cell growth is taken as the principal task, and a certain amount of dicarboxylic acid is also produced; within 28 hours to 60 hours, the pH is controlled below 7.3, acid production is taken as the principal task, and a certain number of cells are also increased; and after 60 hours, the pH is controlled below 8.0, and various dicarboxylic acids are produced quickly. When the method is used for fermenting nC11 to produce DC11, the stains of the microorganism and nC11are fermented for 150 hours in a fermentation tank with a volume of 210m3; and the output of DC11 can reach up to 156g/L, the conversion rate can reach 86.7 percent, and the purity of DC11 can reach 98.2 percent.
Description
Technical field: [0001] The present invention relates to the method for the production of long-chain α, ω-dibasic acid of microorganism synchronous fermentation n-alkane, especially fermentation n-undecane (hereinafter referred to as nC 11 ) high-yield α, ω-undecanedicarboxylic acid (hereinafter referred to as DC 11 )Methods. Background technique: [0002] C 10 The above long-chain dibasic acids are important raw materials for the synthesis of high-grade spices, high-grade nylon engineering plastics, nylon hot-melt adhesives for high-grade clothing, high-temperature electrolytes, high-grade coatings, lubricating oils and cold-resistant plasticizers in the chemical industry, especially thirteen carbon dibasic acid (DC 13 ) and pentadecanedioic acid (DC 15 ), which are important raw materials for synthesizing daily fragrance musk-T and luxury fragrance muscone respectively. [0003] C 10 The above long-chain dibasic acids do not exist alone in nature, only a few kinds of...
Claims
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